Navigation Links
High-throughput analysis of gene regulation, DNA synthesis in Cold Spring Harbor Protocols
Date:2/1/2010

COLD SPRING HARBOR, N.Y. (Mon., Feb. 1, 2010) -- Mapping DNase I hypersensitive sites has long been the standard method for identifying genetic regulatory elements such as promoters, enhancers, silencers, insulators, and locus control regions. Sequences that are nucleosome-depleted, presumably to provide access for transcription factors, are selectively digested by DNase I. Traditional low-throughput methods use Southern blots to then identify these hypersensitive sites. In the February issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc2_10.dtl), Gregory Crawford and colleagues from Duke University (http://www.genome.duke.edu/people/faculty/crawford/) present "DNase-seq: A High-Resolution Technique for Mapping Active Gene Regulatory Elements Across the Genome from Mammalian Cells." DNase-seq is a high-throughput method that identifies DNase I hypersensitive sites across the whole genome by capturing DNase-digested fragments and applying next-generation sequencing techniques. In a single experiment, DNase-seq can identify most active regulatory regions from potentially any cell type, from any species with a sequenced genome. As one of February's featured articles, it is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/2/pdb.prot5384).

The incorporation of thymidine analogues, such as 5-bromo-2'-deoxyuridine (BrdU), into newly synthesized DNA is a powerful tool for analysis of DNA replication, repair and other aspects of DNA metabolism. In "Genome-Wide Analysis of DNA Synthesis by BrdU Immunoprecipitation on Tiling Microarrays (BrdU-IP-chip) in Saccharomyces cerevisiae," Oscar Aparicio and colleagues from the University of Southern California (

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. NIH grants Phylonix Phase II SBIR to develop high-throughput in vivo zebrafish assays
2. New high-throughput screening technique makes probing puzzling proteins possible
3. High-throughput genotyping, protein purification featured in Cold Spring Harbor Protocols
4. Ginkgo SRMs: Tools for product analysis/quality
5. DNA analysis shows true dispersal of protozoa
6. Interacting protein theory awaits test from new neutron analysis tools
7. Analysis of breast and colon cancer genes finds many areas of differences between tumors
8. Tumor genome analysis unveils new insights into lung cancer
9. Powerful integration of lipid metabolic profiling with gene expression analysis
10. Human clones: New U.N. analysis lays out worlds choices
11. More comprehensive analysis of Klamath River basin needed to aid decision makers
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/1/2015)... -- NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the ... mobile commerce market, announces the second shipment of a ... early access pre-order customers. Feedback from ... including Walmart, Target, AT&T, Dunkin, Donuts, Stop & Shop ... all outlets and very easy to use. Several users ...
(Date:3/31/2015)... March 31, 2015   Guidepoint , a leading ... the Post-Surgical Pain Management TRACKER, part of the ... trends in the medical device and therapeutics markets. The ... including treatment volumes, market share, and adoption rates across ... with joint surgery. The Post-Surgical Pain Management ...
(Date:3/24/2015)... 24, 2015 Research and Markets ( ... "Global Iris Recognition Market 2015-2019" report to their ... Recognition market to grow at a CAGR of 23.5 ... covers the present scenario and the growth prospects of ... To calculate the market size, the report considers the ...
Breaking Biology News(10 mins):NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 2NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 3NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 4Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3Global Iris Recognition Market 2015-2019 with 3M Cogent, Bioenable Technologies, Cross Match Technology, Iris ID Systems & Safran Dominating 2
... Olympus America Inc. formally introduced its advanced technology ... invasive single-site surgery through the umbilicus (belly button), ... post-operative pain, less internal/external scarring and improved recovery ... Society of American Gastrointestinal and Endoscopic Surgeons meeting, ...
... the phenomenon called "red tide" is expected to be ... at the Woods Hole Oceanographic Institution (WHOI) and North ... in part on a regional seafloor survey of quantities ... toxin that accumulates in clams, mussels and other shellfish ...
... BOSTON Eating salmon or other fatty fish just once ... to growing evidence that omega-3 fatty acids are of benefit ... Deaconess Medical Center (BIDMC) and reported in today,s on-line issue ... one of the largest studies to investigate the association. ...
Cached Biology News:Olympus advances technology for laparo-endoscopic single-site surgery 2Marine scientists warn of potential for spring, summer red tide outbreak in Gulf of Maine 2Marine scientists warn of potential for spring, summer red tide outbreak in Gulf of Maine 3Eating fatty fish once a week reduces men's risk of heart failure 2Eating fatty fish once a week reduces men's risk of heart failure 3
(Date:5/5/2015)... Franz Inc ., the leading supplier of Semantic ... AllegroGraph, has been named a Champion by Bloor Research in ... AllegroGraph is a high performance Semantic Graph Database that enables ... skyrocketing in popularity and have grown by 400% in the ... by DB-Engines. , “We are excited that Graph and ...
(Date:5/5/2015)... , May 5, 2015  Orexigen Therapeutics, Inc. (Nasdaq: ... first quarter 2015 on Friday, May 8 before the ... live webcast and conference call at 8:30 a.m. Eastern ... call may be accessed by phone by calling (800) ... The webcast can be accessed live on the Investors ...
(Date:5/5/2015)... 2015  Indianapolis-based medical device manufacturer NICO Corporation ... of products including the BrainPath® and Myriad™ technologies ... brain abnormalities and then removing affected tissue. More ... than 50 institutions throughout the United ... included in 12 abstracts, three peer-reviewed publications, and ...
(Date:5/4/2015)... 4, 2015 Tobira Therapeutics, Inc. (NASDAQ: ... development and commercialization of novel treatments for liver ... its merger with Regado Biosciences, Inc. (NASDAQ: RGDO, ... of the merger, Tobira raised $40 million of ... and all current Tobira institutional investors, including the ...
Breaking Biology Technology:Bloor Research Positions AllegroGraph as a ‘Champion’ in Burgeoning Graph Database Market 2Bloor Research Positions AllegroGraph as a ‘Champion’ in Burgeoning Graph Database Market 3NICO Corporation Gains Market Expansion after Multiple Published Clinical Articles Support Access Technology for Deep Brain Lesions 2NICO Corporation Gains Market Expansion after Multiple Published Clinical Articles Support Access Technology for Deep Brain Lesions 3Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 2Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 3Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 4Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 5Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 6
... Pharmaceuticals, Inc. (NYSE Amex: AEN ), a ... for serious illnesses, announced today that it received approval at ... name to Synthetic Biologics, Inc. It is expected that ... name and stock ticker symbol, "SYN", effective as of ...
... of British Columbia professor has developed a new class of ... loss of consciousness in rats, and which are now ... "petit mal seizures," are a symptom of epilepsy, most commonly ... but dazed. The seizures, arising from a flurry of high-frequency ...
... 2012  ERT (NASDAQ: ERT ), a global ... devices to biopharmaceutical sponsors and Contract Research Organizations (CROs), ... to further elevate its market and thought leadership position. ... months, web traffic to ERT,s website increased by 41% ...
Cached Biology Technology:New drugs show promise for preventing 'absence seizures' in children: UBC research 2ERT Launches New Website to Support Significant Commercial Growth 2
12 breakable polypropylene strips of 8 wells each held in a rigid frame. Ideal for antibody assays....
... for in vitro diagnostic assays, all EIA/RIA ... for uniform binding, high optical clarity and ... are part of the Certified Surface Chemistry ... be within QA release specifications. Linkage: This ...
... aqueous solution that contains a purified bovine ... a saline solution, pH 6.5 7.5, ... and 0.02% bromonitrodioxane as a preservative. ... stabilizer for biologically active components in solution, ...
AC input: 230 V...
Biology Products: