Navigation Links
ISU researchers develop hybrid protein tools for gene cutting and editing
Date:8/30/2010

Ames - An Iowa State University team of researchers has developed a type of hybrid proteins that can make double-strand DNA breaks at specific sites in living cells, possibly leading to better gene replacement and gene editing therapies.

Bing Yang, assistant professor of genetics, development and cell biology, and his colleagues developed the hybrid protein by joining parts of two different bacterial proteins. One is called a TAL effector, which functions to find the specific site on the gene that needs to be cut, and the other is an enzyme called a nuclease that cuts the DNA strands.

Yang hopes the research will lead to the ability to modify genomes by cutting out defective or undesirable parts of DNA, or by replacing defective or undesirable gene segments with a functioning piece of replacement DNA - a process called homologous recombination.

Yang says that his hybrid proteins can be constructed to locate specific segments of the DNA in any type of organism.

"This breakthrough could eventually make it possible to efficiently modify plant, animal and even human genomes," said Yang. "It should be effective in a range of organisms."

The proteins work by binding onto the specific segment of DNA the researcher wants to change. Yang's proteins do this by reading the DNA sequence and finding the specific area to be cut.

Once the protein binds onto the DNA at the correct spot, the other half of Yang's protein then cuts the double-stranded DNA.

Bad or undesirable DNA can be resected (removed) and good or more desirable DNA can be introduced. When the DNA heals, the good DNA is included in the gene.

Yang started his research about a year ago after seeing the results of research by Adam Bogdanove, ISU associate professor of plant pathology, showing that TAL effectors use a very straightforward code to bind to a specific DNA sequence.

This discovery allowed Yang to predict exactly where the TAL effector nuclease will bind on
'/>"/>

Contact: Bing Yang
byang@iastate.edu
515-294-2968
Iowa State University
Source:Eurekalert  

Page: 1 2

Related biology news :

1. NC State researchers get to root of parasite genome
2. Researchers find animal with ability to survive climate change
3. Researchers find an essential gene for forming ears of corn
4. Researchers note differences between people and animals on calorie restriction
5. Researchers study acoustic communication in deep-sea fish
6. Researchers discover that growing up too fast may mean dying young in honey bees
7. Researchers study how pistachios may improve heart health
8. UI researchers find potentially toxic substance present in Chicago air
9. Researchers develop new self-training gene prediction program for fungi
10. Case Western Reserve University researchers track Chernobyl fallout
11. Childrens National researchers develop novel anti-tumor vaccine
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
ISU researchers develop hybrid protein tools for gene cutting and editing
(Date:8/28/2014)... more action is needed to ensure safeguards are in ... in PLOS ONE , researchers from Monash University, ... - combining results from different studies - to look ... areas, to determine whether they actually protect biodiversity. ... protecting an area from human exploitation made common sense, ...
(Date:8/27/2014)... Calif. , Aug. 27, 2014  WaferGen Bio-systems, ... its previously announced underwritten public offering of units of ... per unit for gross proceeds of $20 million, prior ... payable by the Company.  The shares and warrants are ... Each unit sold in the offering consists of ...
(Date:8/27/2014)... Reportlinker.com announces that a new market ... Law Enforcement Biometrics Market in North America ... About Biometrics Biometrics is a technology that ... their physical or behavioral patterns. It involves the ... as fingerprints, face recognition, DNA, palm print, hand ...
Breaking Biology News(10 mins):Protected areas proven to protect biodiversity 2WaferGen Bio-Systems Announces Successful Completion of Public Offering Raising $20 Million 2WaferGen Bio-Systems Announces Successful Completion of Public Offering Raising $20 Million 3Law Enforcement Biometrics Market in North America 2014-2018 2Law Enforcement Biometrics Market in North America 2014-2018 3Law Enforcement Biometrics Market in North America 2014-2018 4Law Enforcement Biometrics Market in North America 2014-2018 5
... increase the likelihood of immune complications following bone marrow ... November 22 advance online issue of Nature Genetics ... the donor,s genome but present in the recipient,s, transplants ... known as graft-versus-host disease, in which immune cells from ...
... and technological frontiers. The National Science Foundation has recently ... diverse efforts of the following investigators under the American ... researchers have been awarded grants for investigation of solar ... enhance the capabilities of NJIT,s Big Bear Solar Observatory ...
... to contribute their abstracts to the World Congress on ... meeting devoted to all aspects of osteoporosis and osteoarthritis ... in Florence, Italy from May 5-8, 2010. Beginning ... The submission deadline is February 4, ...
Cached Biology News:Gene mismatch influences success of bone marrow transplants 2Gene mismatch influences success of bone marrow transplants 3Gene mismatch influences success of bone marrow transplants 4NJIT receives NSF funding to improve Big Bear Telescope, study solar energy 2Special sessions announced for World Congress on Osteoporosis 2010 - IOF WCO - ECCEO10 2Special sessions announced for World Congress on Osteoporosis 2010 - IOF WCO - ECCEO10 3
(Date:8/28/2014)... Vt. (PRWEB) August 28, 2014 ... testing around the world is available from Strategic ... diagnostics companies. “ Industrial Microbiology Market Review, Fourth ... Industrial Market ” (IMMR—4) tracks and compares microbiology ... in North America, Europe and Asia, and forecasts ...
(Date:8/28/2014)... Whitehouse Laboratories is excited to announce that ... PTI Inspection Systems that will enable it to ... leak testing method development and validation programs using the ... part of this agreement, Whitehouse Labs has taken delivery ... developed and manufactured by PTI. The HVLD (high ...
(Date:8/27/2014)... August 27, 2014 In 2013, ... Lawrence Livermore National Security LLC (LLNS), ... and deliver a state-of-the-art laser system for the ... Beamlines), under construction in the Czech Republic. , ... laser system, called the " High repetition-rate Advanced ...
(Date:8/27/2014)... August 27, 2014 The Global and ... study on the current state of the Trifluoroacetic Acid ... a basic overview of the industry, including definitions, applications ... domestic market analysis are provided with a focus on ... A comparison between the international and Chinese situation is ...
Breaking Biology Technology:2 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 3Whitehouse Labs Renews Partnership with PTI Inspection Systems 2Whitehouse Labs Renews Partnership with PTI Inspection Systems 3LLNL Synchs up with ELI Beamlines on Timing System 2LLNL Synchs up with ELI Beamlines on Timing System 3Trifluoroacetic Acid Industry (Asia, Europe, North America, ROW) Analysis Report Now Available at ReportsnReports.com 2
... Advanced BioFuels Association applauded the leadership of U.S. Sen. Bill Nelson ... that extends the Investment Tax Credit to advanced biofuels.   , ... The Nelson bill, S. 3338, would ... for companies whose projects, sole and exclusive purpose is to produce advanced ...
... Calif. , May 13 SRI International announced today ... grant from the Bill & Melinda Gates Foundation.  The grant will ... Watson , Ph.D., protein biochemist in SRI,s Center for Advanced Drug ... , , ...
... ,, HARBIN, China , ... ("Renhuang" or the "Company"), a,developer, manufacturer and distributor of botanical ... successfully exhibited its products at the 63rd PHARMACHINA,fair("The Fair") held ... , , ...
Cached Biology Technology:SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 2SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 3SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 4SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 5SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 6Renhuang Pharmaceuticals Exhibits All-Natural Products at the 63rd PHARMACHINA Fair 2Renhuang Pharmaceuticals Exhibits All-Natural Products at the 63rd PHARMACHINA Fair 3Renhuang Pharmaceuticals Exhibits All-Natural Products at the 63rd PHARMACHINA Fair 4
... Reagent B - Permeabilisation medium LEUCOPERM ... suspension with Reagent A and then permeabilising ... gives antibodies access to intracellular structures and ... cells intact. Specific formulations reduce background staining ...
... Rabbit polyclonal to JNK1 (phospho T183 + Y185) ... internal domain containing phosphorylated T183/Y185. Reactivity / ... Not yet tested in other species. Background ... including the ERK, JNK, and p38 kinases. The ...
... Dako Target Retrieval Solution, pH 9 ... Tris/EDTA buffer, pH 9, intended for heat-induced ... It is well-suited for use on formalin-fixed, ... Compared with 0.01 mol/L citrate buffer, pH ...
... has been designed to provide a thin film-like barrier ... a slide. This barrier creates the proper surface ... area on the slide. PAP Pen contains a ... It can be removed, if desired, by xylene ...
Biology Products: