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Genome of CHO-K1 provides new insights into optimization of biopharmaceutical protein production
Date:8/1/2011

a and developing novel selectable markers. With the advanced genome sequencing capability of BGI, the CHO-K1 ancestral cell line was sequenced by de novo sequencing and then assembled by BGI's Short Oligonucleotide Analysis Package, SOAPdenovo, resulting in the assembly of 2.45 Gb of the genomic sequence. This information was combined with transcriptome sequence data and led to the generation of 24,383 predicted genes.

"After working with cell culture for over 20 years, this is a dream come true," said Bernhard Palsson, Ph.D., co-founder of GT Life Sciences. "We can now hope to develop metabolic and cell line engineering procedures that approach those currently available for E. coli and yeast. This development signifies the start of genome-scale science for CHO."

"The CHO cell line genome revealed the secret of its protein synthesis, modification and viral resistance, which are very unique among other cell lines and make it the optimal protein production tool." said Xu. Because of the fact that differential glycosylation can substantially affect functional activity and immune responses, researchers carried out a genome-scale assessment of CHO-K1 genes involved in protein glycosylation pathways. They identified homologs to 99% of the human glycosylation-associated genes in the CHO-K1 genome, with 53% of them expressed. The high coverage of homologs provides a unique opportunity for glycoform manipulation in CHO cells. According to the report, the genome of CHO-K1 also provided insight into viral susceptibility genes and found that key genes associated with viral entry are not expressed in CHO-K1.

"Coupling the CHO sequence with a complete genome-scale model of CHO metabolism will prove highly productive in our ability to deliver next-generation process technologies for biopharmaceutical production in CHO and other mammalian cell lines," added Iman Famili, Ph.D., Sr. Director, Research and Development at GT Life Sciences.

"The CHO-
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Contact: Jia Liu
liujia@genomics.cn
342-874-8918
Beijing Genomics Institute
Source:Eurekalert

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