Navigation Links
High-throughput analysis of gene regulation, DNA synthesis in Cold Spring Harbor Protocols
Date:2/1/2010

COLD SPRING HARBOR, N.Y. (Mon., Feb. 1, 2010) -- Mapping DNase I hypersensitive sites has long been the standard method for identifying genetic regulatory elements such as promoters, enhancers, silencers, insulators, and locus control regions. Sequences that are nucleosome-depleted, presumably to provide access for transcription factors, are selectively digested by DNase I. Traditional low-throughput methods use Southern blots to then identify these hypersensitive sites. In the February issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc2_10.dtl), Gregory Crawford and colleagues from Duke University (http://www.genome.duke.edu/people/faculty/crawford/) present "DNase-seq: A High-Resolution Technique for Mapping Active Gene Regulatory Elements Across the Genome from Mammalian Cells." DNase-seq is a high-throughput method that identifies DNase I hypersensitive sites across the whole genome by capturing DNase-digested fragments and applying next-generation sequencing techniques. In a single experiment, DNase-seq can identify most active regulatory regions from potentially any cell type, from any species with a sequenced genome. As one of February's featured articles, it is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/2/pdb.prot5384).

The incorporation of thymidine analogues, such as 5-bromo-2'-deoxyuridine (BrdU), into newly synthesized DNA is a powerful tool for analysis of DNA replication, repair and other aspects of DNA metabolism. In "Genome-Wide Analysis of DNA Synthesis by BrdU Immunoprecipitation on Tiling Microarrays (BrdU-IP-chip) in Saccharomyces cerevisiae," Oscar Aparicio and colleagues from the University of Southern California (

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. NIH grants Phylonix Phase II SBIR to develop high-throughput in vivo zebrafish assays
2. New high-throughput screening technique makes probing puzzling proteins possible
3. High-throughput genotyping, protein purification featured in Cold Spring Harbor Protocols
4. Ginkgo SRMs: Tools for product analysis/quality
5. DNA analysis shows true dispersal of protozoa
6. Interacting protein theory awaits test from new neutron analysis tools
7. Analysis of breast and colon cancer genes finds many areas of differences between tumors
8. Tumor genome analysis unveils new insights into lung cancer
9. Powerful integration of lipid metabolic profiling with gene expression analysis
10. Human clones: New U.N. analysis lays out worlds choices
11. More comprehensive analysis of Klamath River basin needed to aid decision makers
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/16/2014)... PALM BEACH GARDENS, Fla. , Sept. 16, ... provider of biometric identity management solutions, announced today ... handheld solution designed to authenticate the identity of ... The Android-based Verifier Sentry rapidly reads ... the credential holder,s ID by matching the biometric ...
(Date:9/15/2014)... processes from digestion to disease resistance. Despite the fact ... on the planet, more is known about belly-button bacteria ... and colleagues working on Panama,s Barro Colorado Island discovered ... home to more than 400 different kinds of bacteria. ... than 7,000 different kinds. , Bacteria in tropical forests ...
(Date:9/15/2014)... has evolved a simple, mechanical solution to avoid ... 11 Science Express. , ... misalignments that lead to infertility, miscarriage, or congenital ... cause these problems, but what exactly goes wrong ... the Wellcome Trust Center for Cell Biology at ...
Breaking Biology News(10 mins):Cross Match Launches New Identity Management Handheld Solution 2Smithsonian scientists discover tropical tree microbiome in Panama 2Cells simply avoid chromosome confusion 2Cells simply avoid chromosome confusion 3
... go through a laboratory version of boot camp may ... of Pittsburgh researchers. In essence, that's the concept behind ... a patient's own souped up dendritic cells, which have ... system in fighting the virus unique to that individual. ...
... affiliate of Harvard Medical School, are the first to ... angiogenesis (new blood vessel growth). The switch, they learned, ... two enzymes (known as PI3K and PLCg) that compete ... fulfill opposite missions, growth and regression, respectively. This finding ...
... at the Harvard Department of Ophthalmology's Schepens Eye Research ... the first to learn why the cornea, the clear ... unique phenomenon that makes vision possible. The key, say ... of the protein VEGFR-3 (vascular endothelial growth factor receptor-3) ...
Cached Biology News:T cells activated to fight HIV basis for dendritic cell therapeutic vaccine 2T cells activated to fight HIV basis for dendritic cell therapeutic vaccine 3Schepens scientists are first to discover angiogenesis switch inside blood vessel cells 2Schepens scientists are first to discover angiogenesis switch inside blood vessel cells 3Scientists discover why cornea is transparent and free of blood vessels, allowing vision 2Scientists discover why cornea is transparent and free of blood vessels, allowing vision 3
(Date:9/16/2014)... 16, 2014 In preparation for VARTECH 2014, ... cybersecurity company focused on authentication and SSO announced today ... ability to generate a OTP through the presentation of ... of user-based RFID authentication, 2FA,s solutions have leveraged the ... many years. RFID authentication is broadly deployed in healthcare ...
(Date:9/16/2014)... 16, 2014 According to Jeff Howell, ... of ITRA Global, new development in Downtown Toronto is ... council approving another 755 storeys of new development last ... cranes will be dominating the Toronto skyline for the ... an unquenchable thirst for new development. , As the ...
(Date:9/15/2014)... solid, liquid, and gasbut much stranger things ... phase transitions occur at the coldest temperatures ... can dramatically transform a material. , ... Brookhaven National Laboratory and Stony Brook University ... zero to isolate and probe these quantum ...
(Date:9/15/2014)... Cancer Immunotherapy Market : ... Therapies is a new market research report announced ... principal types of therapeutics that have become the ... immunotherapy (which is often called "immuno-oncology") in recent ... cellular immunotherapy. , The regular use of ...
Breaking Biology Technology:2FA, Inc. Announces RFID-based One-Time Password (OTP) Authentication 2ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 2ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 3Elusive quantum transformations found near absolute zero 2Elusive quantum transformations found near absolute zero 3Cancer Immunotherapy Market: Immune Checkpoint Inhibitors, Cancer Vaccines, and Adoptive T-cell Therapies 2Cancer Immunotherapy Market: Immune Checkpoint Inhibitors, Cancer Vaccines, and Adoptive T-cell Therapies 3Cancer Immunotherapy Market: Immune Checkpoint Inhibitors, Cancer Vaccines, and Adoptive T-cell Therapies 4Cancer Immunotherapy Market: Immune Checkpoint Inhibitors, Cancer Vaccines, and Adoptive T-cell Therapies 5
... eResearchTechnology, Inc. (ERT), (Nasdaq: ERT ) a ... to bio-pharmaceutical and healthcare organizations and the market leader ... drug development, announced today that its Board of Directors ... and Chief Executive Officer and as a member of ...
... Biopharmaceuticals, Inc. (NASDAQ: KERX ), a biopharmaceutical ... medically important pharmaceutical products for the treatment of cancer ... for the first quarter ended March 31, 2011. ... cash equivalents and investment securities of $23.1 million, as ...
... 2011 Frost & Sullivan Europe New Product Innovation Award ... for its state-of-the-art, high precision Novelda Impulse Radar transceiver ... Radar transceiver is a cutting-edge radar transceiver that incorporates ... unique penetration capabilities," remarks Frost & Sullivan Research Analyst ...
Cached Biology Technology:ERT Announces Appointment of New President and CEO 2ERT Announces Appointment of New President and CEO 3ERT Announces Appointment of New President and CEO 4Keryx Biopharmaceuticals, Inc. Announces First Quarter 2011 Financial Results 2Keryx Biopharmaceuticals, Inc. Announces First Quarter 2011 Financial Results 3Keryx Biopharmaceuticals, Inc. Announces First Quarter 2011 Financial Results 4Frost & Sullivan Award Acknowledges Novelda's Cutting-Edge Product Innovation 2Frost & Sullivan Award Acknowledges Novelda's Cutting-Edge Product Innovation 3Frost & Sullivan Award Acknowledges Novelda's Cutting-Edge Product Innovation 4
Rabbit Anti-Mouse Ym1 Polyclonal Antibody,...
ABgene® supplies a 94kDa ultra-pure recombinant thermostable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli....
... of large numbers of samples may be carried ... which requires minimum operator attention. System control ... and interface CE 4900. The elegant, powerful ... Windows XP and Windows 98 and 2000., The ...
... This system uses the Adept CE 4100 ... Ultra-Fast Scanning UV/Visible detector for added flexibility and ... a trustworthy alternative to a photodiode array detection ... one detector is required for method development and ...
Biology Products: