Navigation Links
IUPUI researchers tackle protein mechanisms behind limb regeneration
Date:12/14/2009

INDIANAPOLIS The most comprehensive study to date of the proteins in a species of salamander that can regrow appendages may provide important clues to how similar regeneration could be induced in humans.

Researchers at the School of Science at Indiana University-Purdue University Indianapolis and colleagues investigated over three hundred proteins in the amputated limbs of axolotls, a type of salamander that has the unique natural ability to regenerate appendages from any level of amputation, with the hope that this knowledge will contribute to a better understanding of the mechanisms that allow limbs to regenerate. Their findings were published online in the journal Biomedical Central Biology on November 30 (BMC Biology 7:83, 2009).

"In some ways this study of the axoltol's proteins was a fishing expedition. Fishing expedition can be a derogatory term in biology but for us it was positive, since we caught some important "fish" that enable us to formulate hypotheses as to how limb regeneration occurs," said David L. Stocum, Ph.D., professor of biology and director of the Indiana University Center for Regenerative Biology and Medicine, both in the School of Science at IUPUI, who led the study.

"Comparison of these proteins to those expressed in the amputated frog limb, which regenerates poorly, will hopefully allow us to determine how we might enhance limb regeneration in the frog and ultimately in humans, Dr. Stocum said.

With few exceptions notably the antlers of moose, deer and their close relatives, the tips of the fingers and toes of humans and rodents, and the ear tissue of certain strains of mice and rabbits the appendages of mammals do not regenerate after amputation.

Limb regeneration in the axolotl occurs when undifferentiated cells accumulate under the wound epidermis at the amputation site, a process known as the establishment of a blastema. These cells are derived by the reprogramming of differentiated cells to a less specialized state, and from resident stem cells.

"We found proteins that point to several areas that need to be studied closely to give us vital information about the mechanisms that operate to form a blastema that then goes on to regenerate the missing parts of the limb," said Dr. Stocum, an internationally respected cell and developmental biologist who has studied limb regeneration for over three decades.

Investigating the proteins found in the axolotl limb, the researchers noted three findings that appear to have significance in reprogramming cells to grow new limbs:

  1. Quantities of enzymes involved in metabolism decreased significantly during the regeneration process.
  2. There were many proteins that helped cells avoid cell death. Because amputation is very traumatic, this is critical.
  3. A protein which appears to keep cells from dividing until they are fully dedifferentiated and reprogrammed to begin forming a new limb was expressed at high levels throughout blastema formation.


'/>"/>

Contact: Cindy Fox Aisen
caisen@iupui.edu
317-274-7722
Indiana University School of Medicine
Source:Eurekalert

Related biology news :

1. IUPUI study finds living near fast food outlet not a weighty problem for kids
2. MDC researchers identify a scaffold regulating protein disposal
3. UCLA researchers engineer bacteria to turn carbon dioxide into liquid fuel
4. Berkeley Lab researchers participate in Homeland Security study of subway airflow
5. Hebrew University, American researchers show trigger to stem cell differentiation
6. Carnegie Mellon researchers receive grant
7. Researchers finds hidden sensory system in the skin
8. Researchers demonstrate nanoscale X-ray imaging of bacterial cells
9. Texas AgriLife researchers helping
10. Stopping MRSA before it becomes dangerous is possible, Sandia/UNM researchers find
11. VAI researchers find long awaited key to creating drought resistant crops
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/22/2016)... June 22, 2016 On Monday, the Department ... industry to share solutions for the Biometric Exit Program. ... and Border Protection (CBP), explains that CBP intends to ... the United States , in order ... defeat imposters. Logo - http://photos.prnewswire.com/prnh/20160622/382209LOGO ...
(Date:6/9/2016)... June 9, 2016  Perkotek an innovation leader in attendance control systems is proud ... work hours, for employers to make sure the right employees are actually signing in, ... ... ... ...
(Date:6/2/2016)... 2, 2016   The Weather Company , an IBM ... an industry-first capability in which consumers will be able to ... ask questions via voice or text and receive relevant information ... Marketers have long sought an advertising solution that ... be personal, relevant and valuable; and can scale across millions ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... June 27, 2016 , ... ... findings on what they believe could be a new and helpful biomarker for ... research. Click here to read it now. , Biomarkers are components ...
(Date:6/27/2016)... -- Liquid Biotech USA , Inc. ... Research Agreement with The University of Pennsylvania ("PENN") ... patients.  The funding will be used to assess ... outcomes in cancer patients undergoing a variety of ... to support the design of a therapeutic, decision-making ...
(Date:6/24/2016)... ... , ... While the majority of commercial spectrophotometers and fluorometers use the z-dimension ... are higher end machines that use the more unconventional z-dimension of 20mm. Z-dimension ... bottom of the cuvette holder. , FireflySci has developed several Agilent flow cell ...
(Date:6/23/2016)... , June 23, 2016   Boston ... of novel compounds designed to target cancer stemness ... has been granted Orphan Drug Designation from the ... treatment of gastric cancer, including gastroesophageal junction (GEJ) ... inhibitor designed to inhibit cancer stemness pathways by ...
Breaking Biology Technology: