Navigation Links
ISU researchers develop hybrid protein tools for gene cutting and editing
Date:8/30/2010

Ames - An Iowa State University team of researchers has developed a type of hybrid proteins that can make double-strand DNA breaks at specific sites in living cells, possibly leading to better gene replacement and gene editing therapies.

Bing Yang, assistant professor of genetics, development and cell biology, and his colleagues developed the hybrid protein by joining parts of two different bacterial proteins. One is called a TAL effector, which functions to find the specific site on the gene that needs to be cut, and the other is an enzyme called a nuclease that cuts the DNA strands.

Yang hopes the research will lead to the ability to modify genomes by cutting out defective or undesirable parts of DNA, or by replacing defective or undesirable gene segments with a functioning piece of replacement DNA - a process called homologous recombination.

Yang says that his hybrid proteins can be constructed to locate specific segments of the DNA in any type of organism.

"This breakthrough could eventually make it possible to efficiently modify plant, animal and even human genomes," said Yang. "It should be effective in a range of organisms."

The proteins work by binding onto the specific segment of DNA the researcher wants to change. Yang's proteins do this by reading the DNA sequence and finding the specific area to be cut.

Once the protein binds onto the DNA at the correct spot, the other half of Yang's protein then cuts the double-stranded DNA.

Bad or undesirable DNA can be resected (removed) and good or more desirable DNA can be introduced. When the DNA heals, the good DNA is included in the gene.

Yang started his research about a year ago after seeing the results of research by Adam Bogdanove, ISU associate professor of plant pathology, showing that TAL effectors use a very straightforward code to bind to a specific DNA sequence.

This discovery allowed Yang to predict exactly where the TAL effector nuclease will bind on the DNA to make the cut.

Another study had similar results.

The concept has also been proven by Bogdanove and Dan Voytas, collaborator in genetics, development and cell biology at Iowa State, and director of the Center for Genome Engineering at the University of Minnesota, Twin Cities.

The TAL effector-nuclease approach improves on tools currently available for genome modification. It should be faster and less expensive to make TAL effector nucleases, and easier to design them to recognize specific DAN sequences, according to Yang.

Yang's findings recently appeared in the online version of the journal Nucleic Acids Research. Voytas' and Bogdanove's study also appeared recently in the journal Genetics.

Voytas and Bogdanove were also able to show that the TAL effector part of the hybrid protein can be customized to target new DNA sequences.


'/>"/>

Contact: Bing Yang
byang@iastate.edu
515-294-2968
Iowa State University
Source:Eurekalert  

Related biology news :

1. NC State researchers get to root of parasite genome
2. Researchers find animal with ability to survive climate change
3. Researchers find an essential gene for forming ears of corn
4. Researchers note differences between people and animals on calorie restriction
5. Researchers study acoustic communication in deep-sea fish
6. Researchers discover that growing up too fast may mean dying young in honey bees
7. Researchers study how pistachios may improve heart health
8. UI researchers find potentially toxic substance present in Chicago air
9. Researchers develop new self-training gene prediction program for fungi
10. Case Western Reserve University researchers track Chernobyl fallout
11. Childrens National researchers develop novel anti-tumor vaccine
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
ISU researchers develop hybrid protein tools for gene cutting and editing
(Date:6/2/2016)... -- The Department of Transport Management (DOTM) of ... US Dollar project, for the , Supply and ... and IT Infrastructure , to Decatur ... of Identity Management Solutions. Numerous renowned international vendors participated in ... was selected for the most compliant and innovative solution. ...
(Date:6/2/2016)... 2016 Perimeter Surveillance & Detection ... Physical Infrastructure, Support & Other Service  The ... offers comprehensive analysis of the global Border Security ... revenues of $17.98 billion in 2016. Now: ... leader in software and hardware technologies for advanced video ...
(Date:5/24/2016)... , May 24, 2016 Ampronix facilitates superior patient care by providing ...  3D medical LCD display is the latest premium product recently added to the range ... ... ... Sony 3d Imaging- LCD Medical Display- Ampronix News ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... 23, 2016 /PRNewswire/ - FACIT has announced the ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" or ... of a portfolio of first-in-class WDR5 inhibitors for ... as WDR5 represent an exciting class of therapies, ... medicine for cancer patients. Substantial advances have been ...
(Date:6/23/2016)... MA (PRWEB) , ... June 23, 2016 , ... ... Peel Plate® YM (Yeast and Mold) microbial test has received AOAC Research Institute ... platform of microbial tests introduced last year,” stated Bob Salter, Vice President of ...
(Date:6/23/2016)... 23, 2016   EpiBiome , a precision microbiome ... in debt financing from Silicon Valley Bank (SVB). The ... to advance its drug development efforts, as well as ... "SVB has been an incredible strategic partner to ... traditional bank would provide," said Dr. Aeron Tynes ...
(Date:6/23/2016)... ... 23, 2016 , ... In a new case report published today in STEM ... who developed lymphedema after being treated for breast cancer benefitted from an injection of ... dealing with this debilitating, frequent side effect of cancer treatment. , Lymphedema ...
Breaking Biology Technology: