Navigation Links
Imaging in depth: 3-dimensional microscopy featured in Cold Spring Harbor Protocols
Date:11/1/2010

COLD SPRING HARBOR, N.Y. (Mon., Nov. 1, 2010) -- Imaging has rapidly become a defining tool of the current era in biological research. But finding the right method and optimizing it for data collection can be a daunting process, even for an established imaging laboratory. Cold Spring Harbor Protocols is one of the world's leading sources for detailed technical instruction for implementation of imaging methods (http://cshprotocols.cshlp.org/cgi/collection/imaging_microscopy_general), and the November issue (http://cshprotocols.cshlp.org/TOCs/toc11_10.dtl) features articles detailing standard and cutting-edge laboratory techniques.

The confocal microscope is a workhorse of the modern life science laboratory. Its popularity stems from its ability to permit volume objects to be imaged and rendered in three dimensions. But the confocal microscope itself does not produce three-dimensional images; in fact, it only images very thin sections of a specimen that lie within its focal region. To produce a three-dimensional image, a series of thin optical sections are collected, and computer processing is used to combine them into a volumetric rendering. In "Spinning-Disk Microscopy Systems," Oxford University's Tony Wilson (http://acara.eng.ox.ac.uk/som/SOMG_home.html) reviews the many methods for producing optical sections, of which the confocal optical system is just one. He also describes a number of convenient methods of implementation that can lead to, among other things, real-time image formation. The article is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/11/pdb.top88).

While confocal microscopy relies on optical sectioning, array tomography is a volumetric microscopy method based on physical serial sectioning. Ultrathin sections of tissue are cut using an ultramicrotome, and attached in order to a glass coverslip. These coverslips are then stained as desired and imaged. The resulting two-dimensional image tiles can then be reconstructed computationally into three-dimensional volume images for visualization and quantitative analysis. The thin sections allow for rapid staining and imaging and the array format allows much of the process to be automated. Stephen J. Smith and colleagues from Stanford University (http://smithlab.stanford.edu/Smithlab/Home.html) present "Array Tomography: High-Resolution Three-Dimensional Immunofluorescence," a guide to this technique that allows for visualizing previously inaccessible features of tissue structure and molecular architecture. The article is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/11/pdb.top89).

Both articles are adapted from the forthcoming Imaging: A Laboratory Manual (http://www.cshlpress.com/link/imagingp.htm). Available later this month, the collection is the cornerstone of a new laboratory manual series, designed as an essential guide for investigators who need these visualization techniques.


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. SNM releases new fact sheet on breast cancer and molecular imaging
2. Cheskin Added Value EVP Lee Shupp Discusses Evolving Dynamics of Consumers and Imaging Tech at 6Sight
3. MU brain imaging center provides research for autism, schizophrenia and Parkinsons disease
4. Similarities in imaging the human body, Earths crust focus of conference at UH
5. UNC expands brain imaging study of infants at risk for autism
6. Studies on imaging and tracking transplanted cells
7. Fattysaurus or thinnysaurus? How dinosaurs measure up with laser imaging
8. SNM Symposium on Multimodality Cardiovascular Molecular Imaging
9. Ultrasound imaging now possible with a smartphone
10. First neuroimaging study examining motor execution in children with autism reveals new insights
11. Lyncean Technologies Inc. receives $1.2 M from NCRR to develop new imaging technique
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/29/2016)... , Nov. 29, 2016 BioDirection, a ... point-of-care products for the objective detection of concussion and ... company has successfully completed a meeting with the U.S. ... Tbit™ blood test Pre-Submission Package. During the meeting company ... system as a precursor to commencement of a planned ...
(Date:11/22/2016)... According to the new market research report "Biometric System Market ... Multi-Factor), Component (Hardware and Software), Function (Contact and Non-contact), Application, and Region ... to grow from USD 10.74 Billion in 2015 to reach USD 32.73 ... Continue Reading ... ...
(Date:11/17/2016)... announces that it has just released a new white paper authored by Zettar that ... speed data transfer storage solutions. Photo - http://photos.prnewswire.com/prnh/20161116/440463 ... ... ... Setting up a high performance computing or HPC system can be a ...
Breaking Biology News(10 mins):
(Date:12/2/2016)... , ... December 02, 2016 ... ... a consortium of pharmaceutical and biotechnology companies dedicated to collaboratively developing improved ... interested in supplying a vendor-supported, portable online UHPLC, with robust, probe-based sampling. ...
(Date:12/2/2016)... 2016   SurePure, Inc. (OTCQB: SURP) a ... Company has concluded an agreement with Tamarack Biotics under ... period to acquire units of the Company,s patented photopurification ... million.  Concurrently with the option, SurePure ... Tamarack will seek regulatory approvals in the ...
(Date:12/2/2016)... , Dec. 2, 2016 /PRNewswire/ - bioLytical Laboratories, a world leader in rapid ... Kenya,s Pharmaceutical Association members. (Photo: http://photos.prnewswire.com/prnh/20161201/444905 ) ... ... , , ... and the Kenya Pharmaceutical Association (KPA) to introduce the INSTI HIV Self Test to ...
(Date:11/30/2016)... , Nov. 30, 2016  GenomOncology today announced the appointment ... of Medical Affairs.  Dr. Coleman will oversee ... company,s proprietary knowledge-enabled platform. The GenomOncology software suite empowers molecular ... sequencing data and clinical decision support, from quality control through ... , , ...
Breaking Biology Technology: