Navigation Links
GEN reports on enhancing the applications of qPCR
Date:11/18/2009

New Rochelle, NY, November 18, 2009Quantitative polymerase chain reaction (qPCR) technology is experiencing a surge of interest and rapid expansion as a result of advances such as instrumentation that pushes capacity to 1,536 wells and optimization-free multiplexing, reports Genetic Engineering & Biotechnology News (GEN). The technique's ability to both detect and simultaneously quantify specific DNA sequences is increasing its use in basic research and diagnostics, according to the November 15 issue of GEN.

"With some estimates of the qPCR assay consumables market totaling over three-quarters of a billion dollars, and growing, it's obvious that this particular technology has found a solid niche in life science research," says John Sterling, Editor in Chief of GEN.

A number of companies are making important improvements to qPCR methodologies. For example, Qiagen has developed a number of technologies for multiplex PCR that eliminate the need for optimization. Its multiplex PCR technology uses a highly stringent hot-start enzyme, an ammonium-containing buffer, and a multiplex PCR-enhancing synthetic factor MP that reportedly eliminates the need for optimization because it supports macromolecular crowding. Thus it allows efficient annealing of multiple primers under identical cycling conditions.

Bio-Rad Laboratories recently introduced its iScript RT-qPCR sample-preparation reagent for isolation of total RNA and for enabling reverse transcription and real-time PCR to be performed directly from cell lysates. Company officials say that scientists can use the protocol to remove genomic DNA and stabilize RNA in as little as five minutes.


'/>"/>

Contact: John Sterling
jsterling@genengnews.com
914-740-2196
Mary Ann Liebert, Inc./Genetic Engineering News
Source:Eurekalert

Page: 1

Related biology news :

1. BIO-key Reports Second Quarter 2007 Results
2. Communication Intelligence Corporation Reports Second Quarter 2007 Financial Results
3. Emory paleontologist reports discovery of carnivorous dinosaur tracks in Australia
4. BIO-key Reports Third Quarter 2007 Results
5. WHRC releases 4 key reports
6. Rong Li Lab reports protein interactions of MAP kinase signaling pathway
7. UVA reports surprising findings related to myotonic muscular dystrophy
8. Drug aimed at 2 bioterror agents blocks live viral infection, Weill Cornell team reports
9. MIT reports new twist in microRNA biology
10. Aware, Inc. Reports Fourth Quarter and 2007 Financial Results
11. Genetic Engineering & Biotechnology News reports on advances in miRNA
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/18/2017)... 2017 MedNet Solutions , an ... entire spectrum of clinical research, is proud to ... for the organization in terms of corporate growth, ... products and services. The company,s exceptional achievements can ... iMedNet ™ – ...
(Date:1/11/2017)... DES MOINES, Iowa , Jan. 11, 2017 /PRNewswire/ ... another industry first with the release of its patent-pending ... to quickly and reliably perform calibrations, securely upload data ... more flexibility for the customer. "Fighting drunk ... not only for the public at large, but also ...
(Date:1/4/2017)...  For the thousands of attendees at this year,s International Consumer Electronics ... and biometric measurement devices and services, will be featuring its new line ... A&D Medical,s special CES Exhibit Suite , the new upper arm ... company,s WellnessConnected product platform.  ... ...
Breaking Biology News(10 mins):
(Date:1/18/2017)... ... January 18, 2017 , ... uBiome, the leading ... by its Science Editor, Dr. Elisabeth Bik, in the December 2016 issue of ... joined uBiome in October 2016 from her previous position at Stanford University School ...
(Date:1/18/2017)... 2017 /PRNewswire/ - SQI Diagnostics Inc. (TSX-V: SQD; ... that develops and commercializes proprietary technologies and products ... today announced that Cameron Prange , President ... from its Board of Directors.  Mr. Prange,s resignation ... that have limited both his ability to act ...
(Date:1/17/2017)... 17, 2017  An international team of researchers ... St. Boniface Hospital Albrechtsen Research Centre/University of Manitoba ... unmet health need affecting nearly one in 15 ... Investigation, their results identify small molecule drugs with ... neuronal injury in animal models of metabolic, chemical ...
(Date:1/17/2017)... 17, 2017 Research and Markets has ... Diagnostics - Technologies, Markets and Companies" to their offering. ... ... remarkably during the past few years. More than 1,000 companies ... and 342 of these are profiled in the report along ...
Breaking Biology Technology: