Navigation Links
H1N1 influenza adopted novel strategy to move from birds to humans
Date:12/8/2009

the polymerase overcomes that inhibition and allows the virus to replicate. Apparently, Mehle said, when the amino acid glutamic acid typical of most bird virus polymerases is changed to a lysine, typical of human polymerases, the surface charge of the subunit changes from acidic (negatively charged) to basic (positively charged) and allows assembly of the subunits. Previous studies in mammals have shown that a lysine in that position enhances polymerase activity, increases viral replication and transmission, and in some cases, is associated with increased pathogenicity and death.

In their new study, Mehle and Doudna found that H1N1 has two rare mutations in the second subunit: a serine at position 590 and an arginine at position 591. This combination, which is most common in pigs, apparently has the same effect on surface charge as the mutation at position 627, allowing the polymerase complex to form and function in human cells.

Mehle noted that, in addition to such point mutations, flu viruses also mix and match the three subunits. Both the 1957 and 1968 viruses had polymerases composed of a first subunit from a bird and the other two subunits from humans. H1N1 has a human-like first subunit, while the second and third are bird-like hence the need for a mutation in the second subunit to make it more human-like.

To see which other combinations might make H1N1 more virulent, they mixed human, avian and pig subunits in culture, replicating the pig "mixing vessel," Mehle said. Several combinations with a human third subunit increased the activity of the polymerase enzyme when other mutations were not present in the second subunit. Viruses with this alteration are now being tested in human cell culture to see if they are more virulent.

"In addition to having individual amino acid changes affecting the ability of the virus to transmit across species and be more pathogenic, we need to think about these entire gene segments being exc
'/>"/>

Contact: Robert Sanders
rsanders@berkeley.edu
510-643-6998
University of California - Berkeley
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. St. Jude influenza survey uncovers key differences between bird flu and human flu
2. New field-deployable biosensor detects avian influenza virus in minutes instead of days
3. Influenza vaccine causes weaker immune response for children of rural Gabon than in semi-urban areas
4. Tamiflu effective for treatment and prevention of influenza in children 1 year and older
5. Getting wise to the influenza virus tricks
6. Tracking influenzas every movement
7. Mobilizing white blood cells to the lung: New discovery could lead to an improved influenza vaccine
8. Genetic evidence for avian influenza movement from Asia to North America via wild birds
9. K-State researcher finds 1918 flu resulted in current lineage of H1N1 swine influenza viruses
10. SRI International to screen drugs that fight 2009 H1N1 influenza A
11. The challenges of avian influenza virus: Mechanism, epidemiology and control
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/10/2014)... 2014 You,ve been here before: you desperately need ... your password, site key or the answer to your secret ... teacher? Today, Hoyos Labs , a ... will finally put an end to the frustration that comes ... . 1U leverages a user,s smartphone to acquire his or ...
(Date:12/10/2014)... N.C. , Dec. 9, 2014  Wake Forest Baptist ... education building for its School of Medicine. Funding for ... larger capital campaign that will be publicly launched next ... be located in the former 60 series R.J. Reynolds ... Innovation Quarter. Construction will begin immediately with plans to ...
(Date:12/10/2014)... , Dec. 08, 2014 Research and ... the addition of the "Biometrics Market in ... http://photos.prnewswire.com/prnh/20130307/600769 ... projects such as rural banking and upgradation of ... trends witnessed in the market. Besides the aforementioned ...
Breaking Biology News(10 mins):The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2
... This release is available in German . ... and know-how in micro- and nanotechnologies for users from ... by KIT is starting operation: Ten leading institutions from ... available a total of 36 high-tech installations along with ...
... most invasive plants introduced to the U.S. from Europe and ... the natives. A new study indicates that eventually, however, its ... becomes less potent. The study, in Proceedings ... the first to show that evolutionary forces can alter the ...
... Institute at Oregon State University suggests that some natural food ... prevent cancer, may be able to play a more significant ... drugs that are now used in chemotherapy. A new ... examined the activity of chlorophyllin and found that, on ...
Cached Biology News:Scientific pathways into the nanoworld 2Scientific pathways into the nanoworld 3Over time, an invasive plant loses its toxic edge 2Over time, an invasive plant loses its toxic edge 3Natural compounds, chemotherapeutic drugs may become partners in cancer therapy 2Natural compounds, chemotherapeutic drugs may become partners in cancer therapy 3
(Date:12/24/2014)... 2014 Earlier this year in a ... director of the Adult Stem Cell Technology Center, LLC ... and under appreciated unique property of adult tissue stem ... Cells: Misunderstood in the Past, Important for the Future,” ... He gave the address at the 4th World ...
(Date:12/24/2014)... 23, 2014 The report ... as its definition, classification, application and industry ... manufacturing process, and product cost structure. Production ... The analysis also covers upstream raw materials, ... development trend and proposals. In the end, ...
(Date:12/24/2014)... , Dec. 23, 2014 China ... the "Company"), a leading fully integrated plasma-based biopharmaceutical ... announced that its majority-owned subsidiary, Shandong Taibang Biological ... ("GMP") certification from the China Food and Drug ... production facility. As previously disclosed in the Company,s ...
(Date:12/24/2014)... 2014  Rock Creek Pharmaceuticals, Inc., (NASDAQ: ... Clinical Trial Application (CTA) with the United ... seeking regulatory approval to initiate clinical trials for the ... Contingent on the Company receiving CTA regulatory approval, ... to assess the safety, tolerability and dose escalation of ...
Breaking Biology Technology:Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4Worldwide Methyl Mercaptan Market 2015-2020 Forecasts on Development & Trends Now Available at ReportsnReports.com 2China Biologic Receives GMP Certification for New Coagulation Factor Facility 2China Biologic Receives GMP Certification for New Coagulation Factor Facility 3Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 2Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 3
... Gene arrays are solid supports upon which a , ... have been placed at , ... array analysis, a nucleic acid-containing sample is labeled , ... on the array. (In the literature, the term ...
... Ambion has developed a system for , ... on in vitro , transcription, ... (patent pending). This kit produces transfection-ready siRNA , ... researchers at the Salk Institute describe the successful , ...
... The Silencer siRNA , ... column purification to prepare transfection-ready siRNAs. With , ... is one example of how siRNAs prepared by the , ... in gene silencing studies. , ...
Cached Biology Technology:Array Analysis: The Basics 2Array Analysis: The Basics 3Array Analysis: The Basics 4Array Analysis: The Basics 5Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs 2Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs 2