Navigation Links
GEN reports on enhancing the applications of qPCR
Date:11/18/2009

New Rochelle, NY, November 18, 2009Quantitative polymerase chain reaction (qPCR) technology is experiencing a surge of interest and rapid expansion as a result of advances such as instrumentation that pushes capacity to 1,536 wells and optimization-free multiplexing, reports Genetic Engineering & Biotechnology News (GEN). The technique's ability to both detect and simultaneously quantify specific DNA sequences is increasing its use in basic research and diagnostics, according to the November 15 issue of GEN.

"With some estimates of the qPCR assay consumables market totaling over three-quarters of a billion dollars, and growing, it's obvious that this particular technology has found a solid niche in life science research," says John Sterling, Editor in Chief of GEN.

A number of companies are making important improvements to qPCR methodologies. For example, Qiagen has developed a number of technologies for multiplex PCR that eliminate the need for optimization. Its multiplex PCR technology uses a highly stringent hot-start enzyme, an ammonium-containing buffer, and a multiplex PCR-enhancing synthetic factor MP that reportedly eliminates the need for optimization because it supports macromolecular crowding. Thus it allows efficient annealing of multiple primers under identical cycling conditions.

Bio-Rad Laboratories recently introduced its iScript RT-qPCR sample-preparation reagent for isolation of total RNA and for enabling reverse transcription and real-time PCR to be performed directly from cell lysates. Company officials say that scientists can use the protocol to remove genomic DNA and stabilize RNA in as little as five minutes.


'/>"/>

Contact: John Sterling
jsterling@genengnews.com
914-740-2196
Mary Ann Liebert, Inc./Genetic Engineering News
Source:Eurekalert

Page: 1

Related biology news :

1. BIO-key Reports Second Quarter 2007 Results
2. Communication Intelligence Corporation Reports Second Quarter 2007 Financial Results
3. Emory paleontologist reports discovery of carnivorous dinosaur tracks in Australia
4. BIO-key Reports Third Quarter 2007 Results
5. WHRC releases 4 key reports
6. Rong Li Lab reports protein interactions of MAP kinase signaling pathway
7. UVA reports surprising findings related to myotonic muscular dystrophy
8. Drug aimed at 2 bioterror agents blocks live viral infection, Weill Cornell team reports
9. MIT reports new twist in microRNA biology
10. Aware, Inc. Reports Fourth Quarter and 2007 Financial Results
11. Genetic Engineering & Biotechnology News reports on advances in miRNA
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/6/2017)... LONDON , April 6, 2017 ... Control, RFID, ANPR, Document Readers, by End-Use (Transportation & ... Energy Facility, Oil, Gas & Fossil Generation Facility, Nuclear ... Healthcare, Educational, Other) Are you looking for ... Authentication sector? ...
(Date:4/5/2017)... , April 5, 2017 Today ... announcing that the server component of the HYPR platform ... for providing the end-to-end security architecture that empowers biometric ... HYPR has already secured over 15 million users ... including manufacturers of connected home product suites and physical ...
(Date:4/4/2017)... 2017   EyeLock LLC , a leader of ... States Patent and Trademark Office (USPTO) has issued U.S. ... of an iris image with a face image acquired ... company,s 45 th issued patent. ... given the multi-modal biometric capabilities that have recently come ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... (PRWEB) , ... October 11, 2017 , ... ... pathology, announced today it will be hosting a Webinar titled, “Pathology is going ... Pathology Associates , on digital pathology adoption best practices and how Proscia improves ...
(Date:10/11/2017)... ... October 11, 2017 , ... Disappearing forests and increased emissions are ... 5.5 million people each year. Especially those living in larger cities are affected by ... in one of the most pollution-affected countries globally - decided to take action. , ...
(Date:10/10/2017)... CALIF. (PRWEB) , ... October 10, 2017 , ... San ... part of its corporate rebranding initiative announced today. The bold new look is ... reach, as the company moves into a significant growth period. , It will also ...
(Date:10/10/2017)... ... October 10, 2017 , ... Dr. Bob Harman, founder and CEO of ... Club. The event entitled “Stem Cells and Their Regenerative Powers,” was ... Dr. Harman, DVM, MPVM was joined by two human doctors: Peter B. Hanson, M.D., ...
Breaking Biology Technology: