Navigation Links
'Genetic biopsy' of human eggs might help pick the best for IVF
Date:10/6/2011

Given how little mRNA is present in polar bodies, the task was not easy, said Gary Wessel, professor of biology, but through a combination of clever amplification and analysis techniques by lead author and graduate student Adrian Reich and second author Peter Klatsky, the team got it done.

"There's no reason this should have worked, just because there was so little material," Wessel said. "Single-cell sequencing is very challenging."

To hedge their bets the team analyzed most of their samples in two pools of 10 cells each, for instance comparing the mRNA in 10 eggs with the mRNA in the 10 related polar bodies. But to their pleasant surprise, they were also able to sequence two individual eggs and their polar bodies directly.

What they found is that more than 14,000 genes can be expressed in the eggs. Of those, more than 90 percent of the genes detected in the polar bodies were also detected in the eggs and of the 700 most abundant genes found in the polar bodies, 460 were also among the most abundant in the eggs.

Toward clinical use

"It seems that the polar body does reflect what is in the egg," Carson said. "Because the egg is the major driver of the first three days of human embryo development, what we find in the polar body may give us a clue into what is happening during that time."

But Carson and Wessel acknowledged that more research will be required to create a clinically useful tool.

Finding which genes affect embryo viability is the next major step. With the new knowledge and techniques developed in their study, the researchers said, scientists could analyze the mRNA from polar bodies of eggs that are fertilized and track the progress of the resulting embryos. Once the key genes are known, they could create fast assays to look for those genes in polar bodies so that clinicians and patients could pick the best eggs. A sufficiently developed technology could also be used for choosing which
'/>"/>

Contact: David Orenstein
david_orenstein@brown.edu
401-863-1862
Brown University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Rare genetic disorder gives clues to autism, epilepsy, mental retardation
2. Genetic Engineering & Biotechnology News reports on growing role of molecular diagnostics
3. Study finds genetic variant plays role in cleft lip
4. Genetic finding implicates innate immune system in major cause of blindness
5. American College of Medical Genetics receives $13.5M NIH contract
6. Clue to genetic cause of fatal birth defect
7. Can genetic information be controlled by light?
8. The American Society of Human Genetics hosts 58th Annual Meeting in Philadelphia
9. Modern genetics vs. ancient frog-killing fungus
10. Genetic based human diseases are an ancient evolutionary legacy
11. Genetic evidence for avian influenza movement from Asia to North America via wild birds
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/18/2014)... 18, 2014 New research into the Crimean-Congo hemorrhagic ... severe hemorrhagic disease in humans similar to that caused ... CCHFV infection. This discovery has the potential to lead ... , The research, reported in a paper ... and conducted by scientists at the Texas Biomedical Research ...
(Date:9/18/2014)... International Space Station ever starts a "frequent flyers" program, ... Recently the orbiting laboratory has hosted increasing numbers of ... from the space station in April, and another is ... experiment is planned to launch in December. , Fruit ... the world-- and in space. Model organisms can reveal ...
(Date:9/18/2014)... language is unique to humans. "The genetic changes that ... evolution to make this possible are largely unknown, but ... says Wolfgang Enard, Professor of Anthropology and Human Biology ... biological basis of language Enard has now taken an ... undertaken in collaboration with scientists at several universities, including ...
Breaking Biology News(10 mins):Research milestone in CCHF virus could help identify new treatments 2Buzzing with activity: Fruit flies orbit Earth for science 2Buzzing with activity: Fruit flies orbit Earth for science 3Buzzing with activity: Fruit flies orbit Earth for science 4Language evolution: Quicker on the uptake 2Language evolution: Quicker on the uptake 3
... University of Texas Medical Branch at Galveston have identified women ... acetate, more commonly known as Depo-Provera or the birth control ... use DMPA will gain weight and will help physicians to ... 5 percent within the first six months of use, called ...
... research shows that training your brain may be just as ... injuries, and suggests a shift from performance-based to prevention-based athletic ... is one of the four major ligaments of the knee, ... well as an economic strain on the medical system. ...
... 2009 Scientists at Burnham Institute for Medical Research (Burnham) ... a major role in promoting programmed cell death (apoptosis), helps ... and invade surrounding tissues. The study was published in the ... team of scientists, led by Kristiina Vuori, M.D., Ph.D., professor ...
Cached Biology News:UTMB study identifies women at risk of gaining excessive weight with injectable birth control 2Knee injuries may start with strain on the brain, not the muscles 2Protein that promotes cancer cell growth identified 2
(Date:9/19/2014)... DIEGO , Sept. 19, 2014 Pfenex ... in the development of high-value and difficult to manufacture ... be presenting at the 21 st Annual NewsMakers ... York . Bertrand Liang , chief executive ... company,s development programs and business strategy on Friday, September ...
(Date:9/19/2014)... Barnhardt Manufacturing Company has ... Organic Cotton . The USDA Certified Biobased Product ... ingredients meets or exceeds levels set by USDA. Biobased ... or in significant part of agricultural, forestry, or marine ... cotton to be immediately identified as a USDA certified ...
(Date:9/19/2014)... -- Dublin ... the addition of the  "Micro Market Monitor : ...      (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , , ,The ... segments in the chromatography market. The market was ... to reach $2.0 billion by 2019, at a ...
(Date:9/19/2014)... 2014 The new , Healthcare and ... available today on PharmaBoardroom.com for free download ... rocked the Spanish economy in 2009, and the austerity measures ... remained pragmatic in times of trouble. "Despite the ... biggest European market, and the general conditions to invest are ...
Breaking Biology Technology:Pfenex to Present at Upcoming Industry Conference on September 26 2Barnhardt Manufacturing Company Earns USDA Certified Biobased Product Certification and Label for Organic Cotton 2Micro Market Monitor : Global Pre-packed Chromatography Columns 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 3
... Wis. - Commercial air service doesn't always ... viewed as more fundamental to enterprise than ever ... potential customers to dismiss the expenses of putting ... of Internet video and mixed-media communication tools. , ...
... a visit to the University of Wisconsin-Madison campus, former ... his name is under attack from critics who don't understand the ... at the annual Kastenmeier Lecture at the UW-Madison Law School ... Bayh-Dole Act , which gave universities the right to patent ...
... back, we took a look at the results of the National ... time, we saw that the Midwest had two states in the top ... top 20 states. I promised to circle back with you to review ... Midwest fared in this ranking. , ,The NIH falls under the ...
Cached Biology Technology:IP video goes where air travel cannot 2IP video goes where air travel cannot 3Landmark patent law under attack, Bayh says 2Landmark patent law under attack, Bayh says 3Midwest colleges strong contenders for NIH funds 2Midwest colleges strong contenders for NIH funds 3Midwest colleges strong contenders for NIH funds 4Midwest colleges strong contenders for NIH funds 5
Purified anti-mouse Tim-1...
Request Info...
Request Info...
Anti-ADP-Ribosylation Factor 1, clone 3F1 Immunogen: Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1. Available Date: 38495...
Biology Products: