Navigation Links
ISU researchers develop hybrid protein tools for gene cutting and editing
Date:8/30/2010

Ames - An Iowa State University team of researchers has developed a type of hybrid proteins that can make double-strand DNA breaks at specific sites in living cells, possibly leading to better gene replacement and gene editing therapies.

Bing Yang, assistant professor of genetics, development and cell biology, and his colleagues developed the hybrid protein by joining parts of two different bacterial proteins. One is called a TAL effector, which functions to find the specific site on the gene that needs to be cut, and the other is an enzyme called a nuclease that cuts the DNA strands.

Yang hopes the research will lead to the ability to modify genomes by cutting out defective or undesirable parts of DNA, or by replacing defective or undesirable gene segments with a functioning piece of replacement DNA - a process called homologous recombination.

Yang says that his hybrid proteins can be constructed to locate specific segments of the DNA in any type of organism.

"This breakthrough could eventually make it possible to efficiently modify plant, animal and even human genomes," said Yang. "It should be effective in a range of organisms."

The proteins work by binding onto the specific segment of DNA the researcher wants to change. Yang's proteins do this by reading the DNA sequence and finding the specific area to be cut.

Once the protein binds onto the DNA at the correct spot, the other half of Yang's protein then cuts the double-stranded DNA.

Bad or undesirable DNA can be resected (removed) and good or more desirable DNA can be introduced. When the DNA heals, the good DNA is included in the gene.

Yang started his research about a year ago after seeing the results of research by Adam Bogdanove, ISU associate professor of plant pathology, showing that TAL effectors use a very straightforward code to bind to a specific DNA sequence.

This discovery allowed Yang to predict exactly where the TAL effector nuclease will bind on
'/>"/>

Contact: Bing Yang
byang@iastate.edu
515-294-2968
Iowa State University
Source:Eurekalert  

Page: 1 2

Related biology news :

1. NC State researchers get to root of parasite genome
2. Researchers find animal with ability to survive climate change
3. Researchers find an essential gene for forming ears of corn
4. Researchers note differences between people and animals on calorie restriction
5. Researchers study acoustic communication in deep-sea fish
6. Researchers discover that growing up too fast may mean dying young in honey bees
7. Researchers study how pistachios may improve heart health
8. UI researchers find potentially toxic substance present in Chicago air
9. Researchers develop new self-training gene prediction program for fungi
10. Case Western Reserve University researchers track Chernobyl fallout
11. Childrens National researchers develop novel anti-tumor vaccine
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
ISU researchers develop hybrid protein tools for gene cutting and editing
(Date:12/10/2014)...  Wake Forest Baptist Medical Center today announced plans for ... Medicine. Funding for this $50 million capital project is ... publicly launched next summer. The medical ... series R.J. Reynolds Tobacco Company complex, adjacent to 525@vine ... with plans to be ready to welcome medical students ...
(Date:12/10/2014)... , December 9, 2014 ... BCARD Platform   ... meeting scheduling solutions for business-to-business (B2B) events, today ... leader in mobile near-field communication (NFC), Bluetooth low ...      (Logo: http://photos.prnewswire.com/prnh/20140612/691055) , Jifflenow will ...
(Date:12/4/2014)... 3, 2014  Crossmatch™, a leading provider of ... DigitalPersona ® Pro Enterprise software and U.are.U ... throughout First Bank branch locations in ... and Virginia. First Bank, a progressive community ... North Carolina , selected the Crossmatch biometrics-based ...
Breaking Biology News(10 mins):Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3First Bank Partners with Crossmatch to Increase Log In Security 2First Bank Partners with Crossmatch to Increase Log In Security 3
... QS-21A, a medicinally important molecule that helps the body ... University of Illinois at Urbana-Champaign. , In clinical trials, ... immune response in vaccine therapies against aggressive diseases such ... HIV-1 and malaria. An extract from the bark of ...
... have shown that a commonly used procedure to remove ... does not improve patient outcomes. , The procedure, called ... angioplasty in vein grafts and stenting in carotid arteries. ... the Journal of the American Medical Association, found that ...
... cells, the immune system's sentinels, patrol the labyrinthine blood ... tissue damage and demonstrate a dogged behavior not seen ... show that NKT cells crawl along vessel walls, even ... receive a chemical signal to unleash an immune-system assault ...
Cached Biology News:Columbia study shows widely used artery clearing device does not help patients during heart attack 2Natural Killers Could Lead to New Hepatitis Treatments 2Natural Killers Could Lead to New Hepatitis Treatments 3Natural Killers Could Lead to New Hepatitis Treatments 4
(Date:12/17/2014)... (PRWEB) December 17, 2014 Gene ... that it has entered into a technology access ... (ADM) to apply DNA2.0’s proprietary protein engineering technology, ... process. , “We are extremely excited that the ... platform. This proprietary bioengineering technology has now ...
(Date:12/17/2014)... SOUTH SAN FRANCISCO, Calif. , Dec. 17, ... positive results of a Phase 2 study evaluating ... for the treatment of patients with severe, chronic ... the current standard of care, including topical steroids ... endpoint was percent change in Visual Analog Scale ...
(Date:12/15/2014)...  GlassesOff Inc. (OTCBB: GLSO) announced today the appointment ... director of the Company,s Board of Directors. ... its CEO until its acquisition by Stanley Black ... Recognized as the inventor of the first Wi-Fi-based Active ... -based RFID solutions focused on improving operational efficiency, safety ...
(Date:12/13/2014)... (PRWEB) December 12, 2014 Clarassance, a ... announced its new name: Therabron Therapeutics , Inc. ... and bronchioles (a type of structure in the lungs ... company’s mission to develop novel protein therapeutics for the ... directors decided to change the name to mark the ...
Breaking Biology Technology:ADM and DNA2.0 Enter Into Protein Engineering Technology Access and Service Agreement 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 3Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 4AeroScout Founder Joins GlassesOff's Board of Directors 2AeroScout Founder Joins GlassesOff's Board of Directors 3Maryland-based Biotech Company's Path Forward in Treating Respiratory Diseases Sparks Name Change 2
... PharmAthene, Inc.,(NYSE: PIP ) a biodefense ... announced today that its financial results,for the third ... November 13,2008., PharmAthene management will be hosting ... financial results. The call is scheduled to begin ...
... New Facility in Oregon Will Help Ramp Up New Corn Hybrids Faster. ... ... announced plans to construct a parent seed corn production facility in Hermiston, ... Pioneer Hi-Bred is taking to ensure it meets rising demand for ...
... researchers are showing that Microsoft SQL ... up to 1 petabyte., REDMOND, Wash., Nov. ... its data management and business,intelligence platform, Microsoft SQL ... software to scale new heights in data warehousing,and ...
Cached Biology Technology:PharmAthene to Host Third Quarter 2008 Conference Call and Webcast on Thursday, November 13, 2008 2DuPont Expands Seed Production Capacity to Meet Growing Demand for Seed, Grain 2DuPont Expands Seed Production Capacity to Meet Growing Demand for Seed, Grain 3Could Database Software Help Cure Alzheimer's and Save the Earth? 2Could Database Software Help Cure Alzheimer's and Save the Earth? 3
... Gene silencing experiments often call for ... NeoFX Transfection Agent refines siRNA transfection ... NeoFXs lipid-based formulation can be used ... they are subcultured without increased ...
Kit for BASE20/50 dual control unit, includes mounting plate, HOLDER50T, HEATERPAD50, SRTDX temperature probe(s)....
... CHEMICON® CaspaTag™ Pan-Caspase In Situ Assay Kit ... active caspases in cells undergoing apoptosis. The ... for use in diagnostic or therapeutic procedures. ... use a novel approach to detect active ...
... nM is available for in vivo ... in: Z. Hassani, G.-F. Lemkine, P. Erbacher, ... B.-A. Demeneix. Lipid-mediated siRNA delivery down regulates ... at picomolar levels. J. Gene Med., 2005, ...
Biology Products: