Navigation Links
Genes under control
Date:3/30/2010

In order for a gene to create a protein, the gene's DNA must first be converted into what's known as messenger RNA. These RNA molecules are the instruction manuals that show the ribosomes the cell's protein factories how to build a protein. A few years ago, scientists studying bacterial cells discovered sections in certain messenger RNAs that metabolic products (metabolites) can bind to. In doing so, they induce the RNA molecule to change its spatial structure and make it possible to switch protein production on or off. For the bacteria, these sections the riboswitches provide a fast and efficient way of controlling protein synthesis. Unsurprisingly, it had previously been impossible to demonstrate the presence of such riboswitches in the chloroplasts of plant cells.

Max Planck scientists based in Golm near Potsdam were recently the first to modify and insert riboswitches into the genetic material of the chloroplast in order to control the formation of certain chloroplast proteins. The scientists smuggled a gene into the chloroplast DNA and equipped it with a riboswitch. Theophylline, a substance found in the tea plant, was used as the "switch": it has the capacity to bind to the riboswitch on the messenger RNA, thereby enabling the chloroplast ribosomes to read the RNA. "When we spray the tobacco plants with theophylline, we find that the chloroplasts form the corresponding protein. In the absence of theophylline, no protein is produced. So the theophylline riboswitch allows us to switch a gene on or off at will and see what effects result," explains Ralph Bock from the Max Planck Institute of Molecular Plant Physiology. This had previously been a difficult thing to achieve, given that the chloroplast genome contains numerous genes which are essential for survival. Switching one of these genes off permanently would result in the death of the cell, rendering it useless for further investigation.

However, studying the way chloroplasts work in more specific detail is not the only thing that can be done with the theophylline riboswitch. Riboswitches could also play an important role in the biotechnology of the future, given that chloroplasts are well-suited to the production of potential drugs. That's because each tobacco cell contains as many as 100 chloroplasts. The chloroplast genome is therefore present in many copies. As a result, it is capable of building more proteins than the DNA in the cell nucleus. By way of example, the Potsdam-based scientists modified the genes of the tobacco plant such that it was able to produce large quantities of an antibiotic in its leaves.

Chloroplasts rarely spread through pollen

Proteins could be produced in much larger quantities in genetically modified chloroplasts. In many cases, however, these foreign proteins damage cellular metabolism or photosynthesis if the cells produce them continuously. Consequently, the growth of such plants is often inhibited or extremely slow. Riboswitches could prevent that. They could be used to switch on the corresponding genes when the plant is already fully formed and about to be harvested. Foreign genes have another advantage in the chloroplasts besides this: they are inherited almost without exception through the female egg cell. It is therefore extremely rare for foreign genes to spread through the pollen of the tobacco plants.


'/>"/>

Contact: Ralph Bock
rbock@mpimp-golm.mpg.de
49-331-567-8700
Max-Planck-Gesellschaft
Source:Eurekalert

Related biology news :

1. Autism susceptibility genes identified
2. Searching for genes behind a trait
3. Genome mapping technique speeds process of finding specific genes
4. Genes may exert opposite effects in diabetes and inflammatory bowel disease
5. Study finds genes that keep watch on blood clotting time
6. A fingerprint for genes
7. New research shows genes of pregnant women and their fetuses can increase the risk of preterm labor
8. TGen becomes center of excellence for Horizon Discoverys GENESIS and X-MAN technology
9. Ability to navigate may be linked to genes, researcher says
10. Are new genes always better?
11. Genes found linked to breast cancer drug resistance could guide future treatment choices
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/27/2016)... DUBLIN , June 27, 2016 Research ... in North America 2016-2020" report to their offering. ... North America to grow at a CAGR ... has been prepared based on an in-depth market analysis with inputs ... growth prospects over the coming years. The report also includes a ...
(Date:6/22/2016)... BETHESDA, Md. , June 22, 2016  The American ... by Trade Show Executive Magazine as one of ... Summit on May 25-27 at the Bellagio in ... based on the highest percentage of growth in each of ... number of exhibiting companies and number of attendees. The 2015 ...
(Date:6/22/2016)... 22, 2016   Acuant , the ... solutions, has partnered with RightCrowd ® ... Visitor Management, Self-Service Kiosks and Continuous Workforce ... add functional enhancements to existing physical access ... venues with an automated ID verification and ...
Breaking Biology News(10 mins):
(Date:6/23/2016)...  The Prostate Cancer Foundation (PCF) is pleased to announce 24 ... for prostate cancer. Members of the Class of 2016 were selected from a ... Read More About the Class of 2016 PCF Young Investigators ... ... ...
(Date:6/23/2016)... , June 23, 2016   EpiBiome , ... secured $1 million in debt financing from Silicon Valley ... up automation and to advance its drug development efforts, ... new facility. "SVB has been an incredible ... the services a traditional bank would provide," said Dr. ...
(Date:6/23/2016)... OTTAWA, ON (PRWEB) , ... June 23, 2016 , ... ... former DNA Technical Leader at the Arkansas State Crime Laboratory, has joined STACS DNA ... joining the STACS DNA team,” said Jocelyn Tremblay, President and COO of STACS DNA. ...
(Date:6/23/2016)... , June 23, 2016 Apellis Pharmaceuticals, ... 1 clinical trials of its complement C3 inhibitor, ... and multiple ascending dose studies designed to assess ... of subcutaneous injection in healthy adult volunteers. ... either as a single dose (ranging from 45 ...
Breaking Biology Technology: