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High-resolution 3-D imaging draws new picture of Golgi's whereabouts during cell division
Date:12/15/2013

r own, waiting for cytokinesis when the two daughter cells separate and the Golgi body reappears as stacks of membrane-bound cisternae, ready to sort proteins from the reassembled ER.

Powered by their new imaging technologies, which gave them far greater resolution than previously possible, the researchers saw clear support of the ER-linked model -- the enzyme haze sticking close to ER markers with the puncta clustering near ER exits.

For a second line of proof, the NICHD researchers followed up with a pharmacological-based trapping assay that showed Golgi enzymes being held tightly by the ER during mitosis. The results indicate that Golgi enzymes redistribute into the ER during mitosis, and that they must follow an ER export pathway to reform the Golgi at the end of mitosis.

This study not only resolves a basic cellular question but shows what new solutions await as these new technologies give us keener vision and sharper tools.


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Contact: Cathy Yarbrough
cyarbrough@ascb.org
858-243-1814

John Fleischman
jfleischman@ascb.org

American Society for Cell Biology
Source:Eurekalert


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