Navigation Links
Illuminating protein networks in 1 step
Date:1/24/2010

chnology, you can potentially see everything at the same time."

Micro-western arrays adapt the technology of the micro-array, typically used to assess the expression of thousands of genes in a single experiment, to proteins. With pre-printed micro-western array gels, essentially comprising 96 miniature Western blots, scientists can compare the levels of hundreds of proteins simultaneously, or compare dozens of proteins under dozens of treatment conditions in one shot. Mere nanoliters of cell material and antibodies are needed for the experiments, reducing cost and maximizing the information obtained from a single sample.

To demonstrate the potential of the micro-western array, Jones and colleagues from the University of Chicago and the Massachusetts Institute of Technology looked at the behavior of proteins in a cancer cell line with elevated amounts of epidermal growth factor receptor (EGFR).

"We started asking questions about what we could do that no one else could previously do," Jones said. "We could actually reproducibly see 120 things at a time rather than looking at 1 or 2 or 5."

The experiments found that activating EGFR simultaneously activated several other receptors in the cell a new discovery that may explain why some tumors become resistant to cancer therapies.

With more information, the method may potentially be used clinically for more precise diagnoses of cancer and other diseases that can direct individualized treatment.

"In the clinic, you're limited by the fact that typically most cancers are diagnosed by one or two markers; you're looking for one or two markers that are high or low then trying to diagnose and treat an illness," Jones said. "Here, we can potentially measure a collection of proteins at the same time and not just focus on one guess. We've never been able do that before."

Other scientists in the field of systems biology said that micro-western arrays would make possible
'/>"/>

Contact: Robert Mitchum
robert.mitchum@uchospitals.edu
773-702-6241
University of Chicago Medical Center
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Fluorescent proteins illuminating biomedical research
2. Illuminating biology: An evolutionary perspective
3. Notre Dame study focuses on protein dynamics
4. Membrane-coat proteins: Bacteria have them too
5. Protein supplements are misused by athletes
6. Researchers identify proteins that might contribute to memory loss and Alzheimers disease
7. Carnegie Mellons Philip LeDuc discovers new protein function
8. CSHL scientists uncover role of protein critical for activating DNA replication
9. A global breakthrough in the study of a protein linked to the spread of viruses
10. Researchers pin down long-elusive protein thats essential to life as we know it
11. Unusual protein modification involved in muscular dystrophy, cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/2/2014)... considered the most ideal source of nutrition for ... role in the evolution and civilizations of human ... a large number of bacterial species, including some ... no surprise to scientists and physicians. , Indeed, ... be the result of co-evolutionary and co-adaptive interactions ...
(Date:9/2/2014)... 2014 A study published in the September ... American Academy of Child and Adolescent Psychiatry ... psychiatric diagnosis had an increased risk of developing ... Using data from the nationwide Danish registers, a ... Nielsen, Psychiatry, Aalborg University Hospital, Denmark, studied 48,299children ...
(Date:9/2/2014)... N.C. -- When you accidentally touch a hot oven, ... the basic neural circuits involved in sensing and responding ... the molecular players. , Duke researchers have made a ... involved in pain in worms, and have built a ... 2 in Nature Communications , may help direct ...
Breaking Biology News(10 mins):Are human breast milk microbiome 'neutral'? 2Are human breast milk microbiome 'neutral'? 3Risk of diabetes in children and adolescents exposed to antipsychotics 2Surprising new role for calcium in sensing pain 2Surprising new role for calcium in sensing pain 3
... Department of Community Health (MDCH) has unveiled the ... includes significant recommendations developed from medical research conducted ... Eunice Kennedy Shriver National Institute for Child Health ... at the Wayne State University School of Medicine. ...
... press release is available in German . ... great happiness among inhabitants. On those days the air is ... difficult and unhealthy. According to the latest calculations of Max ... most of world population in 2050 if no counteractive measures ...
... Butterflies learn faster when a flower is rewarding than when ... when it comes to speed of learning with rewards. These ... Kandori and Takafumi Yamaki from Kinki University in Japan. Their ... Science of Nature , is the first to investigate and ...
Cached Biology News:State of Michigan adopts NIH's PRB progesterone therapy to combat infant mortality 2State of Michigan adopts NIH's PRB progesterone therapy to combat infant mortality 3Worldwide increase of air pollution 2Rewarding work for butterflies 2
(Date:9/2/2014)... 2014 Reportbuyer.com has added a new ... Products and Global Markets https://www.reportbuyer.com/product/1923022/Human-Reproductive-Technologies-Products-and-Global-Markets.html ... The objective of the study is to present ... reproductive technologies and products. Markets are divided according ... some markets are mature, while products in other ...
(Date:9/2/2014)... NEW PROVIDENCE, N.J. , Sept. 2, ... a corporate sponsor for MassBio,s 20th Annual Golf Classic, ... MassBioEd Foundation. The event will be held on September ... Plymouth, Massachusetts . The ... Massachusetts through educational programs, workforce development, ...
(Date:9/2/2014)... Ore. -- Scientists, including University of Oregon chemist ... create scaffolds of self-assembling, synthetic proteins called peptoid ... , The accomplishment -- detailed this week in ... the Proceedings of the National Academy of ... alternative design of the two-dimensional peptoid nanosheets that ...
(Date:9/2/2014)... , Sept. 2, 2014  Technology Applications International Corporation ... its wholly-owned subsidiary Renuell Int,l, Inc. has signed an ... Space Administration ("NASA") at the Johnson Space Center in ... See photos: tapplic.com/houstonphotos/ ... very pleased to announce our latest, exclusive agreement with ...
Breaking Biology Technology:Human Reproductive Technologies: Products and Global Markets 2Human Reproductive Technologies: Products and Global Markets 3Human Reproductive Technologies: Products and Global Markets 4Human Reproductive Technologies: Products and Global Markets 5Human Reproductive Technologies: Products and Global Markets 6Human Reproductive Technologies: Products and Global Markets 7Human Reproductive Technologies: Products and Global Markets 8Human Reproductive Technologies: Products and Global Markets 9Human Reproductive Technologies: Products and Global Markets 10Human Reproductive Technologies: Products and Global Markets 11Human Reproductive Technologies: Products and Global Markets 12Human Reproductive Technologies: Products and Global Markets 13Human Reproductive Technologies: Products and Global Markets 14Human Reproductive Technologies: Products and Global Markets 15Human Reproductive Technologies: Products and Global Markets 16Human Reproductive Technologies: Products and Global Markets 17Linde helps fund STEM education in Massachusetts 2Linde helps fund STEM education in Massachusetts 3UO-Berkeley Lab unveil new nano-sized synthetic scaffolding technique 2Technology Applications International Corporation Subsidiary Enters Into Additional Exclusive Agreement with NASA at a Signing Ceremony at the Johnson Space Center In Houston, Texas 2Technology Applications International Corporation Subsidiary Enters Into Additional Exclusive Agreement with NASA at a Signing Ceremony at the Johnson Space Center In Houston, Texas 3
... dispute NimbleGen releases microarrays for DNA encyclopedia ... percent Icon Interactive lands animator as client ... managemenet Teklynx releases design software for UCC-compliant ... Benitec in contract dispute , Promega , an international ...
... In his new book The Art of the ... , Guy Kawasaki, a managing director of Garage Technology ... venture capital, most entrepreneurs would be better off spending ... relationships. , ,This is a good message, but ...
... will have a new resource to help them navigate ... related centers on campus. , ,The Office of Corporate ... , which is to include events, publications and both ... to UW=-Madison faculty, staff and students. , ,Were one ...
Cached Biology Technology:Tech Digest (11/10/2004) 2Sarcasm and rainmaking advice coexist in The Art of the Start 2Sarcasm and rainmaking advice coexist in The Art of the Start 3Sarcasm and rainmaking advice coexist in The Art of the Start 4Sarcasm and rainmaking advice coexist in The Art of the Start 5UW plans entrepreneurship program to link campus resources 2
Polyclonal Antibody to CLAN...
Request Info...
IRF7 Immunogen: Synthetic peptide corresponding to residues E(463) G T Q R E G V S S L D S S S(477) of human IRF7. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Rabbit polyclonal to TetR ( Abpromise for all tested applications). Antigen: Synthetic peptide: AQKLGVEQPTLYWHVKNKR, corresponding to amino acids 31 - 49 of TetR...
Biology Products: