Navigation Links
High-throughput genotyping, protein purification featured in Cold Spring Harbor Protocols
Date:11/2/2009

COLD SPRING HARBOR, N.Y. (Mon., Nov. 2, 2009) -- The introduction of high-throughput laboratory methods has greatly increased the pace of research into the genetics of complex diseases. Instead of focusing only on one or a few coding variants in a small sample of individuals, the ability to accurately and efficiently genotype many individuals and to cover more of the variation within individual genes has resulted in genetic studies with greater statistical power. "Laboratory Methods for High-Throughput Genotyping," from Howard Edenberg and Yunlong Liu at the University of Indiana (http://cmg.iupui.edu/EdenbergLab/ and http://compbio.iupui.edu/people/details/10), presents an overview of the commonly used methods for high-throughput single-nucleotide polymorphism (SNP) genotyping for different stages of genetic studies and briefly reviews some of the high-throughput sequencing methods just coming into use. The authors also discuss recent developments in "next-generation" sequencing that will enable other kinds of studies. The article is excerpted from the recently published Genetics of Complex Human Diseases laboratory manual (http://www.cshlpress.com/link/gchdp.htm). It is featured in the November issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc11_09.dtl) and is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2009/11/pdb.top62).

The tandem affinity purification (TAP) procedure was pioneered in yeast for the purpose of purifying and characterizing protein complexes and has since been adapted for use in many organisms, including mammalian systems. The TAP procedure involves two sequential affinity purification steps to avoid non-specific protein interactions, a common problem in identifying proteins in complexes. "Bimolecular Affinity Purification (BAP): Tandem Affinity Purification Using Two Protein Baits," from Ezra Burstein and colleagues (http://www.utsouthwestern.edu/utsw/cda/dept24482/files/536923.html), presents a variation on the TAP procedure in which the affinity moieties are placed on two different proteins of a molecular complex to isolate or detect components present in the complex. This variation, called bimolecular affinity purification (BAP), is suited for the identification of specific molecular complexes marked by the presence of two known components. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/11/pdb.prot5318).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. New high-throughput screening technique makes probing puzzling proteins possible
2. NIH grants Phylonix Phase II SBIR to develop high-throughput in vivo zebrafish assays
3. Moderate amounts of protein per meal found best for building muscle
4. Chemosensitivity of cancer cells depends on their protein dependency
5. Biochemical on-switch could solve protein purification challenge
6. Single-stranded DNA-binding protein is dynamic, critical to DNA repair
7. University of the Basque Country study on proteins related to Alzheimers
8. InVivo and CEVEC pharmaceuticals sign license agreement regarding the use of human CAP-T Technology for production of recombinant proteins
9. How the 100th protein structure solved at Diamond impacts our understanding of how insects smell
10. All tied up: Tethered protein provides long-sought answer
11. How proteins talk to each other
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/22/2017)... -- Vigilant Solutions , a vehicle location and ... today the appointment of retired FBI special agent ... development. Mr. Sheridan brings more than 21 ... on the aviation transportation sector, to his new role ... served as the Aviation Liaison Agent Coordinator (ALAC) in ...
(Date:3/20/2017)... 2017 PMD Healthcare announces the release of ... Management System (WMS), a remote, real-time lung health monitoring ... Healthcare is a Medical Device, Digital Health, and Chronic ... creating innovative solutions that empower people to improve their ... PMD developed the first ever personal spirometer, Spiro PD, ...
(Date:3/13/2017)... Future of security: Biometric Face Matching software  Continue ... ... to match face pictures against each other or against large databases. The recognition ... ... software for biometric Face Matching on the market. The speed is at 100 ...
Breaking Biology News(10 mins):
(Date:6/28/2017)... ... ... Supplies of the critical medical isotope molybdenum-99 (Mo-99) are secure and reliable ... and Molecular Imaging (SNMMI) 2017 annual meeting in Denver, Colorado in June. Mo-99 ... million nuclear medicine procedures worldwide every year. (1) , Sally Schwarz, President of ...
(Date:6/27/2017)... ... June 27, 2017 , ... Brain State Technologies, a ... campaign on June 15th to fund production of the new B2v2 wearable brainwave ... Kickstarter goal by more than 150% in a little over a week. , ...
(Date:6/27/2017)... ... June 27, 2017 , ... Indiana-based Xylogenics ... yeast production and fermentation process. The efficiencies created by the newest strain ... most notably the ethanol industry wherein individual production plants are planning to invest ...
(Date:6/26/2017)... ... 2017 , ... Third Wave Bioactives, LLC announces the addition of Brett Thompson. ... business development and ensuring quality customer experience. , Brett brings to Third ... technical, marketing and sales roles. “Brett’s background working with customers and eye for market ...
Breaking Biology Technology: