Navigation Links
High-resolution 3-D imaging draws new picture of Golgi's whereabouts during cell division
Date:12/15/2013

Resolving a fundamental question in cell biology and showing off the powers of new high-resolution 3-D imaging, NIH scientists have discovered where the Golgi apparatus, which sorts newly synthesized proteins for transport inside and outside the cell, goes when it disassembles during cell division, according to research to be presented on Sunday, Dec. 15, at the American Association for Cell Biology (ASCB) annual meeting in New Orleans.

With conventional microscopy techniques, the scientists said they could only watch as the Golgi dissolved into tiny "puncta" and an unresolvable haze. But powerful new imaging techniques allowed the researchers to follow the Golgi through its "choreographed disassembly process," which now appears tightly linked to the endoplasmic reticulum (ER) during cell division, said Dylan Burnette, Ph.D., and Prabuddha Sengupta, Ph.D., and Jennifer Lippincott-Schwartz, Ph.D., of the Eunice Shriver National Institute of Child Health and Human Development (NICHD) in Bethesda, MD.

Cell division by mitosis is the complicated yet critical process by which a mother cell divides into two daughter cells. But first, the mother cell has to pack up her cellular household contents, disassembling and dividing up everything for her soon-to-be-formed daughters.

How cells manage division has been exhaustively studied for over a century and yet basic mysteries remained. Scientists knew that some organelles such as the ER are pulled apart before division but keep their tubular membrane structure intact. Other organelles such as the Golgi, go to pieces after the prophase of mitosis through choreographed disassembly.

But where does the Golgi go once it is in pieces? To answer the question, the NIH researchers started with two plausible theories: In the endoplasmic reticulum (ER)-linked hypothesis, the Golgi puncta and enzyme haze are closely held by the ER; in the non-ER-linked model, the puncta and haze float about on their own, waiting for cytokinesis when the two daughter cells separate and the Golgi body reappears as stacks of membrane-bound cisternae, ready to sort proteins from the reassembled ER.

Powered by their new imaging technologies, which gave them far greater resolution than previously possible, the researchers saw clear support of the ER-linked model -- the enzyme haze sticking close to ER markers with the puncta clustering near ER exits.

For a second line of proof, the NICHD researchers followed up with a pharmacological-based trapping assay that showed Golgi enzymes being held tightly by the ER during mitosis. The results indicate that Golgi enzymes redistribute into the ER during mitosis, and that they must follow an ER export pathway to reform the Golgi at the end of mitosis.

This study not only resolves a basic cellular question but shows what new solutions await as these new technologies give us keener vision and sharper tools.


'/>"/>

Contact: Cathy Yarbrough
cyarbrough@ascb.org
858-243-1814

John Fleischman
jfleischman@ascb.org

American Society for Cell Biology
Source:Eurekalert


Related biology news :

1. A high-resolution endoscope as thin as a human hair
2. Citizen scientists to document biodiversity with high-resolution imagery during summer solstice
3. High-resolution atomic imaging of specimens in liquid by TEM using graphene liquid cell
4. High-tech X-ray imaging technique to offer detailed look at engineered tissue
5. Radiographic imaging exposes relationship between obesity and cancer
6. Visualizing the past: Nondestructive imaging of ancient fossils
7. Kessler Foundation neuroimaging study sheds light on mechanisms of cognitive fatigue in MS
8. Cancer imaging centers get £35 million boost
9. Organic electronics: Imaging defects in solar cells
10. Molecular Imaging Agents: Targets, Technology, Markets, and Commercial Opportunities
11. Texas A&M research contributes to improved ultrasound imaging
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/31/2017)...  Spero Therapeutics, LLC, a biopharmaceutical company founded ... bacterial infections, today announced it has acquired a ... Bono Bio Ltd (PBB) to bolster its pipeline ... of Gram-negative bacteria.   The assets acquired have been ... group company. "The acquisition of these ...
(Date:1/24/2017)... , Jan. 24, 2017 Biopharm Reports ... the laboratory use of nuclear magnetic resonance spectroscopy ... end-users and profiled current practices, developments, trends and ... well as growth and opportunities. These areas include ... NMR instruments, needs and innovation requirements, hyphenated NMR ...
(Date:1/19/2017)... , Jan. 19, 2017 ... user experience and security for consumer electronics, and ... payment processing systems and cybersecurity solutions, today announced ... enterprises and financial institutions worldwide to bolster security ... the end-to-end secure user authentication platforms they offer, ...
Breaking Biology News(10 mins):
(Date:2/22/2017)... ... 2017 , ... ProMIS Neurosciences (“ProMIS” or the “Company”), a ... announced it has issued a scientific white paper entitled “Results from recent Alzheimer’s ... commentaries from ProMIS’s scientific team offering insight into the Company’s product portfolio and ...
(Date:2/22/2017)... Feb. 22, 2017 Aethlon Medical, Inc. (Nasdaq: ... a study that validated the ability of the Aethlon ... with increased mortality in immune-suppressed sepsis patients and also ... The objective of the study was to validate ... Epstein-Barr virus (EBV) and Herpes Simplex virus 1 (HSV1) ...
(Date:2/21/2017)... Synthetic Biologics, Inc. (NYSE MKT: SYN), a ... protect and restore the health of patients, intends to report operational ... on Thursday, March 2, 2017, and to host a conference call ... the call is as follows: U.S. (toll free): 1-888-347-5280 ... ...
(Date:2/21/2017)... , ... February 21, 2017 , ... ... that Dr. Trevor Heritage has joined its executive team to lead the development ... to provide insights to help improve the diagnosis and treatment of cancer. The ...
Breaking Biology Technology: