Navigation Links
Hershey scientists improve methods for analysis of healthful cocoa compounds
Date:2/14/2011

Two scientific publications report on improved methods for determining the amounts of flavanol antioxidants in cocoa and chocolate. The research, sponsored by The Hershey Center for Health and Nutrition, was a collaboration between scientists at The Hershey Company and other scientific laboratories.

Scientists at Planta Analytica (Danbury, CT) isolated and separated cocoa flavanol antioxidants on a large scale. The Hershey scientists and collaborating scientists at the Pennsylvania State University-M.S. Hershey Medical Center (Hershey, PA) teamed up to determine the purity of these flavanols by HPLC and by Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) mass spectroscopy. The isolated compounds were then used as standards in the determination of flavanol cocoa antioxidants in a cocoa powder and a dark chocolate.

"We believe this represents the first large scale purification of standards for flavanol antioxidant determination" said Dr. Jeffrey Hurst of the Hershey Center for Health and Nutrition. "Prior to this, only dimers were commercially available. With a full series of standards, our flavanol determinations are not only more accurate, but the values are much higher, between 40% to 100% higher, than previously published methods using proprietary standards." This also means that standards are commercially available to various laboratories. This collaborative work was published in the online journal

In other research published in the Journal Association of Official Analytical Chemists, scientists from The Hershey Company and Brunswick Laboratories (Newton, MA) reported on the development of a new method for determining total procyanidins. This method is a colorimetric test based on the specific reaction of dimethylaminocinnamaldehyde (DMAC) with flavanols. The method measures flavanol monomers, including EGCG, as well as higher flavanol polymers. The method which is standardized using a commercially available flavanol dimer, was validated at two Brunswick Laboratories facilities and at Hershey with all three laboratories providing comparable results at the 95% confidence level. "The specific reaction of DMAC with the flavanols has been known since the 1950s. This method is a simple and quick way to measure total procyanidins in cocoa and chocolate" said Dr. Mark Payne of the Hershey Center for Health and Nutrition. "Compared to the HPLC method, which separates individual compounds, this method gives one number, which importantly includes polymers of flavanols beyond ten."

"These reports are part of an ongoing series of publications, by Hershey and its collaborators, designed to improve upon the methods to determine flavanol antioxidants from cocoa and chocolate," said Dr. David Stuart, of the Hershey Center for Health and Nutrition. "We want to make these methods generally available to the chocolate industry initially, with the intent of having uniformly agreed upon methods of determining the level of these important molecules."

These new methods can be used in research and other applications involving dietary intake of cocoa and chocolate, clinical interventions and food standardization".


'/>"/>

Contact: Debra Miller
debramiller@hersheys.com
717-534-5686
The Hershey Company
Source:Eurekalert

Related biology news :

1. Jefferson scientists deliver toxic genes to effectively kill pancreatic cancer cells
2. Scientists identify novel inhibitor of human microRNA
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. MU scientists go green with gold, distribute environmentally friendly nanoparticles
5. Scientists identify gene that may contribute to improved rice yield
6. Scientists discover why a mothers high-fat diet contributes to obesity in her children
7. MU scientists see how HIV matures into an infection
8. Earth scientists keep an eye on Texas
9. Thinking it through: Scientists call for policy to guide biofuels industry toward sustainability
10. Scientists identify a molecule that coordinates the movement of cells
11. Scientists Find new migratory patterns for Mediterranean and Western Atlantic bluefin tuna
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/13/2017)... Germany , March 13, 2017 Future of security: ... ... DERMALOGs Face Matching enables to match face ... forms the basis to identify individuals. (PRNewsFoto/Dermalog Identification Systems) ... DERMALOG,s "Face Matching" is the fastest software for biometric ...
(Date:3/9/2017)... MOUNTAIN VIEW, Calif. , March 9, 2017 /PRNewswire/ ... 23andMe , the leading personal genetics company, are ... can now provide customers with personalized nutrition plans that ... biometrics, but also genetic markers impacting how their body ... personalized food decision support platform uses biometrics such as ...
(Date:3/7/2017)... SALT LAKE CITY , March 7, 2017 /PRNewswire/ ... solutions that help top global companies identify the best ... Leonard as Chief Sales Officer (CSO) and ... and Kucer,s appointments round out a seasoned executive team poised ... year and beyond, building on a year of record ...
Breaking Biology News(10 mins):
(Date:4/20/2017)... ... April 20, 2017 , ... Open Therapeutics and ... (IP) sharing and commercialization model. , The Center for Advancing Innovation helps institutions ... this effort is bringing the IP to the attention of the entrepreneurial community ...
(Date:4/20/2017)... For today, Stock-Callers.com redirects investors, attention to Biotechnology, ... research aimed at treating diseases and medical conditions. Under evaluation ... (NASDAQ: KERX), Kite Pharma Inc. (NASDAQ: KITE), and ZIOPHARM Oncology ... complimentary research reports on these stocks now at: ... http://stock-callers.com/registration Biostage ...
(Date:4/19/2017)... ... 18, 2017 , ... Alisa Wright, founder and CEO of Singota Solutions ... of Pharmacy in Lafayette, Indiana. , The Distinguished Alumni Award was established in ... and other scientific endeavors. , Wright began her career in the pharmaceutical industry ...
(Date:4/19/2017)... ... April 19, 2017 , ... ThermaGenix, the PCR Improvement ... adds to several other early achievements at ThermaGenix, including the business formation and ... , ThermaGenix will use proceeds from the Series A-1 round to:, ...
Breaking Biology Technology: