Navigation Links
High-throughput genotyping, protein purification featured in Cold Spring Harbor Protocols
Date:11/2/2009

COLD SPRING HARBOR, N.Y. (Mon., Nov. 2, 2009) -- The introduction of high-throughput laboratory methods has greatly increased the pace of research into the genetics of complex diseases. Instead of focusing only on one or a few coding variants in a small sample of individuals, the ability to accurately and efficiently genotype many individuals and to cover more of the variation within individual genes has resulted in genetic studies with greater statistical power. "Laboratory Methods for High-Throughput Genotyping," from Howard Edenberg and Yunlong Liu at the University of Indiana (http://cmg.iupui.edu/EdenbergLab/ and http://compbio.iupui.edu/people/details/10), presents an overview of the commonly used methods for high-throughput single-nucleotide polymorphism (SNP) genotyping for different stages of genetic studies and briefly reviews some of the high-throughput sequencing methods just coming into use. The authors also discuss recent developments in "next-generation" sequencing that will enable other kinds of studies. The article is excerpted from the recently published Genetics of Complex Human Diseases laboratory manual (http://www.cshlpress.com/link/gchdp.htm). It is featured in the November issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc11_09.dtl) and is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2009/11/pdb.top62).

The tandem affinity purification (TAP) procedure was pioneered in yeast for the purpose of purifying and characterizing protein complexes and has since been adapted for use in many organisms, including mammalian systems. The TAP procedure involves two sequential affinity purification steps to avoid non-specific protein interactions, a common problem in identifying proteins in complexes. "Bimolecular Affinity Purification (BAP): Tandem Affinity Purification Using Two Protein Baits," from Ezra Burstein and colleagues (http://www.utsouthwestern.edu/utsw/cda/dept24482/files/536923.html), presents a variation on the TAP procedure in which the affinity moieties are placed on two different proteins of a molecular complex to isolate or detect components present in the complex. This variation, called bimolecular affinity purification (BAP), is suited for the identification of specific molecular complexes marked by the presence of two known components. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/11/pdb.prot5318).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. New high-throughput screening technique makes probing puzzling proteins possible
2. NIH grants Phylonix Phase II SBIR to develop high-throughput in vivo zebrafish assays
3. Moderate amounts of protein per meal found best for building muscle
4. Chemosensitivity of cancer cells depends on their protein dependency
5. Biochemical on-switch could solve protein purification challenge
6. Single-stranded DNA-binding protein is dynamic, critical to DNA repair
7. University of the Basque Country study on proteins related to Alzheimers
8. InVivo and CEVEC pharmaceuticals sign license agreement regarding the use of human CAP-T Technology for production of recombinant proteins
9. How the 100th protein structure solved at Diamond impacts our understanding of how insects smell
10. All tied up: Tethered protein provides long-sought answer
11. How proteins talk to each other
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/6/2016)... DALLAS , Dec. 6, 2016 ... criminal justice technology solutions for public safety, investigation, ... (PEP) jointly announced today a five (5) year ... exclusive agreement to expand the rehabilitation and reentry ... PEP History Established in 2004, the Prison ...
(Date:12/2/2016)... 2016   SoftServe , a global digital ... an electrocardiogram (ECG) biosensor analysis system for continuous ... asset. The smart system ensures device-to-device communication between ... and mobile devices to easily ,recognize, and monitor ... vehicle technology advances, so too must the security ...
(Date:11/29/2016)... , Nov. 29, 2016 BioDirection, a privately ... products for the objective detection of concussion and other ... has successfully completed a meeting with the U.S. Food ... blood test Pre-Submission Package. During the meeting company representatives ... as a precursor to commencement of a planned pilot ...
Breaking Biology News(10 mins):
(Date:12/8/2016)... , December 8, 2016 ... Unternehmen für Molekulargenetik, erweitert seine Palette an anpassbaren ... NGS Custom FH Panels, das ein schnelles und ... ermöglicht. Das Panel bietet eine Erkennung von Einzel-Nukleotid-Variationen ... (CNV) mit einem einzigen kleinen Panel und ermöglicht ...
(Date:12/8/2016)... ... December 08, 2016 , ... ... In response to client demand KbioBox developed a sophisticated “3 click” gene dditing ... are accessible from KBioBox’s new website, https://www.kbiobox.com/ and powered by ...
(Date:12/8/2016)... Iowa (PRWEB) , ... December 08, 2016 , ... This ... asynchronous approvals for biotech crops. The authors focus on the economic effects in countries ... global approval of new biotech crops and the resultant risk of low level presence ...
(Date:12/8/2016)... Fla. , Dec. 8, 2016  HedgePath ... company that discovers, develops and plans to commercialize ... its shares of common stock were approved for ... will begin trading on the OTCQX, effective today, ... qualify for the OTCQX market, companies must meet ...
Breaking Biology Technology: