Navigation Links
Going with the flow
Date:8/28/2011

tin, one of the most abundant protein in animal cells, forms dynamic filament networks that play a crucial role in many cellular processes, including cell migration, intracellular transport.

To find out whether and how actin might play a role in the position of the meiotic spindle, postdoctoral researcher and first author Kexi Yi, Ph.D., incubated mouse oocytes with several known inhibitors of the actin cytoskeleton. "Within minutes of applying CK-666, a brand new and very specific inhibitor, the spindle drifted away from the cortical cap towards the center of the oocyte."

CK-666 inhibits the Arp2/3 complex, a major regulator of the actin cytoskeleton that is known to play a role in cell locomotion and membrane trafficking. It binds to existing actin filaments and initiates the growth of new "branch" filaments. Further experiments revealed that myosin-II contractility, better known for producing muscle contractions, pushes the spindle away from the cortical cap when the Arp2/3 complex is inhibited.

Previous work by Li and her team had shown that meiotic chromosomes, when positioned close to the cortex of an oocyte in meiosis II, induces the formation of a cortical actin cap by propagating the regulatory signal from the Ran protein. When Yi tested the effect of intercepting the Ran signal, they found that Ran also regulates Arp2/3 localization and by extension, spindle position.

Yi then turned to high-resolution time-lapse confocal microscopy and spatiotemporal correlation spectroscopy (STICS), in collaboration with Stowers imaging experts, Jay Unruh, Ph.D., and Brian Slaughter, Ph.D., both co-authors on the paper, to track the dynamics of the cytoplasmic actin network in oocytes labeled with a live F-actin probe.

"STICS analysis showed that the actin flow originates at the cortical cap and continues down along both sides of the lateral cortex before it converges near the center of the oocyte and reverses direction to
'/>"/>

Contact: Gina Kirchweger
gxk@stowers.org
816-806-1036
Stowers Institute for Medical Research
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. How mirror neurons allow us to learn and socialize by going through the motions in the head
2. New infant feeding and obesity research adds insight to ongoing issue
3. Lobster traps going high tech
4. Going bananas for sustainable research -- scientists create fuel from African crop waste
5. A heart healthy diet and ongoing, moderate physical activity may protect against cognitive decline
6. WPI receives $1.3 million in federal awards for ongoing research in the life sciences
7. Going green on hold: Man-made activities can affect blue haze, worlds weather
8. Ongoing human evolution could explain recent rise in certain disorders
9. Keystone Symposia awarded $1.37 million, 5-year NIH grant to fund ongoing diversity efforts
10. Going out on a (redwood tree) limb
11. Going green: New program provides vital support for plant scientists
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Going with the flow
(Date:9/30/2014)... have in common? Unlike most eukaryotic multicellular organisms ... these organisms are all polyploid, meaning they have three ... 3 and 4 sets of chromosomes, respectively, and strawberries ... fact most plant species are polyploid. Polyploidy, or genome ... only recently, with the development of molecular tools, has ...
(Date:9/30/2014)... Ecker, a Salk professor and Howard Hughes Medical ... have been named recipients in the 2014 round ... (NIH) through the BRAIN (Brain Research through Advancing ... The grant, announced September 30, provides more than ... over three years. , The BRAIN Initiative, launched ...
(Date:9/30/2014)... of Medicine reports positive results of a phase ... subset of lung cancer marked by rearrangement of the ... with advanced non-small cell lung cancer testing positive for ... with 3 complete responses and 33 partial responses. Median ... disease to resume its growth after being slowed by ...
Breaking Biology News(10 mins):Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 3Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 4Salk scientists receive $3 million for BRAIN Initiative grant 2NEJM: Crizotinib effective in Phase 1 trial against ROS1 lung cancer 2
... pollution is costing each person in Europe around 130 - ... Nitrogen Assessment (ENA) is launched at a conference today in ... from 21 countries and 89 organizations, estimates that the annual ... - 280 billion (70 -320 billion), more than double the ...
... COLUMBUS, Ohio Stress not only sends the human immune ... the trillions of bacteria that work and thrive inside our ... important because those bacteria play a significant role in triggering ... prepared to quickly spring into action in the face of ...
... DETROIT For nearly two decades following the 1991 Gulf War, ... conflict: an unexplainable cluster of symptoms including but not limited ... 2008 that a scientific panel from the U.S. Department of ... served in the Gulf War were suffering from combinations of ...
Cached Biology News:Excessive nitrogen harms the economy and environment -- first Europe-wide assessment published 2Stress wrecks intestinal bacteria, could keep immune system on idle 2WSU receives DOD grant to determine common genetic link among Gulf War Illness patients 2
(Date:10/1/2014)... (PRWEB) October 01, 2014 As the world ... no room for doubt that this century would mark the ... the field of medicine science. The fact that the world ... not unknown to the scientific community. Almost half of these ... therefore can be prevented with medical care and intervention. While ...
(Date:10/1/2014)... Jolla, Calif. (PRWEB) September 30, 2014 ... announced the initial round of grants for the ... an undertaking that aims to develop and revolutionize ... circuit functions of the human brain. This first ... for fiscal year 2014 was allocated to more ...
(Date:10/1/2014)... New applications of structures and materials ... in nature over millennia are featured in a ... applications in biophotonics in the October issue of the ... published by SPIE, the international society for optics and ... peer-reviewed articles are accessible via open access. , “Biomimetic ...
(Date:10/1/2014)... Centreville, VA (PRWEB) October 01, 2014 ... the Diagnostic, Clinical Therapeutics, Healthcare IT, and Laboratory Testing ... and regulatory positions at the director level and above ... payers struggling to stay on top of rapid breakthroughs ... a result, many diagnostic companies have been forced to ...
Breaking Biology Technology:Fighting Mortality and Morbidity of Chronic Health Disorders during Open Access Week 2Fighting Mortality and Morbidity of Chronic Health Disorders during Open Access Week 3NIH Awards 15 Grants to CHI Members for BRAIN Initiative Research 2NIH Awards 15 Grants to CHI Members for BRAIN Initiative Research 3Biomimetic and bioinspired materials enable new healthcare options, reports Journal of Biomedical Optics 2Biomimetic and bioinspired materials enable new healthcare options, reports Journal of Biomedical Optics 3Slone Partners Meets Growing Demand as Industry Focuses on Reimbursement & Regulation 2
... 1, 2011 Elsevier, a world-leading provider ... today announced the availability of Elsevier Urban & ... the life and health sciences, on SciVerse ScienceDirect ... under the renowned German imprint Urban & Fischer, contain ...
... researchers at the University of Toronto have developed the ... This result enables a flexible form factor, not to ... which currently relies on rigid glass. The ... Nature Photonics . OLEDs provide high-contrast and ...
... Moore,s Law, hardly a law but undeniably a persistent trend, ... transistors that fit on a chip roughly doubles. It,s why ... players to movie cameras, from tablets to supercomputers grow ... expensive. Whether the trend can continue until it runs up ...
Cached Biology Technology:Elsevier Announces Availability of Urban & Fischer Journal Archives on SciVerse ScienceDirect 2University of Toronto researchers create world's most efficient flexible OLED on plastic 2A SHARP new microscope for the next generation of biochips 2A SHARP new microscope for the next generation of biochips 3A SHARP new microscope for the next generation of biochips 4