Navigation Links
Frog-killing fungus paralyzes amphibian immune response
Date:10/19/2013

A fungus that is killing frogs and other amphibians around the world releases a toxic factor that disables the amphibian immune response, Vanderbilt University investigators report Oct. 18 in the journal Science.

The findings represent "a step forward in understanding a long-standing puzzle why the amphibian immune system seems to be so inept at clearing the fungus," said Louise Rollins-Smith, Ph.D., associate professor of Pathology, Microbiology and Immunology. Although the identity of the toxic fungal factor (or factors) remains a mystery, its ability to inhibit a wide range of cell types including cancerous cells suggests that it may offer new directions for the development of immunosuppressive or anti-cancer agents.

The populations of amphibian species have been declining worldwide for more than 40 years. In the late 1990s, researchers discovered that an ancient fungus, Batrachochytrium dendrobatidis, was causing skin infections, and the fungus is now recognized as a leading contributor to global amphibian decline.

Rollins-Smith, an immunologist, and her colleagues have been studying the immune response to the fungus for more than 10 years.

"Amphibians have excellent and complex immune systems nearly as complex as humans and they should be able to recognize and clear the fungus," she said.

In early studies, the investigators demonstrated that some frogs produce anti-microbial peptides in the skin that offer a first layer of defense against the fungus. But when the fungus gets into the layers of the skin, Rollins-Smith said, the conventional lymphocyte (immune cell)-mediated immune response should be activated to clear it.

They found in the current studies that recognition of the fungus by macrophage and neutrophil cells was not impaired.

"We think it's not a block at the initial recognition stage," Rollins-Smith said. "The macrophages and neutrophils can see it as a pathogen, they can eat it up, they can do their thing."

But during the next stage of the immune response, when lymphocytes should be activated, the fungus exerts its toxic effects. The investigators demonstrated that B. dendrobatidis cells and supernatants (the incubation liquid separated from the cells) impaired lymphocyte proliferation and induced cell death of lymphocytes from frogs, mice and humans. The toxic fungal factor also inhibited the growth of cancerous mammalian cell lines.

The toxic factor was resistant to heat and proteases (enzymes that cut proteins into pieces), suggesting that it is not a protein. It appears to be a component of the cell wall, because drugs that interfere with cell wall synthesis reduce its inhibitory activity and because the zoospore an immature form of the fungus that lacks a cell wall does not produce the factor.

The new findings suggest the possibility that toxic factors in addition to acting locally to inhibit the immune response might also get into the circulation and have neurotoxic effects, Rollins-Smith said.

"Fungal infection causes rapid behavioral changes frogs become lethargic and start to crawl out of the water suggesting that even though the fungus stays in the skin, the toxic material is having effects elsewhere."

The studies, led by graduate students J. Scott Fites and Jeremy Ramsey, could also suggest new conservation measures for species that may be medically important.

"Amphibian skin has long been favored in folklore for its medicinal properties," Rollins-Smith said. "Frogs are a rich source of potentially useful molecules that might work against human pathogens."


'/>"/>

Contact: Leigh MacMillan
leigh.macmillan@vanderbilt.edu
615-322-4747
Vanderbilt University Medical Center
Source:Eurekalert  

Related biology news :

1. Buttercups alert farmers to first signs of subarctic fungus in the UK
2. Beetle-fungus disease threatens crops and landscape trees in Southern California
3. NYBG scientists identify 81 new plant and fungus species
4. How an ancestral fungus may have influenced coal formation
5. How a common fungus knows when to attack
6. Researchers launch new Rust-Tracker to monitor deadly wheat fungus in 27 nations
7. Natural fungus may provide effective bed bug control
8. University of Tennessee researchers find fungus has cancer-fighting power
9. Fungus responsible for 5 deaths in the wake of massive tornado
10. The secret sex life of the penicillin-producing fungus could make it more productive
11. More accurate, sensitive DNA test allows early identification of fungus causing WNS
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Frog-killing fungus paralyzes amphibian immune response
(Date:4/13/2017)... MONICA, Calif. , April 13, 2017 ... New York will feature emerging and evolving ... Summits. Both Innovation Summits will run alongside the expo ... of speaker sessions, panels and demonstrations focused on trending ... coast,s largest advanced design and manufacturing event will take ...
(Date:4/11/2017)... 11, 2017 NXT-ID, Inc. (NASDAQ:   ... announces the appointment of independent Directors Mr. Robin D. ... Board of Directors, furthering the company,s corporate governance and expertise. ... Gino Pereira , ... forward to their guidance and benefiting from their considerable expertise ...
(Date:4/4/2017)... YORK , April 4, 2017   EyeLock ... today announced that the United States Patent and Trademark ... patent broadly covers the linking of an iris image ... same transaction) and represents the company,s 45 th ... latest patent is very timely given the multi-modal biometric ...
Breaking Biology News(10 mins):
(Date:8/16/2017)... 16, 2017  This year,s edition of the Inc. 5000 features a ... workforce solutions, has made the list for the third year in a ... fastest-growing private companies based on a set of quantitative metrics. In addition, ... fastest-growing companies in the Bay State . ... Inc. 5000 ...
(Date:8/15/2017)... ... August 15, 2017 , ... Nanomedical Diagnostics ... biotherapeutics development, announces the launch of the new NHS Agile biosensor chip ... binding data for a wide range of molecules, including small and large molecules, ...
(Date:8/15/2017)... (PRWEB) , ... August 15, 2017 , ... The ... time on Immuno-Oncology 360° (IO360°) programming through a series of upcoming panels and events. ... February 7-9, 2018, at The Roosevelt Hotel in New York City. , “With our ...
(Date:8/14/2017)... ... August 14, 2017 , ... ... interconnect using USB or PCI Express, announced the release of SYZYGY™, a new ... to satisfy the need for a compact, low cost, low pin-count, high-performance connectivity ...
Breaking Biology Technology: