Navigation Links
High-throughput genotyping, protein purification featured in Cold Spring Harbor Protocols
Date:11/2/2009

sequential affinity purification steps to avoid non-specific protein interactions, a common problem in identifying proteins in complexes. "Bimolecular Affinity Purification (BAP): Tandem Affinity Purification Using Two Protein Baits," from Ezra Burstein and colleagues (http://www.utsouthwestern.edu/utsw/cda/dept24482/files/536923.html), presents a variation on the TAP procedure in which the affinity moieties are placed on two different proteins of a molecular complex to isolate or detect components present in the complex. This variation, called bimolecular affinity purification (BAP), is suited for the identification of specific molecular complexes marked by the presence of two known components. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/11/pdb.prot5318).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. New high-throughput screening technique makes probing puzzling proteins possible
2. NIH grants Phylonix Phase II SBIR to develop high-throughput in vivo zebrafish assays
3. Moderate amounts of protein per meal found best for building muscle
4. Chemosensitivity of cancer cells depends on their protein dependency
5. Biochemical on-switch could solve protein purification challenge
6. Single-stranded DNA-binding protein is dynamic, critical to DNA repair
7. University of the Basque Country study on proteins related to Alzheimers
8. InVivo and CEVEC pharmaceuticals sign license agreement regarding the use of human CAP-T Technology for production of recombinant proteins
9. How the 100th protein structure solved at Diamond impacts our understanding of how insects smell
10. All tied up: Tethered protein provides long-sought answer
11. How proteins talk to each other
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/10/2014)... Pa. , July 2, 2014  Unisys Corporation,s (NYSE: ... , Unisys Nederland N.V., today announced that it has ... deliver, implement and manage a new Basic Provision Biometrics solution ... Unisys won the contract with a solution based ... management software. The term of the contract is up to ...
(Date:7/10/2014)... SHELTON, Conn. , July 2, 2014 NXT-ID, Inc., ... market, reports that CEO Mr. Gino Pereira was ... radio show in Tampa Florida . Mr. ... radio host Cedric Harris and how the Wocket™ ... secure alternative.  Cedric Harris tells ...
(Date:7/10/2014)... 3, 2014 Research and Markets ( ... "Global Gesture Recognition & Touch-Less Sensing (2D, ... 2020" report to their offering ... Recognition & Touch-Less Sensing Market to Grow Exponentially. ... while, but the companies were unable to leverage ...
Breaking Biology News(10 mins):Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 2Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 4Global Gesture Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, IR, Capacitive) Market - Forecasts to 2020 2
... The National Research Council Canada (NRC) recently helped Olympus, ... imaging, to design and commercialize a CARS (Coherent Anti-stokes ... open its doors to Canadian researchers and the medical ... The new Olympus microscope is based on a femtosecond ...
... the Falkland Islands during the voyage of the Beagle in 1835, ... and correctly commented that it was being hunted in such large ... Darwin was baffled by how this animal got on the islands, ... evolution by natural selection. Now, UCLA biologists ...
... This release is available in German . ... who factor the planet,s multi-trillion dollar ecosystem services into their ... higher rates of return and stronger economic growth in the ... countries have already made the link to a limited extent ...
Cached Biology News:Small, beautiful and additive-free 2Funny, you don't look related 2Funny, you don't look related 3Funny, you don't look related 4TEEB report released on the economics of ecosystems and biodiversity for policy makers 2TEEB report released on the economics of ecosystems and biodiversity for policy makers 3TEEB report released on the economics of ecosystems and biodiversity for policy makers 4TEEB report released on the economics of ecosystems and biodiversity for policy makers 5
(Date:7/12/2014)... Albany, New York (PRWEB) July 12, 2014 ... market analysis for various reagents used in ... biotechnology is driving the life science reagents ... this study include manufacturers and providers of ... Science Reagents Market Report: http://www.transparencymarketresearch.com/life-sciences-reagents-analytical-reagents.html . ...
(Date:7/11/2014)... -- EPFL and the University of  Geneva ... John P. Donoghue . The American neuroscientist is to ... The new Wyss Center for Bio- and ... , has named as its director one of ... , founder of Brown University,s Institute for Brain Science, ...
(Date:7/10/2014)... Product and process impurities in ... and are often “lost in the noise,” making ... presenters Dr. Rowel Tobias, Senior Scientist, Protein Chemistry ... of Quality at Nanotherapeutics, Inc., to lear about ... detection and quantitation while achieving the required specificity ...
(Date:7/10/2014)... Poway, CA (PRWEB) July 10, 2014 Robert ... Regenerative Veterinary Medicine company, is proud to announce the relaunch ... Pets, launching with a new series called “ What are ... to give an honest and straightforward foundation in the basics ... an educated decision on the right type of treatment when ...
Breaking Biology Technology:Transparency Market Research: Life Science Reagents Market- PCR Reagent Kits, Cell and Tissue Culture, Flow Cytometry, Electrophoresis and Chromatography 2Transparency Market Research: Life Science Reagents Market- PCR Reagent Kits, Cell and Tissue Culture, Flow Cytometry, Electrophoresis and Chromatography 3John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 2John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 3Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3
... , ... (Nasdaq: CBPO ) ("China Biologic," or the "Company"), one of ... management team will ring the opening bell at the,NASDAQ MarketSite in ... , China Biologic,s common stock began trading ...
... , BEIJING, ... LTUS) ("Lotus" or the "Company") a growing developer and,producer ... in the People,s Republic of China ("PRC"), today reported ... entity of Lotus,appointed Mr. Jinzhong HAN to be General ...
... ... that it has entered into a multi-year, sole-source supply agreement with leading global dairy ... formulas and milk-based Anmum™ Materna products for pregnant and nursing mothers. , ... Columbia, MD (Vocus) December 10, ...
Cached Biology Technology:China Biologic Products to Ring NASDAQ Opening Bell 2China Biologic Products to Ring NASDAQ Opening Bell 3Lotus Pharmaceuticals, Inc. Appoints OTC Division Head 2Lotus Pharmaceuticals, Inc. Appoints OTC Division Head 3Martek Signs Sole-Source Supply Agreement with Fonterra 2Martek Signs Sole-Source Supply Agreement with Fonterra 3
... The user-friendly Eppendorf Thermomixer R offers ... that require shaking, heating, and cooling. ... expanded its application and temperature control ... each accommodate 24 micro test tubes, ...
... is a high quality microarray scanner based on ... rejection and spatial resolution. The DNAscope LM ... cost microarray scanner that is upgradeable to a ... Features: Includes control module (computer), ...
... for arraying proteins of interest with ... equipment. DiscoverLight Protein Array Kits allow ... cell lysate or antibody in a ... specially designed nitrocellulose membranes with a ...
... Conjugate Stabilizer is an aqueous solution ... and other non-toxic stabilizing chemicals in ... 5.5 6.5. This product ... and 0.02% bromonitrodioxane as a preservative. ...
Biology Products: