Navigation Links
Improved method for capturing proteins holds promise for biomedical research
Date:8/16/2011

Antibodies are the backbone of the immune systemcapable of targeting proteins associated with infection and disease. They are also vital tools for biomedical research, the development of diagnostic tests and for new therapeutic remedies.

Producing antibodies suitable for research however, has often been a difficult, costly and laborious undertaking.

Now, John Chaput and his colleagues at the Biodesign Institute at Arizona State University have developed a new way of producing antibody-like binding agents and rapidly optimizing their affinity for their target proteins. Such capture reagents are vital for revealing the subtleties of protein function, and may pave the way for improved methods of detecting and treating a broad range of diseases.

The team's results appear in today's issue of the journal ChemBioChem.

Antibodies are Y-shaped structures, capable of binding in two or more places with specific target proteins. Synthetic antibodies are much simpler forms that attempt to mimic this behavior. As Chaput explains, creating affinity reagents with strong binding properties can be accomplished by combining two weak affinity segments on a synthetic scaffold. The resulting affinity reagent, if properly constructed, can amplify the binding properties of the individual segments by two or three orders of magnitude.

"This dramatic change in affinity has the ability to transform ordinary molecules into a high affinity synthetic antibody," Chaput says. "Unfortunately, the chemistry used to make these reagents can be quite challenging and often requires a lot of trial-and-error. With NIH funding, my group has reduced the complexity of this problem to simple chemistry that is user friendly and easily amenable to high throughput automation. Such technology is absolutely necessary if we want to compete with traditional monoclonal antibody technology. "

Traditionally, antibodies for research have been extr
'/>"/>

Contact: Joseph Caspermeyer
joseph.caspermeyer@asu.edu
Arizona State University
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. U of M researchers use improved imaging technique; discover a better approach to diagnosing epilepsy
2. Trastuzumab and chemotherapy improved survival in HER2-postive breast and brain cancer patients
3. New genetic map of potato may lead to improved crops
4. Nanowire-based sensors offer improved detection of volatile organic compounds
5. China needs improved administrative system for nuclear power safety
6. IOF calls for improved strategies to close the treatment gap and reduce future burden of fractures
7. NPL helps Senceive to offer improved monitoring of structural assets across the UK
8. NIST seeks improved recovery of samples from biohazard events
9. Recycling perlite: New, improved method saves resources
10. Detecting pathogens in waterways: An improved approach
11. Pertuzumab and trastuzumab combination improved efficacy for women with HER2-positive breast cancer
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Improved method for capturing proteins holds promise for biomedical research
(Date:3/24/2015)... 24, 2015 Research and Markets ( ... "Global Iris Recognition Market 2015-2019" report to their ... Recognition market to grow at a CAGR of 23.5 ... covers the present scenario and the growth prospects of ... To calculate the market size, the report considers the ...
(Date:3/24/2015)... , March 24, 2015   Crossmatch ™, ... today announced that its U.are.U ® 4500 ... Goorin Bros . to achieve PCI compliance ... secures access to the POS terminal, protecting the ... of data breaches. With one-third ...
(Date:3/23/2015)... 2015  In the 2014 fiscal year, irs.gov recorded ... This figure accounts for a fraction of the estimated ... Report, exceeds $11 billion globally. The polygraph has traditionally ... has occurred. Investor Mark Saint Juste along ... Paolella partnered to administer the test in a ...
Breaking Biology News(10 mins):Global Iris Recognition Market 2015-2019 with 3M Cogent, Bioenable Technologies, Cross Match Technology, Iris ID Systems & Safran Dominating 2Crossmatch Fingerprint Readers Help Retailer Prevent Data Breaches at Point-of-Sale 2Polygraph Test Now Used to Protect Investors From Fraud 2Polygraph Test Now Used to Protect Investors From Fraud 3
... Dr Veerle Huvenne of the United Kingdom,s National Oceanography Centre ... million Euros over five years to map complex habitats in ... The European Research Council (ERC) ,Starting Grant, scheme aims to ... own research groups. The grants are highly competitive, and this ...
... October 27, 2010 TEL AVIV, Israel Assembled ... Israel under the EUREKA Chairmanship year, EUREKA,s High Level ... of promising cooperative R&D projects in a variety of ... accordance with EUREKA,s strategic targets defined for the 2010-2011 ...
... Human Reproduction and Embryology (ESHRE) has published the first textbook ... English language. A Dutch textbook, in layout and ... higher professional education (Master,s level), was the basis for this ... nurses working in reproductive health. For a net ...
Cached Biology News:European R&D leaders assembled in Tel Aviv will invest $70 million in joint R&D initiatives 2
(Date:4/17/2015)... - SQI Diagnostics Inc. (TSX-V: SQD; OTCQX: SQIDF), a ... commercializes proprietary technologies and products for advanced microarray ... approval from the TSX Venture Exchange to extend ... warrants ("the Warrants") of the Company, which were ... private placement financing. Each Warrant entitles ...
(Date:4/17/2015)... , April 17, 2015  US-Australian drug discovery company, ... Memorandum of Understanding with the Feinstein Institute for ... York to collaborate with the objective ... The collaboration brings together the drug discovery ... expertise of the Feinstein Institute in neurosciences and ...
(Date:4/17/2015)... (PRWEB) April 17, 2015 LC ... proteomics technologies, announced today that it is seeking ... an innovative multiplex PCR based targeted sequencing technology. ... sequence analysis, that enables accurate detection of rare ... and Omega-Primerâ„¢ technologies. The company will collaborate with ...
(Date:4/16/2015)... Carolina (PRWEB) April 17, 2015 ... steam system services, released their newly designed, state-of-the-art ... The customer facing website effectively promotes the Spirax ... , All the information that a site visitor ... system services, company capabilities, industries serviced, the latest ...
Breaking Biology Technology:SQI Diagnostics to Extend Warrant Expiry 2SQI Diagnostics to Extend Warrant Expiry 3Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 2Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 3Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 4Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 5Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 6LC Sciences Seeks Beta Testers of Targeted Sequencing Method Ahead of Q4 Launch 2LC Sciences Seeks Beta Testers of Targeted Sequencing Method Ahead of Q4 Launch 3Spirax Sarco website has a new look 2
... Entrepreneurial Excellence in Emerging Category, LOS ANGELES, ... announced that Dr. Donald Rice, Founder, President, & ... Year(R) 2008 Award in the,Emerging category in the ... LLP, the award recognizes outstanding entrepreneurs who are ...
... Pathway Would Also Lower Total,Expenditures for Biologics by $25 ... The Congressional Budget,Office (CBO) today released a cost analysis ... biologics -- or biogenerics -- would save,the federal government ... biologics by $25 billion over the same time period., ...
... advanced formula,scar gel is a unique patented silicone ... standard for scar treatment and abnormal scar,prevention. The ... is ONLY available from Advanced Bio-Technologies, Inc.(ABT)., ... label. Doctors,and customers should not be misled by ...
Cached Biology Technology:Agensys Founder, President, & CEO Dr. Donald Rice Named Ernst & Young Entrepreneur Of The Year(R) 2008 Award Winner for the Greater Los Angeles Area 2Agensys Founder, President, & CEO Dr. Donald Rice Named Ernst & Young Entrepreneur Of The Year(R) 2008 Award Winner for the Greater Los Angeles Area 3CBO: Biogenerics Would Save Federal Government $6.6 Billion Over Ten Years 2
ROCK-1 (cleaved) Monoclonal Ab 100 ug purified mouse monoclonal antibody. Clone# 12M03. Reacts with human. Tested in Western blot analysis....
Mouse monoclonal [LPL4A.1] to Plastin L ( Abpromise for all tested applications). SwissProtID: P13796...
Mouse monoclonal [CAT-1] to Chloramphenicol Acetyltransferase ( Abpromise for all tested applications)....
... Cells are designed for use with the ... strain MaV203 contains deletions in the endogenous ... GAL4-based two-hybrid systems. The strain also has ... ProQuest bait and prey vectors. MaV203 contains ...
Biology Products: