Date:11/1/2010

es on optical sectioning, array tomography is a volumetric microscopy method based on physical serial sectioning. Ultrathin sections of tissue are cut using an ultramicrotome, and attached in order to a glass coverslip. These coverslips are then stained as desired and imaged. The resulting two-dimensional image tiles can then be reconstructed computationally into three-dimensional volume images for visualization and quantitative analysis. The thin sections allow for rapid staining and imaging and the array format allows much of the process to be automated. Stephen J. Smith and colleagues from Stanford University (http://smithlab.stanford.edu/Smithlab/Home.html) present "Array Tomography: High-Resolution Three-Dimensional Immunofluorescence," a guide to this technique that allows for visualizing previously inaccessible features of tissue structure and molecular architecture. The article is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/11/pdb.top89).

Both articles are adapted from the forthcoming Imaging: A Laboratory Manual (http://www.cshlpress.com/link/imagingp.htm). Available later this month, the collection is the cornerstone of a new laboratory manual series, designed as an essential guide for investigators who need these visualization techniques.

'/>"/>

Contact: David Crotty crotty@cshl.edu 516-422-4007 Cold Spring Harbor Laboratory Source:Eurekalert

0 GOOD

Page: 1 2
Related biology news :

1. SNM releases new fact sheet on breast cancer and molecular imaging
2. Cheskin Added Value EVP Lee Shupp Discusses Evolving Dynamics of Consumers and Imaging Tech at 6Sight
3. MU brain imaging center provides research for autism, schizophrenia and Parkinsons disease
4. Similarities in imaging the human body, Earths crust focus of conference at UH
5. UNC expands brain imaging study of infants at risk for autism
6. Studies on imaging and tracking transplanted cells
7. Fattysaurus or thinnysaurus? How dinosaurs measure up with laser imaging
8. SNM Symposium on Multimodality Cardiovascular Molecular Imaging
9. Ultrasound imaging now possible with a smartphone
10. First neuroimaging study examining motor execution in children with autism reveals new insights
11. Lyncean Technologies Inc. receives $1.2 M from NCRR to develop new imaging technique