Navigation Links
Going live with click chemistry
Date:10/30/2007

copper-catalyzed azide-alkyne reaction that makes it possible for certain chemical building blocks to click together in an irreversible linkage. Since its introduction in 2001 by the Nobel laureate chemist Karl Barry Sharpless of the Scripps Research Institute, the copper-catalyzed azide-alkyne reaction has proven extremely valuable for attaching small molecular probes to various biomolecules in a test tube or on fixed cells. However, its use for biomolecule labeling in live cells or organisms is prohibited by the requirement of a cyotoxic copper catalyst.

For the past several years, Bertozzi has been developing new techniques for studying glycans. Even though glycans are ubiquitous on the surfaces of most cells and play a critical role in intercellular communications, methods for studying them have lagged behind other biomolecules.

Glycans mediate a variety of cell surface recognition events such as bacterial and viral binding to host cells and leukocyte adhesion during an inflammatory response, said Bertozzi. In addition to their cell surface roles, glycans can regulate many intracellular processes, including trafficking of proteins to the lysosome and transcription and translation.

There is great scientific interest in monitoring the dynamics of glycans as they move about within cells and on the cell surface, but the means to tag glycans with imaging probes has been lacking, thereby prohibiting such studies. Bertozzi and her coworkers had previously shown that glycans can be metabolically labeled with azides, permitting their chemical tagging with imaging probes through click chemistry, but the cytotoxicity of the click reaction would not allow dynamic imaging of live cells.

To apply click chemistry to glycans, Bertozzi and her colleagues designed a new reagent called difluorinated cyclooctyne or DIFO, that reacts with azides rapidly at physiological temperatures without the need for a toxic catalyst.

Our critical reag
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory  
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Going To Extremes To Improve Human Health
2. Mayan stingless bee keeping: Going, going, gone?
3. The shape of things to come: Morphology database going global
4. Watch your step when the going gets rough
5. When the going gets tough, slime molds start synthesizing
6. EGF receptor activation prevents microbes from going more than skin deep
7. Birds going extinct faster due to human activities
8. Ongoing collapse of coral reef shark populations
9. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
10. Breakthrough in micro-device fabrication combines biology and synthetic chemistry
11. Male elephants woo females with precise chemistry
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Going live with click chemistry
(Date:7/11/2014)... Conservation Society shows that no-take zones in Belize ... as lobster, conch, and fish recover from overfishing, ... , The reporttitled "Review of the Benefits of ... from no-take areas around the world. The report ... expert in marine protected areas and fisheries management. ...
(Date:7/11/2014)... 11, 2014--Researchers at the Department of Energy,s Oak ... awards, presented by R&D Magazine in recognition of ... awards recognize the tremendous value of our National ... and development at the National Labs continues to ... pursue the scientific and technological innovations necessary to ...
(Date:7/11/2014)... eastern New South Wales, Australia when NASA,s Aqua satellite ... on July 11 (12:35 p.m. local time/11:35 p.m. EDT ... South Wales (NSW), the Moderate Resolution Imaging Spectroradiometer (MODIS) ... and spotted smoke (light brown) from various fires. Actively ... in red. , The New South Wales, Australia Government ...
Breaking Biology News(10 mins):Belize's lobster, conch, and fish populations rebuild in no-take zones 2ORNL wins eight R&D 100s 2ORNL wins eight R&D 100s 3ORNL wins eight R&D 100s 4
... find your location, the brain has an internal system for ... surroundings. A new study from researchers at Princeton University ... study, published in the journal Nature , indicates that ... activity up and down by working together in a collective ...
... , , , , , , ... AUDIO: Kathryn E. Henderson, PhD, Director of School and Community Initiatives at the Yale Rudd Center, talks about the first ... for more information. , , , , ... , , , , , , , ...
... 6, 2013) -- Working with a multidisciplinary consortium of ... critical information to identify CALHM1, a channel in the ... in the process of sweet, bitter, and umami (savory) ... reach the tongue, they activate taste receptors in specialized ...
Cached Biology News:Researchers discover workings of brain's 'GPS system' 2Stronger support needed for healthy beverage practices in child care 2Stronger support needed for healthy beverage practices in child care 3Stronger support needed for healthy beverage practices in child care 4Monell scientists help identify a missing link in taste perception 2
(Date:7/10/2014)... 2014 EvoDerma ’s NOOME Anti-Aging Motion ... device now comes with a second treatment cup to help ... The new cup is thinner on the edges for a ... rough, thin and uneven surfaces on the face, neck and ... stimulating treatment on areas such as the cheeks and neck, ...
(Date:7/10/2014)... DALLAS , July 10, 2014  Kainos Capital, ... consumer brands, today announced that it has acquired the ... stake in the business. Terms of the transaction were ... loss and meal replacement business that markets ready-to-drink shakes, ... North America and in the ...
(Date:7/10/2014)... -- According to the International Atomic Energy Agency ... from terrorists acquiring sufficient quantities of plutonium or ... nuclear explosive device. The IAEA also notes that ... gram-level quantities, which can be challenging to detect ... new study appearing this week in the ...
(Date:7/10/2014)... disorders has been extremely challenging because there are many ... to cause disease. Now investigators reporting in the July ... Stem Cell describe a strategy that may help ... risk factors or environmental exposures to affect the development ... variant that may predispose individuals to schizophrenia. , ...
Breaking Biology Technology:Introducing a New Bonus Treatment Cup for EvoDerma’s NOOME Anti-Aging Motion 2Kainos Capital Acquires Slim-Fast From Unilever 2Sensitive detection method may help impede illicit nuclear trafficking 2New strategy could uncover genes at the root of psychiatric illnesses 2
... Monogram announces availability of HERmark and HER1 and HER3 ... Assays ... June 2 Monogram,Biosciences, Inc. (Nasdaq: MGRM ) ... on HERmark(TM),the company,s novel oncology assay, as a means ...
... (TSH),Canada,s largest urban community hospital, has selected Chyma(TM) ... The,implementation of Chyma(TM) for their team of more ... departments at the two hospital campuses and will,soon ... "Chyma(TM) reflects the commitment of the Scarborough ...
... 2 The following release was,issued today by Thomas P. ... the New York State Common Retirement Fund, the Lead Plaintiff,in ... UNITED STATES DISTRICT COURT NORTHERN DISTRICT OF CALIFORNIA ... MCK ) SECURITIES LITIGATION, Master File No. 99-CV-20743 ...
Cached Biology Technology:Monogram Announces Presentation of Clinical Data for the HERmark(TM) Breast Cancer Assay at the 44th ASCO Annual Meeting 2Monogram Announces Presentation of Clinical Data for the HERmark(TM) Breast Cancer Assay at the 44th ASCO Annual Meeting 3Monogram Announces Presentation of Clinical Data for the HERmark(TM) Breast Cancer Assay at the 44th ASCO Annual Meeting 4Monogram Announces Presentation of Clinical Data for the HERmark(TM) Breast Cancer Assay at the 44th ASCO Annual Meeting 5Monogram Announces Presentation of Clinical Data for the HERmark(TM) Breast Cancer Assay at the 44th ASCO Annual Meeting 6Canada's Largest Urban Community Hospital Chooses Chyma(TM) On-Call Physician Scheduling Technology 2Notice of Initial Distribution of Net Settlement Funds 2Notice of Initial Distribution of Net Settlement Funds 3
DNA Polymerase I Large (Klenow) Fragment is a DNA polymerase that lacks the 5'Cut Site3' exodeoxyribonuclease activity of intact DNA Polymerase I but it contains the 3'Cut Site5' exodeoxyribonuclease...
Guanylate Kinase Purified Anti-Mouse, Anti-Rat clone 28, Isotype Mouse IgG 1 , 50 µg Consult technical datasheet for details....
Proliferin (C-14)...
... your ValveBank system! Program your valve ... computer using graphic 'click-and-drag' timebars. Save and ... program listings, then download your programs into ... EasyCode is used for programming before -- ...
Biology Products: