Navigation Links
Going live with click chemistry
Date:10/30/2007

ent, a substituted cyclooctyne, possesses ring strain and electron-withdrawing fluorine substituents that together promote the cycloaddition with azides installed metabolically into biomolecules, said Bertozzi.

This copper-free click reaction of azides and DIFO combines the biocompatibility of the Staudinger ligation [a highly successful labeling reaction previously developed in Bertozzis lab] with the fast reaction kinetics of click chemistry.

Bertozzi says their copper-free click chemistry technique can be used to probe any biomolecule that can be labeled with an azide, including glycans, proteins and lipids. She and her group are now using this technique to study glycan trafficking how glycans and their associated scaffolds move around inside cells and on cell surfaces. They have made their DIFO reagents available to other research groups and are in discussions with potential commercial suppliers to make the reagents widely available to the bio research community.

Direct imaging of glycan trafficking under conditions of cell stimulation or pharmacological intervention can be performed in cells, tissues, or even whole organisms, said Bertozzi. More broadly, other metabolites, post-translational modifications, enzyme activities, and site-specific labeled proteins can be monitored in real time and in living systems with our copper-free click chemistry.


'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory  
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Going To Extremes To Improve Human Health
2. Mayan stingless bee keeping: Going, going, gone?
3. The shape of things to come: Morphology database going global
4. Watch your step when the going gets rough
5. When the going gets tough, slime molds start synthesizing
6. EGF receptor activation prevents microbes from going more than skin deep
7. Birds going extinct faster due to human activities
8. Ongoing collapse of coral reef shark populations
9. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
10. Breakthrough in micro-device fabrication combines biology and synthetic chemistry
11. Male elephants woo females with precise chemistry
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Going live with click chemistry
(Date:7/28/2014)... suggests that the effects of a commonly used anesthetic ... , The study, published in this week,s issue of ... biologists at the University of California, San Diego and ... to concerns, arising from multiple studies on humans over ... may increase their susceptibility to long-term cognitive and behavioral ...
(Date:7/28/2014)... identified areas of the Earth that are high priorities ... Europe is particularly vulnerable, as it has the lowest ... of any continent in ,refugia, areas of biological ... remain relatively constant during times of great environmental change. ... in Scandinavia and Scotland. , The biggest refugia ...
(Date:7/28/2014)... that can help advance the genetic engineering of ... has been developed by researchers with the U.S. ... a multi-institutional partnership led by Lawrence Berkeley National ... an assay that enables scientists to identify and ... components in the biosynthesis of plant cell walls. ...
Breaking Biology News(10 mins):Study suggests disruptive effects of anesthesia on brain cell connections are temporary 2Study suggests disruptive effects of anesthesia on brain cell connections are temporary 3Study finds Europe's habitat and wildlife is vulnerable to climate change 2How sweet it is 2How sweet it is 3
... aspires to a lifetime of addiction. But their brains ... journal Neuropsychopharmacology this week, Rockefeller University researchers ... can sustain lifelong and permanent changes in their reward ... and make such adolescents more vulnerable to the drug,s ...
... of Palm Vein Authentication Technology with, Single-Sign On Solutions ... ... Passwords, SUNNYVALE, Calif., Sept. 9 Fujitsu Computer ... products including,hard disk drives, peripherals and biometric security solutions, ...
... novel way to improve survival and recovery rate after ... Stem Cell Research by scientists at Singapore,s Institute ... and The Netherlands, University Medical Center Utrecht. ... the first non-cell based therapeutic application of human embryonic ...
Cached Biology News:Abuse of painkillers can predispose adolescents to lifelong addiction 2Fujitsu Announces PalmSecure(TM) LOGONDIRECTOR 2Fujitsu Announces PalmSecure(TM) LOGONDIRECTOR 3Fujitsu Announces PalmSecure(TM) LOGONDIRECTOR 4Human embryonic stem cell secretions minimized tissue injury after heart attack 2Human embryonic stem cell secretions minimized tissue injury after heart attack 3
(Date:7/28/2014)... It’s a hard truth: Nearly half of recent veterans who ... are failing to obtain a diploma or certificate within a ... an analysis by The Resume Place , a major ... college majors, which leads to underemployment, unemployment or just plain ... of post-9/11 veterans using GI Bill education benefits ...
(Date:7/28/2014)... DUBLIN , July 28, 2014 Research ... "Global Particle Size Analysis Market 2014-2018" report to their ... Particle size analysis is a technique used to determine ... powder or liquid. It is crucial to analyze the size ... material. Therefore, particle size analysis is performed to determine the ...
(Date:7/25/2014)... , July 25, 2014 Amgen (NASDAQ: ... Directors today declared a $0.61 per share dividend for ... paid on Sept. 5, 2014, to all stockholders of ... 14, 2014.  About Amgen Amgen is ... suffering from serious illnesses by discovering, developing, manufacturing and ...
(Date:7/25/2014)... July 25, 2014 According to a ... Drugs Market - Global Industry Analysis, Size, Share, Growth, ... drugs market is estimated at USD 161,056.5 million in ... of 10.1% from 2014 to 2020, to reach an ... The biological drugs market is witnessing a significant growth ...
Breaking Biology Technology:Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 2Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 3Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 4Global Particle Size Analysis Market 2014-2018 2Amgen Announces 2014 Third Quarter Dividend 2Amgen Announces 2014 Third Quarter Dividend 3Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3
... Registry reveals that 70% of deaths in the stable stroke ... other than stroke, NICE, France, May 15 New ... Congress, highlight that among,those who die after a stroke the ... overall death rate is 4.45%, with,3.23% of these CV deaths ...
... , Chinese researchers have recently made a golden ... is a large ring-shaped molecule containing 36 gold atoms. The ... Universities of Beijing, Hong Kong, and Nanjing report their unusual ... is held together exclusively by goldgold bonds and is thus ...
... opportunities for micro and nano technologies. These challenges are ... the control and understanding of their behaviour. , This ... fibre analysis at the micro and nano-scale using state-of-the ... techniques. , Topics include fundamental effects of topography in ...
Cached Biology Technology:Stable Stroke Patients More Likely to Die From a Nonstroke Cardiovascular Event Than a Further Stroke 2Stable Stroke Patients More Likely to Die From a Nonstroke Cardiovascular Event Than a Further Stroke 3Stable Stroke Patients More Likely to Die From a Nonstroke Cardiovascular Event Than a Further Stroke 4Stable Stroke Patients More Likely to Die From a Nonstroke Cardiovascular Event Than a Further Stroke 5Golden nanocrown 2Micro and Nano Scale Characterization of Fibers 2
EML1 (K-16)...
Bovine Renal Artery Endothelial Cells (BRAEC) (>500,000 cells)...
Bovine Brain Microvascular Endothelial Cells (BBMVEC) (>500,000 cells)...
... ,• Eliminates nonspecific products due to ... specificity allows extension through DNA regions which ... Leaves an 'A' overhang ,TEMPase II ... DNA Polymerase that remains inactive at ...
Biology Products: