Navigation Links
Identifying microbial species
Date:7/3/2014

Millions of microbial species populate the world, but so far only a few have been identified due to the inability of most microbes to grow in the laboratory. Edgar Goluch, an engineer, and Slava Epstein, a biologist, aim to change this. The pair, both researchers at Northeastern University, has developed a device that allows scientists to cultivate a single species of bacteria that can then be studied and identified.

Goluch's previous research devices incorporated permeable membranes that allow sequestered bacteria to be exposed to the nutrients and molecules of their native environment. But natural competition between species, even in the wild, has so far limited the number of species of bacteria that biologists have been able to isolate with these methods and in traditional lab settings.

Goluch and Epstein's device, detailed in a paper released July 1 in the journal PLOS ONE, solves this problem. This new device permits just a single bacterial cell to enter an inner chamber containing a food source, to which the only access is a microscopic passageway just slightly narrower than a single cell. The passageway is so small that the first cell to enter it gets stuck, blocking entry by any other cell or species. Once inside, this cell pro-liferates as in previous devices, and when it does it fills up the inner chamber with a pure, single-species sample, since it is isolated from competition from other species.

In the paper, the team demonstrates the device's ability to separate mixtures of cell types in a laboratory setting. In one experiment, the researchers separated two different bacterial species whose cells are slightly different sizesE. coli and P. aueruginosa. In a second experiment, they isolated a combination of similarly sized but differently shaped species that commonly show up together in the marine environmentRoseobacter sp. and Pscyhoserpens sp. Finally, they used the device to separate cells of the same species that had been differentially tagged to glow either red or green. This final experiment validates the hypothesis that the cells grown inside the food chamber are daughters of the single cell caught in the entryway. Epstein will test the devices in the biological setting beginning this month during a research trip to Greenland.

Going forward, funding from an Instrument Development Biological Research Grant from the National Science Foundation will enable Goluch and his team of engineers to begin optimizing the device and its manufacture on a larger scale.


'/>"/>

Contact: John O'Neill
j.oneill@neu.edu
617-373-5460
Northeastern University
Source:Eurekalert  

Related biology news :

1. Beyond the microscope: Identifying specific cancers using molecular analysis
2. A new type of data papers designed to publish online interactive keys identifying biodiversity
3. Researchers develop new method for identifying lung nodules
4. UC research takes a new approach to identifying food deserts
5. Identifying a mystery channel crucial for hearing
6. DNA test better than standard screens in identifying fetal chromosome abnormalities
7. New technique for identifying gene-enhancers
8. Two researchers known for identifying and treating bubble boy disease honored by March of Dimes
9. Viral infections: Identifying the tell-tale patterns
10. Novel dark matter searches, identifying muzzle flashes, and more at 2014 APS DAMOP annual meeting
11. Childrens Research Institute finds key to identifying, enriching mesenchymal stem cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Identifying microbial species
(Date:5/20/2016)... 2016  VoiceIt is excited to announce its ... By working together, VoiceIt and VoicePass will ... VoicePass take slightly different approaches to voice biometrics, ... and usability. ... partnership. "This marketing and technology partnership ...
(Date:5/9/2016)... 2016 Elevay is currently known ... freedom for high net worth professionals seeking travel for ... connected world, there is still no substitute for a ... sealing your deal with a firm handshake. This is ... advantage of citizenship via investment programs like those offered ...
(Date:4/28/2016)... First quarter 2016:   , Revenues amounted ... quarter of 2015 The gross margin was 49% (27) ... the operating margin was 40% (-13) Earnings per share ... operations was SEK 249.9 M (21.2) , Outlook   ... M. The operating margin for 2016 is estimated to ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... ... June 24, 2016 , ... While the majority of commercial spectrophotometers ... 5000 and the 6000i models are higher end machines that use the more unconventional ... spectrophotometer’s light beam from the bottom of the cuvette holder. , FireflySci has ...
(Date:6/23/2016)... CAMBRIDGE, Mass. , June 23, 2016 /PRNewswire/ ... the development of novel compounds designed to target ... compound, napabucasin, has been granted Orphan Drug Designation ... in the treatment of gastric cancer, including gastroesophageal ... cancer stemness inhibitor designed to inhibit cancer stemness ...
(Date:6/23/2016)... MA (PRWEB) , ... June 23, 2016 , ... ... Peel Plate® YM (Yeast and Mold) microbial test has received AOAC Research Institute ... platform of microbial tests introduced last year,” stated Bob Salter, Vice President of ...
(Date:6/23/2016)... ... , ... Supplyframe, the Industry Network for electronics hardware design ... Located in Pasadena, Calif., the Design Lab’s mission is to bring together inventors ... and brought to market. , The Design Lab is Supplyframe’s physical representation of ...
Breaking Biology Technology: