Navigation Links
Identifying microbial species
Date:7/3/2014

Millions of microbial species populate the world, but so far only a few have been identified due to the inability of most microbes to grow in the laboratory. Edgar Goluch, an engineer, and Slava Epstein, a biologist, aim to change this. The pair, both researchers at Northeastern University, has developed a device that allows scientists to cultivate a single species of bacteria that can then be studied and identified.

Goluch's previous research devices incorporated permeable membranes that allow sequestered bacteria to be exposed to the nutrients and molecules of their native environment. But natural competition between species, even in the wild, has so far limited the number of species of bacteria that biologists have been able to isolate with these methods and in traditional lab settings.

Goluch and Epstein's device, detailed in a paper released July 1 in the journal PLOS ONE, solves this problem. This new device permits just a single bacterial cell to enter an inner chamber containing a food source, to which the only access is a microscopic passageway just slightly narrower than a single cell. The passageway is so small that the first cell to enter it gets stuck, blocking entry by any other cell or species. Once inside, this cell pro-liferates as in previous devices, and when it does it fills up the inner chamber with a pure, single-species sample, since it is isolated from competition from other species.

In the paper, the team demonstrates the device's ability to separate mixtures of cell types in a laboratory setting. In one experiment, the researchers separated two different bacterial species whose cells are slightly different sizesE. coli and P. aueruginosa. In a second experiment, they isolated a combination of similarly sized but differently shaped species that commonly show up together in the marine environmentRoseobacter sp. and Pscyhoserpens sp. Finally, they used the device to separate cells of the same species that had been differentially tagged to glow either red or green. This final experiment validates the hypothesis that the cells grown inside the food chamber are daughters of the single cell caught in the entryway. Epstein will test the devices in the biological setting beginning this month during a research trip to Greenland.

Going forward, funding from an Instrument Development Biological Research Grant from the National Science Foundation will enable Goluch and his team of engineers to begin optimizing the device and its manufacture on a larger scale.


'/>"/>

Contact: John O'Neill
j.oneill@neu.edu
617-373-5460
Northeastern University
Source:Eurekalert  

Related biology news :

1. Beyond the microscope: Identifying specific cancers using molecular analysis
2. A new type of data papers designed to publish online interactive keys identifying biodiversity
3. Researchers develop new method for identifying lung nodules
4. UC research takes a new approach to identifying food deserts
5. Identifying a mystery channel crucial for hearing
6. DNA test better than standard screens in identifying fetal chromosome abnormalities
7. New technique for identifying gene-enhancers
8. Two researchers known for identifying and treating bubble boy disease honored by March of Dimes
9. Viral infections: Identifying the tell-tale patterns
10. Novel dark matter searches, identifying muzzle flashes, and more at 2014 APS DAMOP annual meeting
11. Childrens Research Institute finds key to identifying, enriching mesenchymal stem cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Identifying microbial species
(Date:3/27/2017)... N.Y. , March 27, 2017  Catholic ... Information and Management Systems Society (HIMSS) Analytics for ... EMR Adoption Model sm . In addition, CHS ... of U.S. hospitals using an electronic medical record ... for its high level of EMR usage in ...
(Date:3/23/2017)... The report "Gesture Recognition and Touchless Sensing Market by ... Global Forecast to 2022", published by MarketsandMarkets, the market is expected to be ... 2017 and 2022. Continue Reading ... ... ...
(Date:3/22/2017)... 2017 Optimove , provider of ... such as 1-800-Flowers and AdoreMe, today announced two ... Replenishment. Using Optimove,s machine learning algorithms, these features ... replenishment recommendations to their customers based not just ... customer intent drawn from a complex web of ...
Breaking Biology News(10 mins):
(Date:3/28/2017)... Yorba Linda, Ca (PRWEB) , ... March 28, 2017 , ... ... in various settings. The utilization of this technology is driven by its potential to ... powerful tool, there are also some challenges that must be addressed for it to ...
(Date:3/28/2017)... Michigan (PRWEB) , ... March 28, 2017 , ... ... awarded a Phase II Small Business Innovation Research (SBIR) grant from the National ... approximately $750,000 over two years to develop a suite of BioGelâ„¢ biopolymer materials ...
(Date:3/28/2017)... WASHINGTON , March 28, 2017  (AACR17, Booth ... sequencing during the American Association for Cancer Research (AACR) ... in Washington, D.C. , April 1-5, ... expression of thousands of cells at the individual level. ... Experts on-hand at AACR to discuss expanded next ...
(Date:3/28/2017)... 2017  Viking Therapeutics, Inc. ("Viking") (NASDAQ: VKTX), a ... therapies for metabolic and endocrine disorders, today announced that ... will deliver a corporate presentation at H.C. Wainwright & ... held April 3, 2017 at the St. Regis Hotel ... presentation are as follows: H.C. ...
Breaking Biology Technology: