Navigation Links
Hershey scientists improve methods for analysis of healthful cocoa compounds
Date:2/14/2011

Two scientific publications report on improved methods for determining the amounts of flavanol antioxidants in cocoa and chocolate. The research, sponsored by The Hershey Center for Health and Nutrition, was a collaboration between scientists at The Hershey Company and other scientific laboratories.

Scientists at Planta Analytica (Danbury, CT) isolated and separated cocoa flavanol antioxidants on a large scale. The Hershey scientists and collaborating scientists at the Pennsylvania State University-M.S. Hershey Medical Center (Hershey, PA) teamed up to determine the purity of these flavanols by HPLC and by Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) mass spectroscopy. The isolated compounds were then used as standards in the determination of flavanol cocoa antioxidants in a cocoa powder and a dark chocolate.

"We believe this represents the first large scale purification of standards for flavanol antioxidant determination" said Dr. Jeffrey Hurst of the Hershey Center for Health and Nutrition. "Prior to this, only dimers were commercially available. With a full series of standards, our flavanol determinations are not only more accurate, but the values are much higher, between 40% to 100% higher, than previously published methods using proprietary standards." This also means that standards are commercially available to various laboratories. This collaborative work was published in the online journal

In other research published in the Journal Association of Official Analytical Chemists, scientists from The Hershey Company and Brunswick Laboratories (Newton, MA) reported on the development of a new method for determining total procyanidins. This method is a colorimetric test based on the specific reaction of dimethylaminocinnamaldehyde (DMAC) with flavanols. The method measures flavanol monomers, including EGCG, as well as higher flavanol polymers. The method which is standardized using a commercially available flavanol dimer, was validated at two Brunswick Laboratories facilities and at Hershey with all three laboratories providing comparable results at the 95% confidence level. "The specific reaction of DMAC with the flavanols has been known since the 1950s. This method is a simple and quick way to measure total procyanidins in cocoa and chocolate" said Dr. Mark Payne of the Hershey Center for Health and Nutrition. "Compared to the HPLC method, which separates individual compounds, this method gives one number, which importantly includes polymers of flavanols beyond ten."

"These reports are part of an ongoing series of publications, by Hershey and its collaborators, designed to improve upon the methods to determine flavanol antioxidants from cocoa and chocolate," said Dr. David Stuart, of the Hershey Center for Health and Nutrition. "We want to make these methods generally available to the chocolate industry initially, with the intent of having uniformly agreed upon methods of determining the level of these important molecules."

These new methods can be used in research and other applications involving dietary intake of cocoa and chocolate, clinical interventions and food standardization".


'/>"/>

Contact: Debra Miller
debramiller@hersheys.com
717-534-5686
The Hershey Company
Source:Eurekalert

Related biology news :

1. Jefferson scientists deliver toxic genes to effectively kill pancreatic cancer cells
2. Scientists identify novel inhibitor of human microRNA
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. MU scientists go green with gold, distribute environmentally friendly nanoparticles
5. Scientists identify gene that may contribute to improved rice yield
6. Scientists discover why a mothers high-fat diet contributes to obesity in her children
7. MU scientists see how HIV matures into an infection
8. Earth scientists keep an eye on Texas
9. Thinking it through: Scientists call for policy to guide biofuels industry toward sustainability
10. Scientists identify a molecule that coordinates the movement of cells
11. Scientists Find new migratory patterns for Mediterranean and Western Atlantic bluefin tuna
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/28/2017)... The report "Video Surveillance Market ... Storage Devices), Software (Video Analytics, VMS), and Service (VSaaS, ... to 2022", published by MarketsandMarkets, the market was valued ... to reach USD 75.64 Billion by 2022, at a ... year considered for the study is 2016 and the ...
(Date:3/24/2017)... Research and Markets has announced the addition of the ... Forecast to 2025" report to their offering. ... The Global Biometric Vehicle Access System ... over the next decade to reach approximately $1,580 million by 2025. ... forecasts for all the given segments on global as well as ...
(Date:3/22/2017)... March 21, 2017   Neurotechnology , a ... technologies, today announced the release of the ... provides improved facial recognition using up to 10 ... single computer. The new version uses deep neural-network-based ... and it utilizes a Graphing Processing Unit (GPU) ...
Breaking Biology News(10 mins):
(Date:7/18/2017)... ... July 18, 2017 , ... G-CON today announced that ... Office for its Patent Applications 14/858,857 and 13/669,785 both entitled Modular, Self-Contained, Mobile ... further expand the protection of G-CON’s R&D investments and validate the G-CON platform ...
(Date:7/18/2017)... ... July 18, 2017 , ... Allotrope Foundation ... the first phase of the Allotrope Framework for commercial use. , The Bio-IT ... to “not only elevate the critical role of information technology in modern biomedical ...
(Date:7/17/2017)... ... , ... Whitehouse Laboratories is excited to announce that it ... of ISO 80369 standard test procedures. The ISO 80369 series of eight test ... With this recent expansion, Whitehouse Labs becomes one of the only facilities in ...
(Date:7/17/2017)... ... July 17, 2017 , ... ... announced safety software company AB Cube has joined its eHealth App ... advance technology innovation across life sciences and healthcare. Under the partnership, AB ...
Breaking Biology Technology: