Navigation Links
In subglacial lake, surprising life goes on
Date:7/5/2013

ng culturing colonies of bacteria and fungi, but the process was slow, especially for graduate students who needed results for their theses.

"We started thinking of doing it a different way," Rogers said.

Instead of culturing living organisms, they concentrated on sequencing DNA and RNA in the ice. These methods, called metagenomics and metatranscriptomics, produced thousands of sequences at a time that were then analyzed using computers procedures referred to collectively as "Big Data" methods. In contrast, it usually took years to generate enough cultured organisms for a few dozen sequences.

The problem changed from having too few sequences to having too many sequences to analyze, Rogers said. After two years of computer analysis, the final results showed that Lake Vostok contains a diverse set of microbes, as well as some multicellular organisms.

Long before he began using metagenomics and metatranscriptomics to study the ice, Rogers and his team had developed a method to ensure purity. Sections of core ice were immersed in a sodium hypochlorite (bleach) solution, then rinsed three times with sterile water, removing an outer layer. Under strict sterile conditions, the remaining core ice was then melted, filtered and refrozen.

"Using this method, we can assure its reliability almost to 100 percent," Rogers said.

Eventually, the process rendered pellets of nucleic acids containing both DNA and RNA, able to be sequenced.

Rogers said the team erred strongly on the conservative side in reporting its results, including only those sequences of which it could be absolutely certain were from the accretion ice, but there are a multitude of others he feels are probably from the lake, opening the door to additional investigation.

The DNA sequences they produced have been deposited in the National Center for Biotechnology GenBank database, and will be available to other researchers for further study
'/>"/>

Contact: Jen Sobolewski
jsobole@bgsu.edu
Bowling Green State University
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. Genetic survey of endangered Antarctic blue whales shows surprising diversity
2. Surprising pine beetle breeding habits help explain increasing tree damage, says CU study
3. A surprising new kind of proton transfer
4. First-of-its-kind study reveals surprising ecological effects of earthquake and tsunami
5. Seabirds study shows plastic pollution reaching surprising levels off coast of Pacific Northwest
6. Nuclear weapons surprising contribution to climate science
7. Surprising demographic shifts in endangered monkey population challenge conservation expectations
8. Despite their thick skins, alligators and crocodiles are surprisingly touchy
9. Surprising teaching tool in K-12 science education -- Zebrafish research
10. Climate changes effects on temperate rain forests surprisingly complex
11. New look at cell membrane reveals surprising organization
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/11/2014)... international team of researchers, including scientists from the Max ... a reported a major step in understanding photosynthesis, the ... maintains the oxygen in its atmosphere and which is ... earth. , The researchers report the first direct ... namely the step in which a specific protein complex, ...
(Date:7/11/2014)... 11, 2014 The increased risk of kidney injury ... resuscitation fluids reflects the mass of HES molecules, according ... official journal of the International Anesthesia Research Society (IARS). ... harmful effect of HES on cultured human renal proximal ... Christian Wunder and colleagues of University Hospital Wrzburg, Austria. ...
(Date:7/11/2014)... under the normal circumstances, astrocytes participate in ... environment, and exhibit therapeutic and repairing effects ... studies have found that nerve cells differentiated ... have reduced viability, which produces influences on ... Kailuan General Hospital, Hebei United University, China ...
Breaking Biology News(10 mins):A first direct glimpse of photosynthesis in action 2In lab studies, hydroxyethyl starch has direct harmful effects on kidney cells 2
... Soil is the most species-rich microbial ecosystem ... certain species, give them access to the root and so ... then benefit in a variety of ways. To achieve this, ... of these bacteria are friends and which foes. Researchers from ...
... reported details of how certain drugs can precisely target ... replication. Since all strains of the virus require the ... drugs that can effectively treat new strains of the ... When new strains of influenza emerge, it can ...
... Results from a study conducted at Georgia State University ... intestines and the immune system, kicked off by another type ... disease. The study suggests that the "fight" continues after ... to Andrew Gewirtz, professor of biology at the GSU Center ...
Cached Biology News:Bacterial community inside the plant root 2Bacterial community inside the plant root 3Target for potent first-strike influenza drugs identified 2Target for potent first-strike influenza drugs identified 3Target for potent first-strike influenza drugs identified 4
(Date:7/11/2014)... July 11, 2014 Research and Markets ... Technology Market - Global Industry Analysis, Size, Share, Growth, ... offering. http://photos.prnewswire.com/prnh/20130307/600769 ... acids (either DNA or RNA) into cells. This technology ... cancer cells and protein metabolism by affecting the nuclear ...
(Date:7/11/2014)... , July 11, 2014  Researchers have ... three-dimensional images. The new technology, called Virtual Finger, ... small structures like neurons and synapses using the ... unique technology makes 3D imaging studies orders of ... at an unprecedented level across many areas of ...
(Date:7/10/2014)... Deep Knowledge Ventures last week ... ‘Commercialising Longevity Research’ and welcomed a host of ... Bioscience Innovation Center for the event, organised by ... Ltd. The meeting highlighted the need for both ... age-related disease, as well as the crucial role ...
(Date:7/10/2014)... Robert Harman, DVM, Founder and CEO of Vet-Stem, Inc., ... the relaunch of his highly informative blog, now named Stem ... What are Stem Cells ?” Dr. Harman’s purpose for ... the basics of stem cell therapy so that pet owners ... treatment when considering regenerative medicine. , A veterinarian by trade, ...
Breaking Biology Technology:International Transfection Technology Market - Industry Analysis, Size, Share, Growth, Trends and Forecast to 2019 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 5DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 6Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3
... CLEVELAND, Jan. 28, 2011 Arteriocyte®, a leading ... Ohio and Hopkinton, Massachusetts that develops proprietary stem ... approval from the Food and Drug Administration (FDA) ... its Magellan MAR01™ technology in the treatment of ...
... Reportlinker.com announces that a new ... its catalogue: ... Probes and Biochips -- New Product ... for Instrument and Reagent Suppliers ...
... Calif. and HOPKINTON, Mass., Jan. 27, 2011 ... (Nasdaq: CALP ) today announced a ... for next generation sequencing sample preparation on Caliper,s ... researchers to leverage NuGEN,s Ovation® and Encore™ reagent ...
Cached Biology Technology:Arteriocyte Receives FDA Approval to Move Forward with Critical Limb Ischemia Trial 2Arteriocyte Receives FDA Approval to Move Forward with Critical Limb Ischemia Trial 3Reportlinker Adds The 2011 Molecular Diagnostics Market: DNA Probes and Biochips -- New Product Development Opportunities and Business Expansion Strategies for Instrument and Reagent Suppliers 2NuGEN and Caliper Partner to Eliminate Next-Gen Sequencing Sample Preparation Bottlenecks 2NuGEN and Caliper Partner to Eliminate Next-Gen Sequencing Sample Preparation Bottlenecks 3
PCR System for increased fidelity, yield and length of PCR products. Amplifies targets up to 10 kb from human genomic DNA....
Immunogen: Heat inactivated bacterial cells of Salmonella enteriditis,paratyphi and typhimurium Storage: 4 C...
... Immunogen: Recombinant protein ... region of human ubiquilin. ... the human ubiquilin protein. ... control: 293 cells). ...
...
Biology Products: