Navigation Links
IU biologists offer clearer picture of how protein machine systems tweak gene expression
Date:3/1/2012

Indiana University biologists have found that specific types of RNA polymerase enzymes, the molecular machines that convert DNA into RNA, can differ in function based on variation in the parts -- in this case protein subunits -- used to assemble those machines.

The new findings on the synthesis and function of different RNA polymerases (Pols), including two RNA polymerases that lead author Craig Pikaard discovered over a decade ago -- the plant-specific enzymes Pol IV and Pol V -- indicate that subunit composition of the polymerases plays a role in selecting how some genes are silenced while others are not.

All eukaryotes -- a group that includes plants, animals, fungi and all other organisms with nuclei -- contain life-essential Pols I, II and III that are each built from different combinations of 12 to 17 protein subunits, with each of the three enzymes assigned specific, unique tasks in the cell. In 1999 while analyzing the newly sequenced genome of Arabidopsis thaliana, a member of the mustard family considered a model organism for experimentation in plant biology, Pikaard identified Pol IV and Pol V.

Pikaard's work has since shown that while the Pol IV and Pol V enzymes are not essential to life and are actually specialized forms of Pol II (the RNA polymerase responsible for generating RNAs that encode proteins), they play important roles in RNA-directed DNA methylation, a process that silences mobile genetic elements known as retrotransposons that can cause trouble if allowed to spread.

"In fact, most of the 12 protein subunits present in Pols II, IV and V are encoded by the same genes," Pikaard said. "Interestingly, among these common subunits are alternative forms of the ninth subunit, and the two forms of the ninth subunit (9a and 9b) are extremely similar, differing in only 8 of their 114 amino acids."

This high degree of similarity suggested 9a and 9b proteins might be redundant, but the Pikaard
'/>"/>

Contact: Steve Chaplin
stjchap@iu.edu
812-856-1896
Indiana University
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Notre Dame biologists tackling big question in evolution
2. University of Minnesota biologists replicate key evolutionary step
3. Global resource for clinical microbiologists and immunologists debuts
4. Studying bat skulls, evolutionary biologists discover how species evolve
5. The Company of Biologists: Launch of mobile web interface
6. UCLA biologists slow the aging process in fruit flies
7. Biologists use flies and mice to get to the heart of Down syndrome
8. Do bacteria age? Biologists discover the answer follows simple economics
9. American Society of Plant Biologists supports science outreach by grad students and postdocs
10. NYU biologists use Sinatra-named fly to show how to see the blues -- and the greens
11. FSU biologists fish for reasons behind endangered groupers comeback
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
IU biologists offer clearer picture of how protein machine systems tweak gene expression
(Date:12/4/2014)... PALM BEACH GARDENS, Fla. , Dec. 3, ... identity management solutions, today announced that its DigitalPersona ... 4500 fingerprint readers have been deployed throughout ... Carolina , South Carolina ... network headquartered in Southern Pines, North ...
(Date:11/21/2014)... Calif. , Nov. 20, 2014 Strict ... driver efficiency are piloting the North American and European ... of accidents growing, gesture recognition systems that are intuitive ... a mark in the industry. New ... Automotive Gesture Recognition Market in Europe ...
(Date:11/18/2014)... BETHESDA, Md. , Nov. 17, 2014 The ... European and U.S. regulatory agencies will speak and at least ... Quality Metrics Conference at the Omni Shoreham Hotel in ... is pleased once again to have significant support from the ... and Europe in our effort to ...
Breaking Biology News(10 mins):First Bank Partners with Crossmatch to Increase Log In Security 2First Bank Partners with Crossmatch to Increase Log In Security 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2
... newborn neurons in their brain memory hub excelled at ... declines with normal aging and in some anxiety disorders. ... antidepressant-like effects when combined with exercise, in the study ... Researchers, for the first time, pinpointed the effects ...
... demonstrates, for the first time, in vitro infection and ... hepatocytes.1 This study also verifies that HEV is ... animals to humans), which has been suggested for a ... epidemic and acute sporadic hepatitis in many developing countries. ...
... AWRE ), a leading supplier of technology and ... Edmund C. Reiter has resigned, effective April 1, 2011, as ... of the company citing personal reasons.   Richard ... been named co-CEOs and co-Presidents on an interim basis.  Mr. ...
Cached Biology News:Nurturing newborn neurons sharpens minds in mice 2Aware, Inc. Announces Resignation of Chief Executive Officer 2Aware, Inc. Announces Resignation of Chief Executive Officer 3
(Date:12/19/2014)... Diego, CA (PRWEB) December 18, 2014 ... a limited time special on Sartorius Biohit products ... Free on mLINE pipettes, Picus Electronic Pipette Trade-in Program, ... by Health and Safety officers around the world. They ... manual pipetting. Other features include:, ,     Full ...
(Date:12/19/2014)... NEW YORK , Dec. 18, 2014 ... novel therapies for the treatment of chronic pain, announced ... held today. "This was a transformational year ... a promising future for our company," said Sergio ... past year, we have executed on key financial, human ...
(Date:12/17/2014)... Achim Noack has been named global ... In this position, Noack will assist Jerry Stoller, CEO ... marketing strategy and supervise global marketing management and implementation ... tremendous knowledge and experience in the crop protection industry,” ... and innovative thinking will be a great asset for ...
(Date:12/17/2014)... IN (PRWEB) December 17, 2014 ... Laboratory Excellence following its most recent COLA inspection. ... by COLA, a national healthcare accreditation organization. Accreditation ... standards of quality in day-to-day operations, demonstrate continued ... pass a rigorous on-site laboratory survey. ...
Breaking Biology Technology:Major Pipette Distributor Pipette.com Announces Sartorius Year End Specials 2Relmada Therapeutics Announces Results of Annual Shareholders Meeting 2Achim Noack Joins Stoller Group as Global Vice President of Marketing and Administration 2Xeno Diagnostics Receives 3rd Award for Laboratory Excellence 2
... Corporation , a California life-science firm, announced on Monday ... Inc. , in Madison, over a patent issue. Third ... , ,The technologies involved are some of Third Wave's ... systems. Stratagene said in a release that FullVelocity allows ...
... a biotech company, raising money is a saga that never ends. ... two things: execute on the timely achievement of key milestones you ... your next financing and where and who the money will come ... for a single round whereas most VCs usually commit for two ...
...
Cached Biology Technology:Financing of biotech companies so far in 2005: A Midwest odyssey 2Financing of biotech companies so far in 2005: A Midwest odyssey 3Financing of biotech companies so far in 2005: A Midwest odyssey 4Financing of biotech companies so far in 2005: A Midwest odyssey 5
Because it is manufactured at our ISO 9001–certified facilities in Eugene, Oregon, we can guarantee that FluoroPure grade propidium iodide is greater than or equal to 98% pure by HPLC....
MOUSE ANTI RAT RT1Bu Immunogen: Rat thymocyte membrane glycoproteins...
... cloning of BAC (100+kb), fosmid (40kb), and ... stability than any othe BAC vector. On ... yields and easy recombinant DNA purification. Available ... (pSMART VC) vectors for the highest insert ...
SHEEP ANTI HUMAN ESTRONE...
Biology Products: