Navigation Links
Immune cells outsmart bacterial infection by dying, Penn Vet study shows
Date:5/5/2014

A new study led by scientists at the University of Pennsylvania School of Veterinary Medicine has painted a clearer picture of the delicate arms race between the human immune system and a pathogen that seeks to infect and kill human cells.

The research explores the strategies by which the bacterial pathogen Yersinia, responsible for causing plague and gastrointestinal infections, tries to outsmart immune cell responses and looks at the tactics used by the immune system to fight back.

The senior author of the paper, which appears online this week in Proceedings of the National Academy of Sciences, is Igor E. Brodsky, an assistant professor in the Department of Pathobiology at Penn Vet. He teamed with Penn Vet's Naomi H. Philip, Annelise Snyder, Meghan Wynosky-Dolfi, Erin E. Zwack, Baofeng Hu, Louise FitzGerald and Elizabeth A. Mauldin. Alan M. Copenhaver and Sunny Shin of Penn's Perelman School of Medicine were also co-authors.

The researchers set out to try to solve a seeming paradox. They knew that one way Yersinia bacteria attempted to evade immune response was by using a protein called YopJ to block a key immune signaling pathway in host immune cells, the NF-κB pathway. Activation of this pathway by microbial infection causes infected cells to release signaling molecules, called cytokines, which mobilize the immune system to fight off infection. Yet, despite this strategic blockade, Yersinia infections do lead to recruitment of immune cells and inflammation, and the infected cells die.

In addition, previous experiments showed that a mutant version of Yersinia containing a particularly potent form of YopJ was better than the normal bacteria at killing cells in culture but paradoxically worse at causing disease in animal infections.

"So that led us to the hypothesis that maybe cell death was actually a host cell response," Brodsky said.

In other words, the researchers wondered whether the immune cells were dying on purpose, as a way to alert neighboring cells to the presence of infection.

They pursued this question by looking at molecular pathways that mediate what is known as programmed cell death. These pathways are governed in part by the activity of enzymes called caspases.

It was known that caspase-1 was important for a process known as pyroptosis, an inflammatory form of programmed cell death. This form of cell death also leads to cytokine release and inflammation. Caspase-8, on the other hand, is known to be important for apoptosis, a form of programmed cell death generally considered to be non-inflammatory.

While it would seem that the host would want to avoid cell death at any cost, certain types of death could be advantageous to the host in clearing infection. Pyroptosis, for example, is an anti-microbial strategy because, by releasing certain molecules, it gives an infected and dying host cell a way of alerting healthy neighboring cells to the presence of a bacterial intruder. Thus cell death could be the goal of Yersinia bacteria to eliminate immune cells, but it could also be a strategy of the host itself to spread the word that a pathogen is near.

While researchers knew that YopJ activity triggers both caspase-1 and caspase-8 to be processed, they didn't know how the pathways were structured and exactly what was required to lead to cell death.

By collaborating with Christopher Dillon, Patrick Fitzgerald and Douglas Green of St. Jude Children's Research Hospital and Andrew Oberst of the University of Washington, who had generated mice lacking caspase-8, the team showed that the activity of this enzyme was required for both YopJ-induced cell death and caspase-1 processing the first time that anyone has demonstrated such a link. In addition, mice lacking caspase-8 were extremely sensitive to Yersinia infection, suggesting that caspase-8 was important to host defense.

"One thing that was really surprising was that even though these mice had very high bacterial burdens, their cells were not capable of producing inflammatory cytokines," Brodsky said. "What that suggests to us is that the activation of this cell death pathway is a potential way for cells that are infected to alert uninfected cells. We think coupling of caspases-8 and -1 in this setting allows for redirection of a non-inflammatory form of cell death to an inflammatory form of cell death."

The purpose of YopJ might not be to kill the cell, the researchers believe, but instead to simply prevent the cell from producing cytokines that lead to inflammation. Yet infected host cells subvert this bacterial strategy by undergoing an inflammatory form of cell death that spreads the word that an infection is present.

Brodsky suspects that Yersinia is not alone in trying to prevent host cells from "calling for help" by blocking cytokines. He said enhancing our understanding of how signaling pathways on both the microbial and host sides operate during infection could one day lead to improved antibiotic treatments..

In the future the researchers want to understand precisely how blocking the NF-κB pathway led to caspase-8 activation, triggering the cascade that leads to cell death.


'/>"/>

Contact: Katherine Unger Baillie
kbaillie@upenn.edu
215-898-9194
University of Pennsylvania
Source:Eurekalert

Related biology news :

1. New experimental vaccine produces immune response against MERS virus
2. Low cholesterol in immune cells tied to slow progression of HIV
3. Low cholesterol in immune cells slows HIV progression
4. For an immune cell, microgravity mimics aging
5. Some immune cells defend only 1 organ
6. New method isolates immune cells for researchers to study how they ward off oral diseases
7. Sensitive balance in the immune system
8. La Jolla Institute discovers new mechanism for unleashing immune system against cancer
9. Immune cell defenders could beat invading bacteria
10. Newly discovered molecule may offer hope for immune disorders and runaway inflammation
11. IRCM researchers uncover a new function for an important player in the immune response
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/15/2016)... Research and Markets has announced ... 2016-2020,"  report to their offering.  , ... global gait biometrics market is expected to grow ... 2016-2020. Gait analysis generates multiple variables ... to compute factors that are not or cannot ...
(Date:3/31/2016)... 31, 2016   ... the "Company") LegacyXChange is excited to release ... soon to be launched online site for trading 100% ... ) will also provide potential shareholders a sense of ... to an industry that is notorious for fraud. The ...
(Date:3/23/2016)... March 23, 2016 ... Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender Anbieter ... Unternehmen mit SpeechPro zusammenarbeitet, um erstmals dessen ... wird die Möglichkeit angeboten, im Rahmen mobiler ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, ... microbial test has received AOAC Research Institute approval 061601. , “This is another ... year,” stated Bob Salter, Vice President of Regulatory and Industrial Affairs. “The Peel ...
(Date:6/23/2016)... , June, 23, 2016  The Biodesign Challenge (BDC), ... new ways to harness living systems and biotechnology, announced ... (MoMA) in New York City . ... participating students, showcased projects at MoMA,s Celeste Bartos Theater ... Antonelli , MoMA,s senior curator of architecture and design, ...
(Date:6/23/2016)... ... June 23, 2016 , ... ... launch of the Supplyframe Design Lab . Located in Pasadena, Calif., the ... future of how hardware projects are designed, built and brought to market. , ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... In a ... Hospital in Denmark detail how a patient who developed lymphedema after being treated for ... The results could change the paradigm for dealing with this debilitating, frequent side effect ...
Breaking Biology Technology: