Navigation Links
Human embryonic stem cell -- derived bone tissue closes massive skull injury
Date:12/2/2007

There are mice in Baltimore whose skulls were made whole again by bone tissue grown from human embryonic stem cells (hESCs).

Healing critical-size defects (defects that would not otherwise heal on their own) in intramembraneous bone, the flat bone type that forms the skull, is a vivid demonstration of new techniques devised by researchers at John Hopkins University to use hESCs for tissue regeneration.

Using mesenchymal precursor cells isolated from hESCs, the Hopkins team steered them into bone regeneration by using scaffolds, tiny, three-dimensional platforms made from biomaterials.

Physical context, it turns out, is a powerful influence on cell fate. Nathaniel S. Hwang, Jennifer Elisseeff, and colleagues at Hopkins demonstrated that by changing the scaffold materials, they could shift mesenchymal precursor cells into either of the bodys osteogenic pathways: intramembraneous, which makes skull, jaw, and clavicle bone; or endochondral, which builds the long bones and involves initial formation of cartilage, which is then transformed into bone by mineralization.

Mesenchymal precursor cells grown on an all-polymer, biodegradable scaffold followed the endochondral lineage. Those grown on a composite scaffold made of biodegradable polymers and a hard, gritty mineral called hydroxyapatite went to the intramembraneous side.

Biomaterial scaffolds provide a three-dimensional framework on which cells can proliferate and differentiate, secrete extracellular matrix, and form functional tissues, says Hwang. In addition, their known composition allowed the researchers to characterize the extracellular microenvironmental cues that drive the lineage specification.

The promise of pluripotent embryonic stem cells for regenerative medicine hangs on the development of such control techniques. Left to themselves, hESCs in culture differentiate wildly, forming a highly mixed population of cell types, which is of little use for cell-based therapy or for studying particular lineages.

Conventional hESC differentiation protocols rely on growth factors, co-culture, or genetic manipulation, say the researchers. The scaffolds offer a much more efficient method.

As a proof of principle, Hwang and colleagues seeded hESC-derived mesenchymal cells onto hydroxyapatite-composite scaffolds and used the resulting intramembraneous bone cells to successfully heal large skull defects in mice. The Hopkins researchers believe that this is the first study to demonstrate a potential application of hESC-derived mesenchymal cells in a musculoskeletal tissue regeneration application.


'/>"/>

Contact: John Fleischman
jfleischman@ascb.org
513-929-4635
American Society for Cell Biology
Source:Eurekalert

Related biology news :

1. Gene regulation, not just genes, is what sets humans apart
2. Antioxidant overload may underlie a heritable human disease
3. Facial attraction -- choice of sexual partner shaped the human face
4. Humans fostering forest-destroying disease
5. SRMs track fire retardants in humans and environment
6. St. Jude influenza survey uncovers key differences between bird flu and human flu
7. Human derived stem cells can repair rat hearts damaged by heart attack
8. Influence of sex and handedness on brain is similar in capuchin monkeys and humans
9. Gene regulation in humans is closer than expected to simple organisms
10. Pittsburgh scientists identify human source of stem cells with potential to repair muscle
11. Researchers developing device to predict proper light exposure for human health
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/6/2017)... March 6, 2017 Mintigo , ... today announced Predictive Sales Coach TM , its ... sales intelligence into Salesforce. This unique AI application ... sales organizations with deep knowledge of their customers ... intelligent engagement. Predictive Sales Coach extends Mintigo,s existing ...
(Date:3/2/2017)... 2, 2017 Who risk to be deprived ... the full report: https://www.reportbuyer.com/product/4313699/ WILL APPLE ... FIELD? Fingerprint sensors using capacitive technology represent a ... vendor Idex forecasts an increase of 360% of the ... of the fingerprint sensor market between 2014 and 2017 ...
(Date:2/28/2017)... --  Acuant , a leading provider of data capture ... new and core technologies building upon the acquisition of ... desktop Acuant FRM TM facial recognition and match ... manual review of identity documents by accredited professionals. ... most accurate capture software to streamline workflows by securely ...
Breaking Biology News(10 mins):
(Date:3/28/2017)... 28, 2017 Biostage, Inc. (Nasdaq: ... bioengineered organ implants to treat cancers and other life-threatening ... that Jim McGorry, CEO of Biostage, will ... panel at the MassBio 2017 Annual Meeting ... in Cambrige, Massachussetts. The 3D Printing and BioEngineering ...
(Date:3/28/2017)... March 28, 2017 Focus ... unpredictable sector due to the scientifically intensive operations ... medical, agricultural, environmental, and industrial. In today,s pre-market research, ... Pharmaceuticals Inc. (NASDAQ: PTLA), OvaScience Inc. (NASDAQ: OVAS), ... Inc. (NASDAQ: OCUL ). Learn more ...
(Date:3/28/2017)... (PRWEB) , ... March 28, 2017 , ... ... in the development of a new orally administered treatment for Alzheimer’s disease (AD), ... CEO John Didsbury states, “As we seek to uniquely treat the metabolic dysfunctions ...
(Date:3/27/2017)... (PRWEB) , ... March 27, 2017 , ... ... to accelerating the fight against cancer, autoimmune disease and more through a single-cell ... single-cell metabolomics and proteomics analysis platform developed in the laboratory of Dr. ...
Breaking Biology Technology: