Navigation Links
How proteins talk to each other
Date:9/21/2009

LA JOLLA, Calif., September 21, 2009 -- Investigators at Burnham Institute for Medical Research (Burnham) have identified novel cleavage sites for the enzyme caspase-3 (an enzyme that proteolytically cleaves target proteins). Using an advanced proteomic technique called N-terminomics, Guy Salvesen, Ph.D., professor and director of the Apoptosis and Cell Death Research program of Burnham's NCI-designated Cancer Center, and colleagues determined the cleavage sites on target proteins and found, contrary to previous understanding, that caspase-3 targets α-helices as well as unstructured loops. In addition, researchers found that caspase-3 and the substrates it binds to co-evolved. The paper was published on September 20 in the journal Nature Structural & Molecular Biology.

Prior to this study, scientists believed that proteases primarily cleave in unstructured loops, unstable parts of proteins that are readily accessible. The discovery that caspase-3 also cleaves α-helices contradicts a current dogma and offers new insights into protein signaling pathways.

"This was a big surprise because there shouldn't be anything for a protease to grab onto in a helix," said Dr. Salvesen. "We found that the basic concept that they don't cleave to helices is wrong. However, though we've found that proteases can cleave helices, we don't believe that's their biological function."

In addition to determining cleavage sites, the team also determined which interactions were "biologically significant." In other words which cleavages altered the function of the target protein and which ones had little impact.

The team tested the human caspase-3 and the Staphylococcal protease glutamyl endopeptidase (GluC) against the Escherichia coli (E. coli) proteosome. In a second set, the human caspase substrate was challenged with human caspase-3 . The researchers found cleavage sites using N-terminal proteomics (N-terminomics), in w
'/>"/>

Contact: Josh Baxt
jbaxt@burnham.org
858-795-5236
Burnham Institute
Source:Eurekalert

Page: 1 2

Related biology news :

1. Researchers prolong the half-life of biopharmaceutical proteins
2. When proteins change partners
3. Disrupting a destructive duo: U of T Mississauga researchers inhibit cancer proteins
4. Bionanomachines -- proteins as resistance fighters
5. Proteins in gel
6. Targeting helpers of heat shock proteins could help treat cancer, cardiovascular disease
7. TRAPping proteins that work together inside living cells
8. Pliable proteins keep photosynthesis on the light path
9. Autopilot guides proteins in brain
10. New method for detection of phosphoproteins reveals regulator of melanoma invasion
11. New high-throughput screening technique makes probing puzzling proteins possible
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/30/2014)... her eggs until they hatched 4.5 years later, according ... open-access journal PLOS ONE by Bruce Robison ... Octopuses typically have a single reproductive period during their ... their fertilized eggs until they hatch. Shallow-water octopuses typically ... but little is known about the brooding of deep-sea ...
(Date:7/30/2014)... The American Chemical Society (ACS) announced today that David ... ACS Biomaterials Science & Engineering as editor-in-chief. ... 2015, the new journal will feature high-quality research in ... engineered or naturally derived materials that interact with living ... as it reflects the tremendous growth in the field ...
(Date:7/30/2014)... potential effects of climate change on the world,s ... wrong factors, according to a new paper by ... University of Queensland, and other organizations. The authors ... missing the point when it comes to climate ... change scientists focus on the "direct" threats of ...
Breaking Biology News(10 mins):Deep-sea octopus has longest-known egg-brooding period 2ACS Biomaterials Science & Engineering: Brand-new journal launches in 2015, names editor 2Conservation scientists asking wrong questions on climate change impacts on wildlife 2
... to Bisphenol A through their mother,s diet during gestation and ... had leaner body mass than those not exposed to the ... Health have discovered. BPA is a chemical most commonly ... receipts. It once was in many hard plastic bottles, including ...
... projections of 9.5 billion people by 2050, humanity faces the ... the same amounts of water, fertilizer and arable land as ... meeting those needs by discovering a gene that could lead ... yields. The gene, called Scarecrow, is the first discovered ...
... Haven, Conn.Like organisms, cities need energy, water, and nutrients, ... in ways that are viable and sustainable over the ... model for looking systematically at the resources that flow ... out from themto understand the environmental impacts of cities ...
Cached Biology News:Female mice exposed to BPA by mothers show unexpected characteristics 2Female mice exposed to BPA by mothers show unexpected characteristics 3Newly discovered 'scarecrow' gene might trigger big boost in food production 2Urban metabolism for the urban century 2
(Date:7/30/2014)... (PRWEB) July 30, 2014 July 30, ... @APDN), a biotechnology firm that provides DNA-based authentication and ... Hogan, Ph.D. Vice President of Life Sciences, a newly ... , Dr. Hogan will lead APDN’s drive to ... relationships with biotech and healthcare customers, provide guidance to ...
(Date:7/30/2014)... , July 30, 2014  Dyadic International, ... company whose patented and proprietary technologies are used ... other proteins for the bioenergy, bio-based chemical, biopharmaceutical ... of Thomas "Tom" Dubinski as Vice President and ... leading the financial and information technology operations for ...
(Date:7/30/2014)... July 30, 2014 NCERC at ... will present his findings on corn stover pretreatment ... this week in Washington, D.C. , “Arun’s ... conference is a testament to the success of ... said. “Thanks to the foresight and vision of ...
(Date:7/30/2014)... 2014 LayerBio, Inc., an MIT spinoff ... first round of seed financing from friends and family ... to develop a novel sustained-release product for ophthalmology. , ... and their commitment to this important work," said Dr. ... one of many areas in which innovations in drug ...
Breaking Biology Technology:Dr. Michael E. Hogan, Ph.D., Joins Applied DNA Sciences as Vice President, Life Sciences 2Dr. Michael E. Hogan, Ph.D., Joins Applied DNA Sciences as Vice President, Life Sciences 3Thomas Dubinski joins Dyadic as Chief Financial Officer 2Thomas Dubinski joins Dyadic as Chief Financial Officer 3Thomas Dubinski joins Dyadic as Chief Financial Officer 4NCERC at SIUE Researcher Takes Stage at Department of Energy Conference 2NCERC at SIUE Researcher Takes Stage at Department of Energy Conference 3
... Nov. 12 As the exclusive official,breaking news service ... PR Newswire is featuring profiles of the exhibiting,organizations. The ... Company: Perry Baromedical Corporation ... Web URL : http://www.perrybaromedical.com Perry Baromedical ...
... Shawn O,Connell Discusses Implementation Issues and Recommendations, ... O,Connell, MS, RN,Director of Clinical Value Marketing, ... of Pharmacy, Clinical Services for B. Braun ... infusion therapy products and services,recently represented B. ...
... Calif., Nov. 12 TorreyPines Therapeutics,Inc. (Nasdaq: TPTX ... September 30, 2008. For the three-month period, the Company ... $5.4 million. Cash and cash,equivalents totaled $14.3 million at ... been extremely productive as we further,our transition to a ...
Cached Biology Technology:Perry Baromedical Corporation Exhibiting at MEDICA 2008 2B. Braun Directors Participate at First ISMP National Smart Pump Safety Summit 2B. Braun Directors Participate at First ISMP National Smart Pump Safety Summit 3TorreyPines Therapeutics Reports Third Quarter 2008 Results and Recent Accomplishments 2TorreyPines Therapeutics Reports Third Quarter 2008 Results and Recent Accomplishments 3TorreyPines Therapeutics Reports Third Quarter 2008 Results and Recent Accomplishments 4TorreyPines Therapeutics Reports Third Quarter 2008 Results and Recent Accomplishments 5TorreyPines Therapeutics Reports Third Quarter 2008 Results and Recent Accomplishments 6TorreyPines Therapeutics Reports Third Quarter 2008 Results and Recent Accomplishments 7
... These plastic feeding tubes for rodent ... reduce the risk of perforation and trauma. ... hassle of cleaning and the risk of ... 20ga ...
Barcoded Slides, 5 Packs of 5 Slides (25/Case)...
...
... 13(S)-HODE-d4 contains 4 deuterium atoms at the ... intended for use as an internal standard for ... spectrometry. 13(S)-HODE is produced by incubation of linoleic ... been shown to inhibit the adhesion of tumor ...
Biology Products: