Navigation Links
Impact of stem cell transplantation location in brain a crucial factor for cell survival
Date:2/13/2013

Putnam Valley, NY. (Feb. 13, 2013) Researchers at the Johns Hopkins University in Baltimore, Md., and the Mossakowski Medical Research Centre in Warsaw, Poland, have found that nonself-donated cells (allografts) better survive implantation into the brains of immunocompetent research mice when the grafts are injected into the striatum (STR) of the brain rather than injected into the forceps minor (FM) region. In their study, all FM grafts were rejected while STR grafts accumulated and survived along the border between the striatum and the corpus callosum.

"To the best of our knowledge, this is the first demonstration that allograft survival within the central nervous system of an immunocompetent, non-immune suppressed host may be highly dependent on implantation site," said study co-author Dr. Pior Walczak of the Johns Hopkins University School of Medicine.

The study appears as an early e-publication for the journal Cell Transplantation, and is now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/pre-prints/ct0888janowski.

The researchers, who assessed the grafted cells using bioluminescence for 16 days, reported that the distribution of the FM grafts was cylindrical, parallel to the needle track, while cells transplanted to the STR accumulated along the border between the striatum and the corpus callosum in a wedge-shaped, semi lunar "pocket." They suggested that the shape of the cell deposit in the FM was likely due to damage caused by the injection procedure.

"This is an indicator that surgical injury may be the leading factor initiating the rejection process," the researchers said. "Thus, minimally traumatic allograft transplantation, preventing activation of the microglia and limiting foreign antigen presentation, may contribute to long-term allograft survival."

They commented that the STR grafts were transplanted closer to the ventricles than were the FM grafts, suggesting that the lateral ventricles "appear to present a favorable microenvironment." Additionally, they hypothesized that variability in cell survival may be related to the differences in immune responses that may exist between white matter and grey matter, as the FM is the largest region of white matter while the STR is a grey matter structure.

"Our observation that immunorejection depends on the graft implantation site has important ramifications," said Dr. Walczak. "It may account for the variability in allograft survival reported by different research groups."

The accumulation of transplanted cells in the 'pocket' between two natural bordering regions of the brain, with minimal disruption to tissue integrity, appears the most plausible explanation for the dramatic difference in the allograft rejection rate, they wrote.

They concluded that the impact of the topographical and anatomical characteristics of the target site for allografting should be carefully considered when designing cell therapies for neurological disorders at both the pre-clinical and clinical levels.


'/>"/>

Contact: Robert Miranda
cogcomm@aol.com
Cell Transplantation Center of Excellence for Aging and Brain Repair
Source:Eurekalert

Related biology news :

1. Physicians belief about obesity causes impacts advice and care
2. Climate change impacts to US coasts threaten public health, safety and economy
3. New study highlights impact of environmental change on older people
4. Going trayless study shows student impact
5. Clemson University researchers to study oil and gas operations impact on Gulf Coast pelicans
6. ImpactStory metrics of academic research outreach piloted by Pensoft Publishers
7. Study looks at gray seal impact on beach water quality
8. Ozone levels have sizeable impact on worker productivity
9. Grant funded to improve mothers nutrition before pregnancy and impact on baby
10. New online tool estimates carbon and energy impact of trees
11. Missing polar weather systems could impact climate predictions
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/28/2016)... SAN FRANCISCO and BANGALORE, India ... part of EdgeVerve Systems, a product subsidiary of Infosys (NYSE: ... service provider, today announced a global partnership that ... convenient way to use mobile banking and payment services. ... Mobility is a key innovation area for financial services, but ...
(Date:4/26/2016)... BANGALORE, India and LONDON ... Infosys Finacle, part of EdgeVerve Systems, a ... ), and Onegini today announced a partnership to ... banking solutions.      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ... banks to provide their customers enhanced security to ...
(Date:4/19/2016)... , UAE, April 20, 2016 ... be implemented as a compact web-based "all-in-one" system solution ... the biometric fingerprint reader or the door interface with ... of modern access control systems. The minimal dimensions of ... ID readers into the building installations offer considerable freedom ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... , June 23, 2016   EpiBiome , a ... $1 million in debt financing from Silicon Valley Bank ... automation and to advance its drug development efforts, as ... facility. "SVB has been an incredible strategic ... services a traditional bank would provide," said Dr. ...
(Date:6/23/2016)... NEWPORT BEACH, Calif. , June 23, 2016 /PRNewswire/ ... offering new biological discoveries to the medical community, has ... and co-founder Matthew Nunez . "We ... provide us with the capital we need to meet ... funding will essentially provide us the runway to complete ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... a new line of intelligent tools designed, tuned and optimized exclusively for Okuma ... 12–17 in Chicago. The result of a collaboration among several companies with expertise ...
(Date:6/22/2016)... , June 22, 2016  According to Kalorama ... generation sequencing (NGS) market include significant efforts in ... sequencers.  More accessible and affordable sequencers, say the ... demand for consumables including sample prep materials.  The ... for Sample Preparation for Next Generation Sequencing (NGS) ...
Breaking Biology Technology: