Navigation Links
How cells keep in shape
Date:12/2/2007

Cells in our body come in various shapes and sizes. Each cell is shaped in such a way as to optimise it for a specific function. When things go wrong and a cell does not adopt its dedicated shape, its function can be impaired and the cell can cause problems in the body. Researchers at the European Molecular Biology Laboratory (EMBL) and the Institute for Atomic and Molecular Physics (AMOLF), The Netherlands, have now decoded a molecular mechanism that plays an important role in the development of a cells shape. In this weeks issue of Nature they report a new experimental approach that sheds light on the interaction between proteins and the cells skeleton.

That each cell type has its unique shape is due to its cytoskeleton, an internal scaffold built of protein filaments. Especially important are microtubules, dynamic filaments that constantly grow and shrink. Their spatial organisation inside cells depends on a variety of regulator proteins, some of which only interact with the growing ends of these filament. How these so called plus-end tracking proteins recognise the dynamic structure of a growing microtubule end is a long-standing puzzle. Researchers in the groups of Thomas Surrey and Damian Brunner at EMBL and of Marileen Dogetrom at AMOLF have now developed the first method that allows to simultaneously study multiple plus-end tracking molecules, so called +TIPs, in the test tube.

+TIPs specifically bind to the fast-growing plus end of a microtubule and follow it as it grows. They act as a plus-end label so that other proteins know where to bind to regulate the filaments stability, says Surrey. For years it has been impossible to reconstitute this behaviour in a test tube. Our new system now revealed which proteins need to be present for plus-end tracking and what the underlying mechanisms are.

Applying the new method they succeeded in dissecting a minimal molecular system consisting of three end tracking proteins from yeast cells. The proteins were labelled with fluorescence to monitor their behaviour with a microscope. This procedure revealed that one of the proteins has the ability to recognise the specific structure of the growing microtubule tip, binds to it and acts as a loading platform for the other two proteins. The inherent motor activity of one of the other two proteins, which allows it to walk along microtubules, contributes to the ability of the molecular system to follow growing microtubule ends selectively.

The great advantage of our new assay is that it can be applied to all kinds of other proteins that interact with microtubules, says Peter Bieling, who carried out the research in Surreys lab. It is a powerful approach that will advance our understanding of the large variety of different microtubule end tracking proteins and can shed light on their mechanics and functions.


'/>"/>

Contact: Anna-Lynn Wegener
wegener@embl.de
49-622-138-7452
European Molecular Biology Laboratory  
Source:Eurekalert

Related biology news :

1. ESF EURYI award winner aims to stop cancer cells reading their own DNA
2. Newly created cancer stem cells could aid breast cancer research
3. AIDS interferes with stem cells in the brain
4. Clemson scientists shed light on molecules in living cells
5. Social habits of cells may hold key to fighting diseases
6. UF scientists reveal how dietary restriction cleans cells
7. Human derived stem cells can repair rat hearts damaged by heart attack
8. Scientists identify embryonic stem cells by appearance alone
9. Cells united against cancer
10. Pittsburgh scientists identify human source of stem cells with potential to repair muscle
11. U of M begins nations first clinical trial using T-reg cells from cord blood in leukemia treatment
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
How cells keep in shape
(Date:3/11/2016)... http://www.apimages.com ) - --> http://www.apimages.com ) - ... ( http://www.apimages.com ) - Germany . The ... refugee identity cards. DERMALOG will be unveiling this device, and a ... next week.   --> Germany . ... new refugee identity cards. DERMALOG will be unveiling this device, and ...
(Date:3/10/2016)... India , March 10, 2016 ... a new market research report "Identity and Access Management ... & Audit, Compliance, and Governance), by Organization Size, by ... to 2020", published by MarketsandMarkets, The market is estimated ... USD 12.78 Billion by 2020, at a Compound Annual ...
(Date:3/9/2016)... 9, 2016 This BCC Research report provides ... the RNA Sequencing (RNA Seq) market for the years ... tools and reagents, data analysis, and services. ... the RNA-Sequencing market such as RNA-Sequencing tools and reagents, ... factors affecting each segment and forecast their market growth, ...
Breaking Biology News(10 mins):
(Date:5/20/2016)... ... May 20, 2016 , ... The leading Regenerative Veterinary Medicine ... experienced veterinary clients have treated over 100 of their own patients with the VetStem ... the highest level of care for their patients. , The veterinarians are Dr ...
(Date:5/20/2016)... , ... May 20, 2016 , ... The recent recall ... as reported by Food Safety News on May 12, 2016(1), demonstrates the need for ... Olsen, CEO of Baltimore-based biotech firm, PathSensors, Inc. , PathSensor’s latest ...
(Date:5/19/2016)... 2016 There is no saying ... the relentless pressures in pricing and lack in consumer ... circle though - numerous opportunities are up for grabs ... ActiveWallSt.com,s presents four names in this sector: Portola Pharmaceuticals ... (NASDAQ: VTAE ), Anthera Pharmaceuticals Inc. (NASDAQ: ...
(Date:5/19/2016)... - I dati saranno presentati ... 52 ° Congresso della Società ... - Le conclusioni dello studio indicano un tasso di ... cui il 90% presenta una d urata della risposta ... settantadue per cento dei pazienti ha riscontrato un beneficio clinico. ...
Breaking Biology Technology: