Navigation Links
Iowa State, Ames Lab researchers find the mechanism that forms cell-to-cell catch bonds
Date:6/5/2014

AMES, Iowa Certain bonds connecting biological cells get stronger when they're tugged. Those bonds could help keep hearts together and pumping; breakdowns of those bonds could help cancer cells break away and spread.

Those bonds are known as catch bonds and they're formed by common adhesion proteins called cadherins. Sanjeevi Sivasankar, an Iowa State University assistant professor of physics and astronomy and an associate of the U.S. Department of Energy's Ames Laboratory, has described catch bonds as "nanoscale seatbelts. They become stronger when pulled."

But how does that happen? How can bonds get stronger under force?

Sivasankar and his research team have found long-lived, force-induced hydrogen bonds are the answer. A paper describing their findings, "Resolving the molecular mechanism of cadherin catch bond formation," has just been published online by Nature Communications.

Sivasankar is the corresponding author. Co-authors are Kristine Manibog, an Iowa State graduate student in physics and astronomy and a student associate of the Ames Laboratory; Hui Li, of the Suzhou Institute of Biomedical Engineering and Technology of the Chinese Academy of Sciences in Suzhou New District, China; and Sabyasachi Rakshit, of the Indian Institute of Science Education and Research in Mohali, India. Li and Rakshit are former postdoctoral researchers in Sivasankar's laboratory.

The team's research was supported by grants from the American Cancer Society and the American Heart Association.

Sivasankar said strong cell-to-cell bonds are important to heart health and fighting cancer. He said the bonds connecting heart cells have to withstand constant mechanical forces. And, in some cancers, he said bonds no longer resist forces, allowing cancer cells to detach and spread.

To find the mechanism behind the strong ties created by catch bonds, Sivasankar's research team began with molecular dynamics and steered molecular dynamics computer simulations based on data from previous experiments. They found that two rod-shaped cadherins bound together in an X-shape (called an X-dimer) form catch bonds when pulled and in the presence of calcium ions.

The calcium ions keep the cadherins rigid and ordered while the pulling brings parts of the proteins closer together. All of that allows a series of hydrogen bonds to form. These long-lived, force-induced hydrogen bonds lock the X-dimers into tighter contact.

Sivasankar said the researchers followed up the simulations with single-molecule experiments using atomic force microscopy. The experiments confirmed that cadherin X-dimers, when pulled and exposed to high calcium ion concentrations, formed catch bonds. Take away the force or the calcium ions, and catch bond formation was eliminated.

All of this, Sivasankar said, helps explain the biophysics of cell-to-cell adhesion. And that's important to all of us.

"Robust cadherin adhesion," the researchers wrote in their paper, "is essential for maintaining the integrity of tissue such as the skin, blood vessels, cartilage and muscle that are exposed to continuous mechanical assault."


'/>"/>

Contact: Sanjeevi Sivasankar
sivasank@iastate.edu
515-294-1220
Iowa State University
Source:Eurekalert  

Related biology news :

1. Iowa State, Ames Lab researchers invent new tool to study single biological molecules
2. Iowa State, Ames Lab researchers study the structure of drug resistance in tuberculosis
3. Iowa State, Ames Lab researchers find 3 unique cell-to-cell bonds
4. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
5. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
6. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
7. UNH researchers find African farmers need better climate change data to improve farming practices
8. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
9. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
10. Researchers print live cells with a standard inkjet printer
11. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Iowa State, Ames Lab researchers find the mechanism that forms cell-to-cell catch bonds
(Date:5/9/2016)... May 9, 2016 Elevay is ... to expanding freedom for high net worth professionals seeking ... today,s globally connected world, there is still no substitute ... ever duplicate sealing your deal with a firm handshake. ... by taking advantage of citizenship via investment programs like ...
(Date:4/28/2016)... BANGALORE, India , April 28, 2016 ... subsidiary of Infosys (NYSE: INFY ), and Samsung ... global partnership that will provide end customers with a ... and payment services.      (Logo: http://photos.prnewswire.com/prnh/20130122/589162 ... for financial services, but it also plays a fundamental part ...
(Date:4/26/2016)... India and LONDON ... Infosys Finacle, part of EdgeVerve Systems, a product ... and Onegini today announced a partnership to integrate ... solutions.      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ... to provide their customers enhanced security to access ...
Breaking Biology News(10 mins):
(Date:5/23/2016)... and LONDON , May 23, 2016 ... Could See Frontage Boost Efficiency by 40% - Frontage ... - Frontage Enforce Quality, Compliance and Traceability Within the Bioanalytical ... with labs in the United States and ... to be deployed across its laboratory facilities. In addition to ...
(Date:5/23/2016)... ... 23, 2016 , ... Foresight Institute , a leading ... for the 2015 Foresight Institute Feynman Prizes. , These prestigious prizes, named ... for experiment and the other for theory in nanotechnology. Prof. Markus J. Buehler, ...
(Date:5/20/2016)... CA (PRWEB) , ... May 20, 2016 , ... The ... 10 of its most experienced veterinary clients have treated over 100 of their own ... edge technology to provide the highest level of care for their patients. , ...
(Date:5/20/2016)... NC (PRWEB) , ... May 20, 2016 , ... Korean ... suggesting that it may offer a new way to treat the disease. Surviving Mesothelioma ... it now. , Scientists from several Korean institutions based their mesothelioma study on ...
Breaking Biology Technology: