Micro-western arrays adapt the technology of the micro-array, typically used to assess the expression of thousands of genes in a single experiment, to proteins. With pre-printed micro-western array gels, essentially comprising 96 miniature Western blots, scientists can compare the levels of hundreds of proteins simultaneously, or compare dozens of proteins under dozens of treatment conditions in one shot. Mere nanoliters of cell material and antibodies are needed for the experiments, reducing cost and maximizing the information obtained from a single sample.
To demonstrate the potential of the micro-western array, Jones and colleagues from the University of Chicago and the Massachusetts Institute of Technology looked at the behavior of proteins in a cancer cell line with elevated amounts of epidermal growth factor receptor (EGFR).
"We started asking questions about what we could do that no one else could previously do," Jones said. "We could actually reproducibly see 120 things at a time rather than looking at 1 or 2 or 5."
The experiments found that activating EGFR simultaneously activated several other receptors in the cell a new discovery that may explain why some tumors become resistant to cancer therapies.
With more information, the method may potentially be used clinically for more precise diagnoses of cancer and other diseases that can direct individualized treatment.
"In the clinic, you're limited by the fact that typically most cancers are diagnosed by one or two markers; you're looking for one or two markers that are high or low then trying to diagnose and treat an illness," Jones said. "Here, we can potentially measure a collection of proteins at the same time and not just focus on one guess. We've never been able do that before."
Other scientists in the field of systems biology said that micro-western arrays would make possible
|Contact: Robert Mitchum|
University of Chicago Medical Center