Navigation Links
Genetic material under a magnifying glass
Date:1/28/2008

This release is also available in German.

The genetic alphabet contains four letters. Although our cells can readily decipher our genetic molecules, it isnt so easy for us to read a DNA sequence in the laboratory. Scientists require complex, highly sophisticated analytical techniques to crack individual DNA codes. Volker Deckert and his team at the Institute for Analytical Sciences (ISAS) in Dortmund have recently developed a method that could provide a way to directly sequence DNA. Their process is based on a combination of Raman spectroscopy and atomic force microscopy. As reported in the journal Angewandte Chemie, Deckert and Elena Bailo have successfully analyzed DNAs closest relative, RNA.

Direct sequencing means that the letters of the genetic code are read directly, as if with a magnifying glass. A DNA or RNA strand has a diameter of only two nanometers, so the magnification must be correspondingly powerful. Deckerts team uses an atomic force microscope to achieve this degree of magnification. Steered by the microscope, a tiny, silvered glass tip moves over the RNA strand. A laser beam focused on the tip excites the section of the strand being examined and starts it vibrating. The spectrum of the scattered light (Raman spectrum) gives very precise information about the molecular structure of the segment. Each genetic letter, that is, each of the nucleic acids, vibrates differently and thus has a characteristic spectral fingerprint.

The direct resolution of individual bases has not been attainable, but is also not necessary. The tip only has to be moved over the RNA strand at intervals corresponding to about the base-to-base distance. Even if the measured data then consist of overlapped spectra from several neighboring bases, the information can be used to derive the sequence of the RNA.

If this method, known as tip-enhanced Raman spectroscopy (TERS), can be extended to DNA, it could revolutionize the decoding of genetic information. Previous methods for sequencing DNA are highly complex, work indirectly, and require a large sample of genetic material. In contrast, the TERS technique developed by Deckert directly reads the code without chemical agents or detours. It also requires only a single strand of DNA. DNA sequencing could become very simple, says Deckert, like reading a barcode at the supermarket.


'/>"/>

Contact: Volker Deckert
deckert@isas.de
49-231-139-2218
Wiley-Blackwell
Source:Eurekalert

Related biology news :

1. Search for the on switches may reveal genetic role in development and disease
2. Ecologists, material scientists pursue genetics of diatoms elegant, etched casing
3. Tiny genetic differences have huge consequences: McGill researchers
4. Genetic Alliance names Genetic Testing as its official journal
5. Should children be permitted to get genetic testing for BRCA 1/2 mutations?
6. Genetically modified carrots provide more calcium
7. UBC discovery unlocks tree genetics, gives new hope for pine beetle defense
8. New method developed to identify genetic determinants of Alzheimers disease
9. Study suggests genetic connection between short stature and arthritis
10. New genetic variants associated with lipid levels, risk for coronary artery disease
11. Scientists associate 6 new genetic variants with heart disease risk factor
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/20/2016)... June 20, 2016 Securus Technologies, a ... solutions for public safety, investigation, corrections and monitoring ... involved, it has secured the final acceptance by ... for Managed Access Systems (MAS) installed. Furthermore, Securus ... to be installed by October, 2016. MAS distinguishes ...
(Date:6/7/2016)... 7, 2016  Syngrafii Inc. and San Antonio ... that includes integrating Syngrafii,s patented LongPen™ eSignature "Wet" ... collaboration will result in greater convenience for SACU ... while maintaining existing document workflow and compliance requirements. ... Highlights: ...
(Date:6/1/2016)... , June 1, 2016 Favorable ... Election Administration and Criminal Identification to Boost Global Biometrics ... recently released TechSci Research report, " Global Biometrics Market ... Competition Forecast and Opportunities, 2011 - 2021", the global ... by 2021, on account of growing security concerns across ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... , June 23, 2016 Houston ... with the Cy-Fair Sports Association to serve as ... the agreement, Houston Methodist Willowbrook will provide sponsorship ... and connectivity with association coaches, volunteers, athletes and ... with the Cy-Fair Sports Association and to bring ...
(Date:6/23/2016)... , June, 23, 2016  The Biodesign Challenge ... envision new ways to harness living systems and biotechnology, ... Art (MoMA) in New York City ... 130 participating students, showcased projects at MoMA,s Celeste Bartos ... Paola Antonelli , MoMA,s senior curator of architecture and ...
(Date:6/23/2016)... ... June 23, 2016 , ... STACS DNA Inc., the sample tracking software company, ... Crime Laboratory, has joined STACS DNA as a Field Application Specialist. , “I ... President and COO of STACS DNA. “In further expanding our capacity as a scientific ...
(Date:6/23/2016)... BEACH, Calif. , June 23, 2016  Blueprint ... new biological discoveries to the medical community, has closed ... co-founder Matthew Nunez . "We have ... us with the capital we need to meet our ... will essentially provide us the runway to complete validation ...
Breaking Biology Technology: