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Improving survival of pig sperm
Date:4/24/2013

URBANA Although U.S. cattle genetics are exported all over the world in the form of frozen semen, the same is not true for pigs because boar semen does not freeze well. In an attempt to improve semen storage and pig reproduction, animal scientists at the University of Illinois are looking at how sperm survives in the sow oviduct.

"Many mammals and birds will store sperm for some period of time," said associate professor of animal sciences David Miller. "Pigs will store sperm for 24 to 48 hours." Sperm must be stored in species in which mating and ovulation are poorly synchronized.

Miller and his team wanted to understand the adhesion system that retains the sperm. Previous research indicated that sugars in the oviduct were an important part of the process, but it was not clear which ones.

They screened hundreds of sugars, using an array on a microscope slide to which they added fluorescently labeled boar semen. When the semen bound to a sugar, fluorescence was visible on the slide. They identified two sugars: sialylated lactosamine and Lewis X.

The next step is to identify the receptor on the sperm.

"We'd like to know exactly which molecules the sugars bind," Miller said. "Then maybe we could develop a laboratory test for those molecules. If sperm have a lot of them, then maybe it would be possible to inseminate only once rather than two or three times, as is typical when inseminating sows."

He also noted that a better understanding of the storage process might also help them to determine what goes wrong when sperm are less fertile. If the problem is linked to the storage process, it might be possible to develop therapies to correct it.

Another question to explore is how the sugars lengthen sperm life span. If this could be determined, it might be possible to extend the time that semen can be stored, making it easier to ship it to other places. "If we can improve storage and transportation, we c
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Contact: Susan Jongeneel
sjongene@illinois.edu
217-333-3291
University of Illinois College of Agricultural, Consumer and Environmental Sciences
Source:Eurekalert

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