Navigation Links
How an enzyme tells stem cells which way to divide
Date:5/14/2009

Driving Miranda, a protein in fruit flies crucial to switch a stem cell's fate, is not as complex as biologists thought, according to University of Oregon biochemists. They've found that one enzyme (aPKC) stands alone and acts as a traffic cop that directs which roads daughter cells will take.

"Wherever aPKC is at on a cell's cortex or membrane, Miranda isn't," says Kenneth E. Prehoda, a professor in the chemistry department and member of the UO's Institute of Molecular Biology. When a stem cell duplicates into daughter cells, the side, or cortical domain, containing aPKC (atypical protein kinase C) continues as a stem cell, while the other domain with Miranda becomes a differentiated cell such as a neuron that forms the central nervous system.

Prehoda and co-author Scott X. Atwood, who studied in Prehoda's lab and recently earned his doctorate, describe how the mechanism works in the May 12 issue of the journal Current Biology.

Instead of a complex cascade of protein deactivation steps that many biologists have theorized, Prehoda said, aPKC strips phosphate off an energy-transfer nucleotide known as ATP and then attaches it to Miranda. This process forces Miranda away from aPKC and helps determine the fates of subsequent daughter cells.

"This process is pretty simple," he said, when viewed from a biochemical perspective. "What happens is that Miranda gets phosphorylated by aPKC, turning it into an inactivated substrate and pushing it into another location in the cell."

Much of the paper in Current Biology is devoted to why the more complex scenarios are not accurate. "There have been a lot of ideas on how this works, and most seemed to be really complicated and difficult to explain. We have found it's a much simpler mechanism," Prehoda said, adding that the mechanism likely is similar in many other types of cells, not just stem cells.

"It's a basic-research question. How does this polarity occur? In
'/>"/>

Contact: Jim Barlow
jebarlow@uoregon.edu
541-346-3481
University of Oregon
Source:Eurekalert  

Page: 1 2

Related biology news :

1. New book, Untangling the Double Helix, explores enzymes responsible for maintaining genome integrity
2. Margarita Saenz, M.D. is recipient of Genzyme/ACMGF Fellowship in Biochemical Genetics
3. A missing enzyme conveys major heart protection in pre-clinical work
4. New drug agent knocks out multiple enzymes in cancer pathway
5. Gladstone scientists reveal key enzyme in fat absorption
6. Commercial yeasts upgraded with an enzyme for biofuel production
7. Duke software dramatically speeds enzyme design
8. Gladstone scientists reveal that fat synthesizing enzyme is key to healthy skin and hair
9. Researchers find pathway and enzyme unique to tularemia organism
10. Structure of enzyme against chemical warfare agents determined
11. Disabling enzyme allows mice to gorge without becoming obese, new study finds
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
How an enzyme tells stem cells which way to divide
(Date:12/17/2014)...  HITLAB SM announced today its completion of a ... to current U.S. Food and Drug Administration (FDA) regulations. ... and smart phone application trials utilizing the highest principles ... is determined to improve global healthcare access, quality, and ...
(Date:12/11/2014)... 2014 Research and Markets , ... has announced the addition of the "Biometrics Market ... One major trend emerging in this ... technology, it is important to upgrade biometric solutions to ...
(Date:12/10/2014)... , Dec. 9, 2014  Valencell, a leader in ... seeing a staggering demand from its licensees for highly ... revealed, is not solely coming from fitness and health ... "A wearable is only as useful as ...
Breaking Biology News(10 mins):HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2Wearable Technology Products Demand Highly Accurate Biometric Technology 2
... aren,t made of actin filaments. But their assembly is crucial ... researchers think helps to lay down memories. In the July ... ( www.jcb.org ), Rex et al. reveal that LTP,s ... different pathways, a discovery that helps explain why it is ...
... Scientists have shown that E. coli one ... remains an enigma that may hold the key to human ... Biotechnology and Biological Sciences Research Council (BBSRC) and based at the ... of the laboratory and spotted three previously unknown genes that, it ...
... televisions could be recycled and used in medicine, according to ... The chemical compound polyvinyl-alcohol (PVA) is widely used in industry ... crystal display (LCD) technology. When these sets are thrown away, ... sites. Researchers have now found a way of recovering ...
Cached Biology News:Building memories with actin 2Mystery E. coli genes essential for survival of many species 2
(Date:1/23/2015)... , Jan. 23, 2015  Array BioPharma Inc. (NASDAQ: ... reached a definitive agreement with Novartis Pharma AG to ... currently in Phase 3 development.  This agreement is conditional on ... PLC (GSK) on April 22, 2014, which are expected to ...
(Date:1/22/2015)... BOSTON , Jan. 22, 2015  Transwestern | RBJ today ... feet of office space for Shire a leading biopharmaceutical company, ... . Transwestern | RBJ,s Robert Richards , president, and ... the lease for the entire five-floor building at 95 Hayden ...
(Date:1/22/2015)... The laboratory information management systems market is a rapidly ... advancements due to factors such as rising pressure to ... systems, and increasing government support for adoption of HCIT ... in the market focus on technological advancements to further ...
(Date:1/22/2015)... January 22, 2015 zFlo Motion , ... to announce an exciting product from a new partner. ... solutions over the past 15 years, with offerings in the ... their 3D IMU system (inertial measurement unit), iSen, is opening ...
Breaking Biology Technology:Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 2Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 3Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 4Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 5Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 6Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 7Transwestern | RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 3Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 43D Motion Capture Just Got Easy 2
... Madison, Wis. - The 2006 winner of the ... first identity theft product, and has an aggressive plan that ... after two years. , ,This probably sounds like sheer fantasy ... cash flow, but consider the total market of 660 ...
... team of advisors can help an entrepreneur make a high-growth ... corporate attorney, I know that finding advisors who are a ... can be challenging for entrepreneurs. , ,In a discussion moderated ... firm Michael Best & Friedrich , I was eager ...
... to the end). The online retailing tango is in full ... the Internet changes everything. , ,We've heard this for ... of these changes (both good and bad) in the ways ... or buy and sell goods and services. For retailers and ...
Cached Biology Technology:Identity theft start up has fast-track growth plans 2Identity theft start up has fast-track growth plans 3Tapping the value of entrepreneurial advisors 2Tapping the value of entrepreneurial advisors 3Online retailing "tango" is adding some steps 2Online retailing "tango" is adding some steps 3Online retailing "tango" is adding some steps 4Online retailing "tango" is adding some steps 5Online retailing "tango" is adding some steps 6
... the most complete and professional software for ... and subsets makes it is possible to ... outputs, for example Cy3 and Cy5, standard ... different subsets of genes and/or arrays. Also ...
...
...
... Antiinflammatory, cytostatic and anti-viral agent. Inhibits several ... and tube formation of HUVEC cells. Also reduces ... expression and IL-8 release of endothelial cells. ... 18 H 16 O 8 MolWeight: ...
Biology Products: