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Gold nanoantennas detect proteins
Date:3/14/2012

Scientists at Johannes Gutenberg University Mainz (JGU) in Germany have developed a new method of observing individual proteins. Detailed knowledge of the dynamics of proteins is necessary in order to understand the related biological processes that occur on the molecular level. To date, this information has been obtained by means of labeling proteins with fluorescent substances, but unfortunately this changes the proteins under investigation and thus influences the biological processes that are to be observed. "Our method allows live tracking of individual proteins without having to label them first," explains Professor Dr. Carsten Snnichsen of the Institute of Physical Chemistry at JGU. "We are now gaining entirely new insights into molecular processes and can see, for example, how things are constantly in motion even on the very smallest scale."

The method developed by the group of Mainz chemists led by Carsten Snnichsen is based on the use of gold nanoparticles. These serve as glistening nanoantennas that, when they detect individual unlabeled proteins, slightly change their frequency or, in other words, their color. These tiny color changes can be observed using the technique developed in Mainz. "This is an enormous leap forward technologically: We have managed to achieve a very high time resolution for the observation of individual molecules," says Snnichsen. It is thus now possible to precisely observe the dynamics of a protein molecule down to the millisecond.

The opportunity to detect individual protein molecules also opens up completely new horizons. It has thus become practicable to track the fluctuation of protein population densities and observe protein adsorption processes in real time, among other things. "We can see how molecules move, how they dock at particular locations, and how they fold this has given us a window into the molecular world," explains Dr. Irene Ament, a member of Snnichsen's group. This new technology may
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Contact: Carsten Soennichsen
carsten.soennichsen@uni-mainz.de
49-613-139-24313
Johannes Gutenberg Universitaet Mainz
Source:Eurekalert

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