Navigation Links
Iowa State, Ames Lab researchers study the structure of drug resistance in tuberculosis
Date:8/22/2012

AMES, Iowa Edward Yu took note of the facts nearly 2 million deaths each year, 9 million infected each year, developments of multidrug-resistant, extensively drug-resistant and now totally drug-resistant strains and decided to shift his research focus to tuberculosis.

Yu, an Iowa State University and Ames Laboratory researcher, has described in the journal Nature the three-part structure that allows E. coli bacteria to pump out toxins and resist antibiotics.

And now, in a paper published online by the journal Nucleic Acids Research, a research team led by Yu describes the structure of a regulator that controls the expression of the multidrug efflux pump in Mycobacterium tuberculosis.

Yu a professor of physics and astronomy, of chemistry, of biochemistry, biophysics and molecular biology in Iowa State's College of Liberal Arts and Sciences and an associate of the U.S. Department of Energy's Ames Laboratory said the latest study is a starting point for a better understanding of how the tuberculosis bacterium is able to resist drugs.

The development of strains totally resistant to drugs "inspired us to move in this direction and try to understand the mechanism in developing drug resistance," Yu said.

"It is obvious that the emergence of these drug-resistant TB strains has evolved into a major threat and challenges our global prospects for TB control," Yu's research team wrote in its latest paper. "Thus, knowledge of the molecular mechanisms underlying drug resistance in M. tuberculosis is essential for the development of new strategies to combat this disease."

Yu's research is currently supported by the National Institutes of Health. The researchers' use of the Advanced Photon Source at Argonne National Laboratory in Argonne, Ill., was supported by the U.S. Department of Energy's Office of Basic Energy Sciences.

In addition to Yu, the research team includes Qijing Zhang, Iowa State's Frank Ramsey Endowed Professor of Veterinary Microbiology and Preventive Medicine and the College of Veterinary Medicine's officer of graduate education; Kanagalaghatta Rajashankar, a senior research associate in chemistry and chemical biology at Cornell University in Ithaca, N.Y., and associate director of the Northeastern Collaborative Access Team facility at the Advanced Photon Source; Iowa State post-doctoral research associates and Ames Lab associates Feng Long and Chih-Chia Su; Iowa State post-doctoral research associate Lei Dai; Iowa State graduate students and Ames Lab student associates Jani Reddy Bolla, Sylvia Do and Hsiang-Ting Lei; recent Iowa State graduate Xiao Chen; and Ames Lab undergraduate summer interns Jillian Gerkey and Daniel Murphy.

Prior to Yu's study, not much was known about the structure and function of the tuberculosis efflux pump regulator known as Rv3066.

That, in part, is because researchers have attributed drug resistance in tuberculosis to the bacterium's very thick cell wall. That wall makes it very difficult to get drugs into the bacterium.

The researchers used X-ray crystallography (including X-ray beams produced by the Advanced Photon Source) to study the Rv3066 structure. They collected data showing the regulator when the toxic compound ethidium bromide was present and when it was not.

The data revealed an asymmetric, two-part molecule with a spiral structure. The structure is flexible, allowing the regulator to recognize and respond to multiple drugs. In the presence of ethidium, Yu's group says the regulator responds with a rotational motion, inducing expression of the efflux pump that rids the bacterium of antimicrobial drugs.

Studying that structure and mechanism could make a difference in the fight against drug-resistant tuberculosis: "Elucidating the regulatory systems of multidrug efflux pumps in M. tuberculosis," Yu and the researchers wrote in their paper, "should allow us to understand how this bacterium contributes to multidrug resistance and how it adapts to environmental changes."


'/>"/>
Contact: Edward Yu
ewyu@iastate.edu
515-294-4955
Iowa State University
Source:Eurekalert  

Related biology news :

1. Iowa State, Ames Lab researchers invent new tool to study single biological molecules
2. ORNL researchers improve soil carbon cycling models
3. Researchers identify key culprit causing muscle atrophy
4. Researchers demonstrate control of devastating cassava virus in Africa
5. Researchers pursue red flag for schizophrenia relapse
6. A new line of defense: Researchers find cattle vaccine works to reduce E. coli O157:H7
7. Wayne State researchers working to improve genetic analysis, disorder detection
8. Superbird stuns researchers
9. Massachusetts Eye and Ear researchers discover elusive gene that causes a form of blindness from birth
10. Researchers monitor red tides in Chesapeake Bay
11. BUSM researchers find link between childhood abuse and age at menarche
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Iowa State, Ames Lab researchers study the structure of drug resistance in tuberculosis
(Date:3/22/2016)... , PROVO and ... Newborn Screening Ontario (NSO), which operates the highest sample ... molecular testing, and Tute Genomics and UNIConnect, leaders in ... respectively, today announced the launch of a project to ... (NGS) testing panel. NSO has been ...
(Date:3/17/2016)... , March 17, 2016 ABI ... intelligence, forecasts the global biometrics market will reach ... impressive 118% increase from 2015. Consumer electronics, particularly ... embedded fingerprint sensors anticipated to reach two billion ... Dimitrios Pavlakis , Research Analyst at ...
(Date:3/14/2016)... March 14, 2016 NXTD ) ("NXT-ID" ... commerce market, announces the airing of a new series of ... week of March 21 st .  The commercials will air ... popular Squawk on the Street show. --> NXTD ... growing mobile commerce market, announces the airing of a new ...
Breaking Biology News(10 mins):
(Date:5/25/2016)... (PRWEB) , ... May 25, 2016 , ... ... Michael Fitzmaurice recently became double board-certified in surgery and surgery of the hand ... Dr. Fitzmaurice is no stranger to going above and beyond in his pursuit ...
(Date:5/25/2016)... ... 2016 , ... Lady had been battling arthritis since the age of two ... knee. Lady’s owner Hannah sought the help of Dr Jeff Christiansen of Superior ... cruciate ligament and help with the pain of Lady’s arthritis. Dr Christiansen suggested that ...
(Date:5/24/2016)... La Jolla, CA (PRWEB) , ... May 24, 2016 , ... ... and financial planning for corporate executives and entrepreneurs, held The Future of San Diego ... leaders in the San Diego life science community attended the event with speakers Dr. ...
(Date:5/23/2016)... WARSAW, Ind. , May 23, 2016 Zimmer ... in musculoskeletal healthcare, today announced that its Board of Directors ... stockholders for the second quarter of 2016. ... on or about July 29, 2016 to stockholders of record ... Future declarations of dividends are subject to approval of the ...
Breaking Biology Technology: