Navigation Links
How good cholesterol turns bad
Date:2/21/2012

th in the United States and throughout the world. Elevated levels of LDL cholesterol and/or reduced levels of HDL cholesterol in human plasma are major risk factors for heart disease. Since CETP activity can reduce HDL-cholesterol concentrations and CETP deficiency is associated with elevated HDL-cholesterol levels, CETP inhibitors have become a highly sought-after pharmacological target for the treatment of heart disease. However, despite this intense clinical interest in CETP, little is known concerning the molecular mechanisms of CETP-mediated cholesterol transfers among lipoproteins, or even how CETP interacts with and binds to lipoproteins.

"It has been very difficult to investigate CETP mechanisms using conventional structural imaging methods because interaction with CETP can alter the size, shape and composition of lipoproteins, especially HDL," Ren says. "We were successful because we used our optimized negative-staining electron microscopy protocol that allows us to flash-fix the structure and efficiently screen more than 300 samples prepared under different conditions."

Ren and his colleagues used their optimized negative-staining electron microscopy protocol to image CETP as it interacted with spherical HDL and LDL particles. Image processing techniques yielded three-dimensional reconstructions of CETP and CETP-bound HDL. Molecular dynamic simulations were used to assess CETP molecular mobility and predict the changes that would be associated with cholesterol transfer. CETP antibodies were used to identify the CEPT interaction domains and validate the cholesterol transfer model by inhibiting CETP. This model presents inviting new targets for future CETP inhibitors.

"Our model identifies new interfaces of CETP that interact with HDL and LDL and delineates the mechanism by which the transfer of cholesterol takes place," Ren says. "This is an important step toward the rational design of next generation CETP inhibitors fo
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Cholesterol-lowering drugs and the effect on muscle repair and regeneration
2. Penn biophysicists create new model for protein-cholesterol interactions in brain and muscle tissue
3. MSU researcher studies ties between cholesterol drugs, muscle problems
4. A scientific breakthrough on the control of the bad cholesterol
5. Researchers learn that some good cholesterol isnt good enough
6. New genes present drug targets for managing cholesterol and glucose levels
7. Hypertension and cholesterol medications present in water released into the St. Lawrence River
8. Lowering your cholesterol may decrease your risk of cancer
9. Cholesterol-busting bug with a taste for waste
10. Cholesterol necessary for brain development
11. UCLA researchers reconstitute enzyme that synthesizes cholesterol drug lovastatin
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
How good cholesterol turns bad
(Date:10/16/2014)... constantly wages war on the human body. Battles ... stalemate. In pancreatic cancer, this stalemate—known as tumor ... aggressively malignant, a phenomena that is poorly understood. ... the laboratory of Salvatore Torquato, a Professor of ... conditions surrounding tumor dormancy and the switch to ...
(Date:10/16/2014)... severe respiratory tract infections and worldwide claims the ... VIB and Ghent University have succeeded in developing ... virus infection. , Xavier Saelens (VIB/UGent): "We ... way for the development of a novel approach ... suffering in numerous small children and elderly people." ...
(Date:10/15/2014)... non-traditional biophysics textbook and it describes how life functions ... discovery into biological systems and provides an understanding of ... about how our genes make proteins that control the ... in an organism. It quantifies the principles of life ... found on both large and small scales. The book ...
Breaking Biology News(10 mins):Modeling tumor dormancy 2New perspectives for development of an RSV vaccine 2New book about life as seen from physics 2
... CORAL GABLES, FL (March 14, 2012)--Convincing speeches are central to ... we select our leaders? A newly-published paper in Proceedings ... men and women vote for male and female candidates with ... found to be perceived as more competent, stronger, and more ...
... DURHAM, N.H. Researchers from the University of ... perceive changes in climate and rainfall when compared with ... to assist them to improve farming practices. "Quantifying ... of which changes matter, is fundamental to addressing the ...
... edge of the University of New Hampshire campus has contributed ... human health. The research, detailed in a recent ... , has found that one type of pavement sealcoat, common ... health and ecosystem implications. Alison Watts, research assistant professor of ...
Cached Biology News:UNH researchers find African farmers need better climate change data to improve farming practices 2UNH research adds to mounting evidence against popular pavement sealcoat 2
(Date:10/20/2014)... Calif. , Oct. 20, 2014 ... that the Company has signed a Notice of ... Regenerative Medicine (CIRM), effective October 1, 2014.  The ... Development payments and the release of additional grant ... CIRM grant award for clinical development of Asterias, ...
(Date:10/20/2014)... PureTech , a science and ... problems, announced today the closing of a $55 ... Invesco Perpetual, a $120 Billion group of funds. ... existing pipeline forward and to advance new healthcare ... creativity to really go for the big ideas ...
(Date:10/19/2014)... 19, 2014 The Latin American hardware ... in Latin America with analysis and forecast of revenue. ... and is expected to reach $2,366.8 million by 2018, ... , Browse through the TOC of the Latin American ... the in-depth analysis provided. It also provides a glimpse ...
(Date:10/19/2014)... The Asian Automatic patient billing report ... and forecast of revenue. The Automatic patient billing market ... million by 2018, at a developing CAGR of 7.2% ... of the Asian Automatic patient billing market, to get ... provides a glimpse of the segmentation of this market ...
Breaking Biology Technology:Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 2The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 3The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 2The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 3
... BioSpecifics,Technologies Corp. (OTC Bulletin Board: BSTC.OB) today announced ... stock priced at $13 per,share with aggregate proceeds ... an investment group on May 30, 2008. The ... at a premium over the market price., ...
... are confronted with potentially irreparable brain or spinal cord ... a damaged spinal cord cannot, or cannot fully, be ... connected to the K.U.Leuven have become the first to ... of injured brain tissue. The researchers have perfected a ...
... used by medical researchers may also be used in a ... water. , In a series of proof-of-concept experiments, Duke University ... target a matching portion of a gene in a common ... this new approach can be perfected, the researchers believe that ...
Cached Biology Technology:Microsurgery on the brain of the fruit fly leads to new insights into irreparable nerve injuries 2Possible new approach to purifying drinking water 2Possible new approach to purifying drinking water 3
... 3'CGGN NNNNCCG5' Inactivation Conditions: Heat inactivation at ... followed by ethanol precipitation. Storage Conditions: 10mM ... 1.0mM DTT, 0.01% BSA, 50% glycerol. Shipping ... at -20C. Unit Definition: One unit is ...
... stainless-steel shaker flask clips, purpose-designed to firmly ... All clips are equipped with a top ... include a bottom retainer spring, and silicon ... expensive glassware. Clips include the required ...
...
RABBIT ANTI HUMAN ALPHA-2-ANTIPLASMIN...
Biology Products: