Navigation Links
Imaging in depth: 3-dimensional microscopy featured in Cold Spring Harbor Protocols
Date:11/1/2010

COLD SPRING HARBOR, N.Y. (Mon., Nov. 1, 2010) -- Imaging has rapidly become a defining tool of the current era in biological research. But finding the right method and optimizing it for data collection can be a daunting process, even for an established imaging laboratory. Cold Spring Harbor Protocols is one of the world's leading sources for detailed technical instruction for implementation of imaging methods (http://cshprotocols.cshlp.org/cgi/collection/imaging_microscopy_general), and the November issue (http://cshprotocols.cshlp.org/TOCs/toc11_10.dtl) features articles detailing standard and cutting-edge laboratory techniques.

The confocal microscope is a workhorse of the modern life science laboratory. Its popularity stems from its ability to permit volume objects to be imaged and rendered in three dimensions. But the confocal microscope itself does not produce three-dimensional images; in fact, it only images very thin sections of a specimen that lie within its focal region. To produce a three-dimensional image, a series of thin optical sections are collected, and computer processing is used to combine them into a volumetric rendering. In "Spinning-Disk Microscopy Systems," Oxford University's Tony Wilson (http://acara.eng.ox.ac.uk/som/SOMG_home.html) reviews the many methods for producing optical sections, of which the confocal optical system is just one. He also describes a number of convenient methods of implementation that can lead to, among other things, real-time image formation. The article is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/11/pdb.top88).

While confocal microscopy reli
'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. SNM releases new fact sheet on breast cancer and molecular imaging
2. Cheskin Added Value EVP Lee Shupp Discusses Evolving Dynamics of Consumers and Imaging Tech at 6Sight
3. MU brain imaging center provides research for autism, schizophrenia and Parkinsons disease
4. Similarities in imaging the human body, Earths crust focus of conference at UH
5. UNC expands brain imaging study of infants at risk for autism
6. Studies on imaging and tracking transplanted cells
7. Fattysaurus or thinnysaurus? How dinosaurs measure up with laser imaging
8. SNM Symposium on Multimodality Cardiovascular Molecular Imaging
9. Ultrasound imaging now possible with a smartphone
10. First neuroimaging study examining motor execution in children with autism reveals new insights
11. Lyncean Technologies Inc. receives $1.2 M from NCRR to develop new imaging technique
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/9/2014)... the oils produced by algae may offer a low-cost ... optimization of cost effective strategies for large-scale algae cultivation, ... Energy has developed an innovative solution to the challenge ... in Industrial Biotechnology , a peer-reviewed journal from ... on the Industrial Biotechnology website . ...
(Date:7/9/2014)... Ill. With over 100 diseases that can attack ... top of the most wanted list? University of Illinois ... that more research is needed on the fungus that ... with cool, damp growing conditions but Macrophomina phaseolina ... and dry drought conditions. , "As the climate continues ...
(Date:7/9/2014)... a sparrow-sized fossil from China challenges the commonly held ... gained the ability to fly. The birdlike fossil is ... rather the remains of a tiny tree-climbing animal that ... Dinosaur Museum in Blanding, Utah, and Alan Feduccia of ... Springer,s Journal of Ornithology . , The fossil ...
Breaking Biology News(10 mins):Controlling contamination in open algae ponds for biofuels 2Climate change provides good growing conditions for charcoal rot in soybeans 2Climate change provides good growing conditions for charcoal rot in soybeans 3Researchers declassify dinosaurs as being the great-great-grandparents of birds 2
... An international research team led by Nanjing Agricultural ... of pear by an approach using the combination of ... only provides an invaluable new resource for breeding improvement ... on the genome evolution and other genome-wide comparative studies. ...
... 19, 2012 Medbox, Inc. (Ticker: MDBX) ( www.medboxinc.com ... they have received many inquiries from shareholders purchasing shares ... week,s volatile trading. These inquiries were responded to and ... rapport. In other news, the company ...
... person imagines moving a limb, cells in the part ... if trying to make the immobile limb work again. ... pathway between brain and muscle, the region where the ... recent years, neuroscientists and neuroengineers working in prosthetics have ...
Cached Biology News:Pear genome provides new insight into breeding improvement and evolutionary trace analysis 2Medbox Communicates with Shareholders, Comments on Pending Transition to OTC Bulletin Board, and Announces Appearance on Fox Business News Channel 2A better thought-controlled computer cursor 2A better thought-controlled computer cursor 3A better thought-controlled computer cursor 4A better thought-controlled computer cursor 5
(Date:1/14/2014)... 14, 2014 EquitiesIQ, a leading informational ... ALQA). Alliqua is an emerging biomedical company acquiring, developing, ... care market. , Free report download: http://equitiesiq.com/reports/alliqua/ ... seasoned management team and Board, which launched the company’s ...
(Date:1/14/2014)... iLab Solutions, the leader in core facility management software, ... Strategy. In this role, Michelle will work with current and ... the development of iLab products. Her main goal will be ... the scientific community by offering the most advanced, user-friendly, and ...
(Date:1/14/2014)... Global Record Systems, LLC, (GRS), a ... for patients, physicians, the biopharmaceutical industry, regulators, payers, ... signing of a three-year Research Collaboration Agreement (RCA) ... This initiative is designed to generate disruptive ...
(Date:1/14/2014)... Kerr Corporation, a leading manufacturer of innovative dental products, has ... arch impressions on its dental blog. , Titled ... serves up a list of tips to help dentists best ... David Little as he crafts a porcelain crown using Kerr’s ...
Breaking Biology Technology:EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2
... LA JOLLA, Calif., Feb. 23, 2011 ... to providing personalized treatment information to women with ... been appointed Executive Director, a new position for ... to lead the foundation as it increases its ...
... CITY, Calif., Feb. 23, 2011 Ingenuity® Systems, ... for life science researchers, today announced a research ... for next-generation sequencing (NGS) data analysis. Covance Genomics ... the drug discovery and development industry, and Ingenuity ...
... in German . , Further development of ... at reasonable costs. In the past, integration density of ... of this strategy, future components will have to reach ... Center for Functional Nanostructures (CFN) and IPCMS have now ...
Cached Biology Technology:The Clearity Foundation Hires Hillary Theakston as Executive Director 2The Clearity Foundation Hires Hillary Theakston as Executive Director 3Ingenuity Systems and Covance Collaborate to Support Next-Generation Sequencing Research 2Smallest magnetic field sensor in the world 2
... of SYBR Green I or FAM in ... fluorophores in the second channel including ... a multitude of applications such as RT-qPCR ... offers precision thermal control and a temperature ...
... identification and library manager ... that adds capabilities for ... identification of unknowns against ... of the same taxon ...
Fingerprinting II comparative quantification and polymorphism analysis is an optional module that adds comparative quantification and polymorphism analysis functions to the Fingerprinting II Basic so...
... or for use in an automated line. ... disposable manifold provides greater ease, speed, convenience, ... StatMatic II can be connected to a ... fill a microplate with one-handed operation -- ...
Biology Products: