Navigation Links
How an enzyme tells stem cells which way to divide
Date:5/14/2009

Driving Miranda, a protein in fruit flies crucial to switch a stem cell's fate, is not as complex as biologists thought, according to University of Oregon biochemists. They've found that one enzyme (aPKC) stands alone and acts as a traffic cop that directs which roads daughter cells will take.

"Wherever aPKC is at on a cell's cortex or membrane, Miranda isn't," says Kenneth E. Prehoda, a professor in the chemistry department and member of the UO's Institute of Molecular Biology. When a stem cell duplicates into daughter cells, the side, or cortical domain, containing aPKC (atypical protein kinase C) continues as a stem cell, while the other domain with Miranda becomes a differentiated cell such as a neuron that forms the central nervous system.

Prehoda and co-author Scott X. Atwood, who studied in Prehoda's lab and recently earned his doctorate, describe how the mechanism works in the May 12 issue of the journal Current Biology.

Instead of a complex cascade of protein deactivation steps that many biologists have theorized, Prehoda said, aPKC strips phosphate off an energy-transfer nucleotide known as ATP and then attaches it to Miranda. This process forces Miranda away from aPKC and helps determine the fates of subsequent daughter cells.

"This process is pretty simple," he said, when viewed from a biochemical perspective. "What happens is that Miranda gets phosphorylated by aPKC, turning it into an inactivated substrate and pushing it into another location in the cell."

Much of the paper in Current Biology is devoted to why the more complex scenarios are not accurate. "There have been a lot of ideas on how this works, and most seemed to be really complicated and difficult to explain. We have found it's a much simpler mechanism," Prehoda said, adding that the mechanism likely is similar in many other types of cells, not just stem cells.

"It's a basic-research question. How does this polarity occur? In
'/>"/>

Contact: Jim Barlow
jebarlow@uoregon.edu
541-346-3481
University of Oregon
Source:Eurekalert  

Page: 1 2

Related biology news :

1. New book, Untangling the Double Helix, explores enzymes responsible for maintaining genome integrity
2. Margarita Saenz, M.D. is recipient of Genzyme/ACMGF Fellowship in Biochemical Genetics
3. A missing enzyme conveys major heart protection in pre-clinical work
4. New drug agent knocks out multiple enzymes in cancer pathway
5. Gladstone scientists reveal key enzyme in fat absorption
6. Commercial yeasts upgraded with an enzyme for biofuel production
7. Duke software dramatically speeds enzyme design
8. Gladstone scientists reveal that fat synthesizing enzyme is key to healthy skin and hair
9. Researchers find pathway and enzyme unique to tularemia organism
10. Structure of enzyme against chemical warfare agents determined
11. Disabling enzyme allows mice to gorge without becoming obese, new study finds
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
How an enzyme tells stem cells which way to divide
(Date:7/11/2014)... Research and Markets has announced ... - Industry Analysis Size Share Growth Trends and Forecast ... http://photos.prnewswire.com/prnh/20130307/600769 The rising ... need for high level security in both private and ... as username and passwords, tokens etc. are vulnerable to ...
(Date:7/11/2014)... of all vertebrates, information is transmitted through synapses, ... signal to be passed from one brain cell ... abundant type of synapse, can be either excitatory ... memory, perception and cognition, and the balance between ... For instance, every time we learn something, ...
(Date:7/10/2014)... Colo. , June 27, 2014  The ... the National Institute of Standards and Technology (NIST) ... of its academic research and forensic science expert ... The Board is an element of the NIST,s ... members are among the first appointments made to ...
Breaking Biology News(10 mins):International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019 2Blame it on the astrocytes 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 3
... Va. Researchers have unveiled the evolutionary origin ... species of Agrobacterium. A comprehensive comparison of the ... other bacteria suggests a general model for how ... are members of the Rhizobiaceae family, which also ...
... about house calls! The Wildlife Conservation Society thanks The ... campaign for their assistance in performing a brain scan ... The on-site procedureperformed by dozens of wildlife veterinarians, zookeepers, ... the Bobby Murcer Mobile MRI Unit, a 48-foot-long MRI ...
... a network of tiny sensors as small as dewdrops called ... Scattered outdoors on rocks, fence posts and doorways, or ... a completely new and cost-effective system for safeguarding and securing ... Tel Aviv University Faculty of Engineering team drew upon the ...
Cached Biology News:Evolutionary origin of bacterial chromosomes revealed 2Gorilla gets MRI at Bronx Zoo 2Gorilla gets MRI at Bronx Zoo 3Intruder alert: Tel Aviv University's 'Smart Dew' will find you! 2
(Date:7/11/2014)... , July 11, 2014 Research and ... Transfection Technology Market - Global Industry Analysis, Size, Share, ... their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... nucleic acids (either DNA or RNA) into cells. This ... of cancer cells and protein metabolism by affecting the ...
(Date:7/11/2014)... July 11, 2014 Cayenne Medical, ... specializing in the soft tissue reconstruction segment, announced ... System for surgeries involving the shoulder and extremities. ... a novel, inserter-controlled deployment method. The unique delivery ... deployment, anchor pull-out, or anchor displacement (also commonly ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Terascala, the ... announced that Alan Swahn, a former vice president of ... team as vice president of marketing. Terascala’s software when ... and NetApp create the highest performance and most reliable ... appointed to guide Terascala’s channel expansion and broaden its ...
(Date:7/10/2014)... 2014 Senior supply chain management executives ... addressed the challenges of “Reducing Cost, Lead Time, & ... strategic sourcing. Describing the partnership of the Bio Supply ... Management Institute ( SCMI) of the University of ... at the Kroc Institute of Peace and Justice in ...
Breaking Biology Technology:International Transfection Technology Market - Industry Analysis, Size, Share, Growth, Trends and Forecast to 2019 2Cayenne Medical Launches SureLock™ All-Suture Anchor System for Shoulder and Extremity Surgery 2Cayenne Medical Launches SureLock™ All-Suture Anchor System for Shoulder and Extremity Surgery 3Terascala Expands Executive Team 2Terascala Expands Executive Team 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4
... Singapore and Washington DC (July 27, 2009) According to ... Decision Making , a large-scale, covert anthrax attack on ... an extremely effective public health response, primarily because of ... response to it. The article "Predicting Hospital Surge ...
... , TARRYTOWN, N.Y., Aug. 14 /PRNewswire/ -- ... for Diabetic Patients and their Caregivers on August 13, stating that ... high (elevated) blood glucose result." Since 2006, Bayer Diabetes Care ... quinone) glucose monitoring technology for its blood glucose meters. ...
... HARBIN, China, Aug. 14 /PRNewswire-Asia-FirstCall/ -- China Sky One,Medical, ... CSKI ), a,leading fully integrated pharmaceutical company in the ... the second quarter,of 2009. , , Second ... 35.5% year-over-year to $32.2 million, -- Gross ...
Cached Biology Technology:Bioterrorism and disaster preparedness explored in special issue of Medical Decision Making 2China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 2China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 3China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 4China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 5China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 6China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 7China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 8China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 9China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 10China Sky One Medical, Inc. Announces Record Second Quarter 2009 Results 11
... In virtually every research laboratory, the ... of information accurately, inexpensively and in real ... compact lab analysis system includes the Luminex ... It utilizes xMAP technology which enables you ...
... of amino acids and peptides produced ... It is recommended for laboratory media ... in antibiotics, toxins and enzyme productions. ... pH6.8 0.5 at 25 C, 2% ...
... G2 cells were cultured in Minimum Essential Medium ... the log phase of growth. In order ... cells were fixed in acetone-methanol. The cells ... coated slide, with each wells surface specifically treated ...
... cultured in Minimum Essential Medium (Eagle) with 2 ... of growth. In order to keep the ... in acetone-methanol. The cells are arrayed on ... each wells surface specifically treated to enhance cellular ...
Biology Products: