Navigation Links
ISU scientist helps find structure of gene-editing protein named Method of the Year
Date:1/5/2012

AMES, Iowa In the two and a half years since Adam Bogdanove, professor at Iowa State University in the Department of Plant Pathology and Microbiology, along with Matthew Moscou, a former graduate student in that department, discovered how a class of proteins from plant pathogenic bacteria find and bind specific sequences in plant genomes, researchers worldwide have moved fast to use this discovery.

Last year it was first shown that the proteins can be fused to DNA modifying enzymes to manipulate genes and gene functions by Bogdanove and colleagues at the University of Minnesota, led by former ISU professor Dan Voytas, and another group led by Iowa State University faculty member Bing Yang, professor in the Department of Genetics, Development and Cell Biology.

The fused proteins are called TAL effector nucleases, or TALENs, and can be used to better understand gene function in model plant and animal systems, to improve traits in livestock and plants, and even to treat human genetic disorders, according to Bogdanove.

The fact that these proteins can be readily engineered to bind DNA sequences of choice has resulted in a flurry of publications that demonstrate their utility in many different types of cells, including human stem cells.

Largely because of the advent of TALENs, the journal Nature Methods last month named gene editing with engineered nucleases as 2011 Method of the Year.

Now, Bogdanove and researchers from the Fred Hutchinson Cancer Research Center in Seattle have taken the next step by determining the 3-D structure of a TAL effector bound to DNA.

The findings were posted this week on Science Express, a website for early release of papers of exceptional interest that are due to be published in an upcoming issue of the journal Science.

The first author of the study is Amanda Mak, a postdoctoral researcher in the Hutchinson center. Andres Cernadas, a post doctoral resarcher in Bogdanove's lab also contributed.

By visualizing the shape of TAL effectors and how they physically interact with the DNA double helix, scientists can now better understand the biochemistry that underlies their ability to recognize and stick to specific DNA sequences.

This will in turn improve scientists' ability to target the proteins to different locations in a genome and to better predict and prevent their binding to unintended, off-target sites, according to Bogdanove.

The structure itself is also interesting from a basic biology standpoint. "It is really quite beautiful," he says, "So far there is nothing else in nature quite like it."

To determine the structure, Bogdanove collaborated with Fred Hutchinson scientists Barry Stoddard, an expert in protein DNA interactions, and Phil Bradley, a computational biologist. Led by Stoddard, the group completed the project in just over a year by using a unique combination of traditional X-ray crystallography and novel computer-based modeling methods.


'/>"/>

Contact: Adam Bogdanove
ajbog@iastate.edu
515-294-3421
Iowa State University
Source:Eurekalert

Related biology news :

1. Scripps Research Scientist wins pair of grants to study critical component of memory
2. UGA scientists hijack bacterial immune system
3. Yale scientist wins inaugural ASBMB award for her work on regulation of gene expression
4. Scripps Research scientists discover a brain cell malfunction in schizophrenia
5. Sea snails help scientists explore a possible way to enhance memory
6. Penn scientists pioneer new method for watching proteins fold
7. UC Riverside scientists release natural enemy of Asian citrus psyllid
8. Purdue scientists reveal how bacteria build homes inside healthy cells
9. Scientists identify an innate function of vitamin E
10. Kessler Foundation neuroscientist Jordan Grafman receives Humboldt Research Award
11. Chinese scientists announce the first complete sequencing of Mongolian genome
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/30/2017)... , March 30, 2017 The research ... system for three-dimensional (3D) fingerprint identification by adopting ground breaking 3D ... a new realm of speed and accuracy for use in identification, ... an affordable cost. ... ...
(Date:3/24/2017)... 2017 The Controller General of Immigration from Maldives ... Algeen have received the prestigious international IAIR Award for the most ... Reading ... Maldives ... Abdulla Algeen (small picture on the right) have received the IAIR award ...
(Date:3/22/2017)... March 21, 2017 Optimove , ... by retailers such as 1-800-Flowers and AdoreMe, today ... Recommendations and Replenishment. Using Optimove,s machine learning algorithms, ... product and replenishment recommendations to their customers based ... predictions of customer intent drawn from a complex ...
Breaking Biology News(10 mins):
(Date:7/18/2017)... ... 18, 2017 , ... G-CON today announced that it has ... its Patent Applications 14/858,857 and 13/669,785 both entitled Modular, Self-Contained, Mobile Clean Room. ... the protection of G-CON’s R&D investments and validate the G-CON platform as a ...
(Date:7/18/2017)... ... July 18, 2017 , ... Allotrope Foundation ... the first phase of the Allotrope Framework for commercial use. , The Bio-IT ... to “not only elevate the critical role of information technology in modern biomedical ...
(Date:7/17/2017)... ... 2017 , ... Whitehouse Laboratories is excited to announce that ... series of ISO 80369 standard test procedures. The ISO 80369 series of eight ... systems. With this recent expansion, Whitehouse Labs becomes one of the only facilities ...
(Date:7/17/2017)... ... July 17, 2017 , ... ... today announced safety software company AB Cube has joined its eHealth ... to advance technology innovation across life sciences and healthcare. Under the partnership, ...
Breaking Biology Technology: