Navigation Links
IU-designed probe opens new path for drug development against leading STD
Date:12/11/2013

teria to these antibiotics is on the rise.

Kuru quickly sought the collaborative support of one of the world's leading Chlamydiae researchers, Anthony Maurelli of the U.S. government's Uniformed Services University of the Health Sciences, and forwarded the new FDAA probes to Maurelli's laboratory in Bethesda, Md.

"When the FDAAs failed to label peptidoglycan in Chlamydiae, my postdoc George Liechti and I were obviously disappointed. But we really believed that Chlamydiae made peptidoglycan so we encouraged Erkin to come up with a new labeling method," Maurelli said. "We hypothesized that enzymes unique to synthesizing peptidoglycan in Chlamydiae may not have recognized the FDAA probes, so we needed Erkin to develop an alternative labeling strategy."

"It turned out the strategy Erkin needed was already in his tool box," VanNieuwenhze said. "As an alternative labeling strategy, we had synthesized a different type of probe, this time mimicking the last two amino acids of the peptidoglycan peptide sequence."

The IU team, with chemistry support from graduate student Edward Hall and postdoctoral researcher Alvin Kalinda, linked the two amino acids (D-alanine-D-alanine, or dipeptide) to chemical groups that could be selectively functionalized with fluorescent probes, after their incorporation into peptidoglycan, by a very specific chemical reaction called click chemistry. The clickable dipeptides were used and validated with a number of other bacterial species to show they were incorporated in peptidoglycan, and the new probes were forwarded to Maurelli's team.

When the new dipeptide probes were applied to cells infected with Chlamydia trachomatis, it worked immediately, said Liechti, a postdoctoral researcher in Maurelli's lab who conducted the experiments.

"The chlamydia inside the infected cells lit up with green fluorescent peptidoglycan rings in the middle of the red-stained bacterial cells," Liechti said.
'/>"/>

Contact: Stephen Chaplin
stjchap@iu.edu
812-856-1896
Indiana University
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Stanford scientists probe abandoned mine for clues about permanent CO2 sequestration
2. Light and nanoprobes detect early signs of infection
3. Light-emitting bioprobe fits in a single cell
4. Listening to cells: Scientists probe human cells with high-frequency sound
5. Mercyhurst University study probes impact of climate change on ectotherms
6. Tiny probes shine brightly to reveal the location of targeted tissues
7. Rice University researchers optimize photoluminescent probes to study DNA and more
8. Rice, MD Anderson scientists probe mystery of operon evolution
9. First antibiotic stewardship probed in the Journal of the Pediatric Infectious Diseases Society
10. Not-so-precious: Stripping gold from AFM probes allows better measurement of picoscale forces
11. The shape of things to come: NIST probes the promise of nanomanufacturing using DNA origami
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
IU-designed probe opens new path for drug development against leading STD
(Date:7/25/2014)... CORAL SPRINGS, Florida , July 25, 2014 /PRNewswire/ ... influential as more consumers are turning to digital technology ... NXT-ID, Inc. (OTCBB: NXTD), Google Inc. (NASDAQ: ... Visa Inc. (NYSE: V ), Apple Inc. ... FTNT) NXT-ID, Inc., (OTCQB: NXTD) a biometric ...
(Date:7/24/2014)... Scientists at The New York Stem Cell ... to creating a viable cell replacement therapy for ... , For the first time, NYSCF scientists generated ... samples of patients with primary progressive multiple sclerosis ... induce these stem cells into becoming oligodendrocytes, the ...
(Date:7/24/2014)... Angeles, where electric cars ply silent freeways, solar panels ... beneath the earth, from howling winds and from the ... that it is technically and economically feasible to convert ... renewable energy. Published in Energy , the plan ... energy supply in California that could create tens of ...
Breaking Biology News(10 mins):Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 2Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 3Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 4Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 5Consumer Needs for Identity Theft Security Grows with Popularity of Latest Smart Technology Products - Biometrically Secure Digital Wallet to Replace Credit Card Dependency 6NYSCF scientists 1 step closer to cell therapy for multiple sclerosis patients 2Stanford study shows how to power California with wind, water and sun 2Stanford study shows how to power California with wind, water and sun 3Stanford study shows how to power California with wind, water and sun 4
... -- Neither births nor deaths stop the flocking of organisms. ... of the University of Oregon. The notion, he says, has ... therapies. Picture any scenario in which self-propelled organisms -- ... even tiny plastic rods on a vibrating table -- move ...
... Nanoparticles have many useful applications, but also raise ... shows that plastic nanoparticles are transported through the aquatic ... full report is published Feb. 22 in the open ... to nanoparticles slowed their feeding behavior, and also affected ...
... at Buffalo engineers have developed a one-step, low-cost method ... from a single perspective, the polymer is rainbow-colored, reflecting ... filter for light, this material could form the basis ... color" of objects examined. An image of the material ...
Cached Biology News:New theory shows that neither birth nor death stops a flock 2New theory shows that neither birth nor death stops a flock 3A rainbow for the palm of your hand 2
(Date:7/25/2014)... York (PRWEB) July 25, 2014 According ... Research, "Biological Drugs Market - Global Industry Analysis, Size, ... global biological drugs market is estimated at USD 161,056.5 ... a CAGR of 10.1% from 2014 to 2020, to ... 2020. , The biological drugs market is witnessing a ...
(Date:7/25/2014)... The Conferee Networking committee is pleased ... Networking. These two-hour sessions provide a unique networking opportunity ... problems, discuss new techniques, or brainstorm new ideas in ... topic for consideration is August 31, 2014, and instructions ... Networking committee will review the topics, and status notifications ...
(Date:7/25/2014)... 25, 2014  Galmed Pharmaceuticals Ltd. (Nasdaq: ... the development and commercialization of a once-daily, oral therapy ... today announced financial results for the period ended June ... , Reported cash and cash equivalents totaling $37.4 ... December 31, 2013.  , Reported a net loss ...
(Date:7/25/2014)... At the request of the AMA, the Federal ... the public comment period (Docket No. FAA-2014-0396) for FAA’s ... by Congress as part of the FAA Modernization and ... new deadline for comments as September 23, 2014. , ... in the Federal Register on July 25, 2014, the ...
Breaking Biology Technology:Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3Pittcon 2015 Announces Call for Topics for Conferee Networking Sessions 2Galmed Pharmaceuticals Reports First Half 2014 Financial Results 2Galmed Pharmaceuticals Reports First Half 2014 Financial Results 3Galmed Pharmaceuticals Reports First Half 2014 Financial Results 4Galmed Pharmaceuticals Reports First Half 2014 Financial Results 5Galmed Pharmaceuticals Reports First Half 2014 Financial Results 6FAA Grants Comment Extension at the Request of AMA 2
... during the first quarter of 2005 amid poor overall stock ... at the larger cap life science companies. Note that these ... You may have noticed headlines at the end of last ... Cox-2 inhibitor (Bextra) off the market. , ,This stronger-than-expected action ...
badgernet badgernet badgernet ,badgernet badgernet badgernet ,badgernet badgernet badgernet ,badgernet badgernet badgernet badgernet badgernet...
... voted late Tuesday night to amend a bill granting ... newly created human embryonic stem cells would be denied. ... firms that do any research involving the destruction of ... cells from them after August 9, 2001. It ...
Cached Biology Technology:Midwest large-cap life-science sector is solid in first quarter 2Bill would deny tax credits to firms creating stem-cell cultures 2
... Plasma Immunodepletion Kit specifically removes 20 of ... or serum in preparation for further proteomics ... the plasma proteome whether you use one- ... or go straight to mass spectrometry. This ...
... a direct antibiotic-based selection ... Recombinant selection depends solely ... the need for costly ... and inexpensive process provides ...
... can perform many sophisticated DNA analysis and protein ... than just another sequence analysis product. Imagine having ... analyses to be performed each time you have ... sheets which specify exactly how your output will ...
... is the first fully automated proteomics workcell. ... operations with complete data tracking. It automates ... picking through final protein expression and measurement. ... delivering a plate to any of its ...
Biology Products: