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Improved characterization of nanoparticle clusters for EHS and biosensors research
Date:10/26/2011

fuge causes the nanoparticle clusters to separate by size, the smaller, lighter clusters moving more slowly than the larger ones. While this is happening, the sample containers are repeatedly scanned with light and the amount of light passing through the sample for each color or frequency is recorded. The larger the cluster, the more light is absorbed by lower frequencies. Measuring the absorption by frequency across the sample containers allows the researchers both to watch the gradual separation of cluster sizes and to correlate absorbed frequencies with specific cluster sizes.

Most previous measurements of absorption spectra for solutions of nanoparticles were able only to measure the bulk spectrathe absorption of all the different cluster sizes mixed together. AUC makes it possible to measure the quantity and distribution of each nanoparticle cluster without being confounded by other components in complex biological mixtures, such as proteins. The technique previously had been used only to make these measurements for single nanoparticles in solution. The NIST researchers are the first to show that the procedure also works for nanoparticle clusters.


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Contact: Michael E. Newman
michael.newman@nist.gov
301-975-3025
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

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