Navigation Links
GIANT-Coli: A novel method to quicken discovery of gene function

COLLEGE STATION, Aug. 6, 2008 Think researchers know all there is to know about Escherichia coli, commonly known as E. coli? Think again. "E. coli has more than four thousand genes, and the functions of one-fourth of these remain unknown," says Dr. Deborah Siegele, a biology professor at Texas A&M University whose laboratory specializes in carrying out research using the bacterium.

Harmless E. coli strains are normally found in the intestines of many animals, including humans, but some strains can cause diseases.

Siegele and her co-workers at the University of California San Francisco, Nara Institute of Science Technology and Purdue University have devised a novel method that allows rapid and large-scale studies of the E. coli genes. The researchers believe their new method, described in the current online issue of Nature Methods, will allow them to gain a better understanding of the E. coli gene functions.

The principle behind this new method is genetic interaction. Interaction between genes produces observable effects, and this allows researchers to identify the gene functions. The research team has called their new method GIANT-Coli, short for genetic interaction analysis technology for E. coli.

The team believes that its method has great potential to quicken the progress of discovering new gene functions. The use of GIANT-Coli has already allowed researchers to identify some previously unknown genetic interactions in E. coli.

To study genetic interaction, researchers need to use what they call double-mutant strains. GIANT-Coli allows large-scale generation of these double-mutant strains (high-throughput generation). And this is the first time that a high-throughput generation method for double mutants of E. coli has been developed.

Why is it so important to know the E. coli better? "Much of what we know about other bacteria, including the more dangerous ones like Vibrio cholerae, comes from our knowledge of E. coli," says Siegele. "The E. coli is a model organism."

Siegele says that GIANT-Coli can be developed to study genetic interactions in other bacteria, and because some proteins are conserved from bacteria to humans, perhaps some of the results can even be extrapolated to gene function in humans. Moreover, Siegele points out that the method has obvious application in medicine because understanding gene functions in harmful bacteria will help in developing better treatment approaches.


Contact: Dr. Deborah Siegele
Texas A&M University

Related biology news :

1. MGH researchers describe new way to identify, evolve novel enzymes
2. Muscle mass: Scientists identify novel mode of transcriptional regulation during myogenesis
3. Novel 3-D cell culture model shows selective tumor uptake of nanoparticles
4. IdentiPHI Re Launches SAFmodule Software to Secure Novell(R) Networks
5. IdentiPHI Re Launches SAFmodule Software to Secure Novell(R) Networks
6. 454 Sequencing: Science paper describes a novel, highly efficient method of sequencing ancient DNA
7. Scientists discover novel way to remove iron from ferritin
8. Identification of a novel class of (not-so) small RNAs
9. A novel way found to prevent protein plaques implicated in Alzheimers
10. Sirtris unveils promising, novel SIRT1 activators for treating diseases of aging
11. Feinstein researchers develop new genetic method and identify novel genes for schizophrenia
Post Your Comments:
(Date:11/19/2015)... 19, 2015  Based on its in-depth analysis of ... BIO-key with the 2015 Global Frost & Sullivan Award ... Sullivan presents this award to the company that has ... needs of the market it serves. The award recognizes ... expands on customer base demands, the overall impact it ...
(Date:11/17/2015)... 17, 2015 Paris from ... --> Paris from 17 th until ... biometrics innovation leader, has invented the first combined scanner in ... same scanning surface. Until now two different scanners were required: ... can capture both on the same surface. This innovation ...
(Date:11/16/2015)... 2015  Synaptics Inc. (NASDAQ: SYNA ), ... announced expansion of its TDDI product portfolio with ... and display driver integration (TDDI) solutions designed to ... TDDI products add to the previously-announced TD4300 ... resolution), and TD4322 (FHD resolution) solutions. All four ...
Breaking Biology News(10 mins):
(Date:12/1/2015)... SEATTLE , Dec. 1, 2015 Today ... headquarters in Seattle,s South Lake ... northwest corner of Mercer Street and Westlake Avenue North, ... to the Allen Institute for Brain Science and the ... Allen , philanthropist and founder of the Allen Institute. ...
(Date:12/1/2015)... , Dec. 1, 2015 Oxford Finance LLC ... to life sciences and healthcare services companies, today announced ... loan with MDRejuvena, Inc. ("the Company"). Proceeds from the ... development of the Company,s Rejuvaphyl™ and daily skincare products. ... is the MDRejuvena brand of high potency skincare products ...
(Date:12/1/2015)... , ... December 01, 2015 , ... ... announces Park NX10 SICM Module, an add-on scanning ion conductance microscopy module to ... of SICM to an AFM. , Park SICM benefits virtually all materials characterization ...
(Date:12/1/2015)... Dec. 1, 2015 Cepheid (Nasdaq: CPHD ... at the Piper Jaffray Healthcare Conference in ... Company is reaffirming its outlook for the fourth quarter ... addition to discussing longer term business model expectations. ... Officer.  "We continue to be the fastest growing company ...
Breaking Biology Technology: