Navigation Links
For unzipping DNA mysteries -- literally -- Cornell physicists discover how a vital enzyme works
Date:9/18/2011

ITHACA, N.Y. With an eye toward understanding DNA replication, Cornell researchers have learned how a helicase enzyme works to actually unzip the two strands of DNA. (Nature, online Sept. 18, 2011.)

At the heart of many metabolic processes, including DNA replication, are enzymes called helicases. Acting like motors, these proteins travel along one side of double-stranded DNA, prompting the strands to "zip" apart.

What had been a mystery was the exact mechanics of this vital biological process how individual helicase subunits coordinate and physically cause the unzipping mechanism.

Cornell researchers led by Michelle Wang, professor of physics and an investigator of the Howard Hughes Medical Institute (HHMI), have observed these processes by manipulating single DNA molecules to watch what happens when helicases encounter them, and how different nucleotides that fuel the reactions affect the process. For their experiments they used an E. coli T7 phage helicase, a type with six distinct subunits, which is a good representation of how many helicases work.

"This is a great demonstration of the power of single-molecule studies," said Wang, whose lab specializes in a technique called optical trapping. To record data from single molecules, the scientists use a focused beam of light to "trap" microspheres attached to the molecules.

Prior to this work, researchers from other labs had found that the nucleotide dTTP (deoxythymidine triphosphate) was a "preferred" fuel for the helicase, and that the helicase apparently wouldn't unzip DNA if ATP (adenosine triphosphate) was provided as fuel. Wang and her colleagues found this puzzling, because ATP is known to be the primary fuel molecule in living organisms.

In their latest work, they discovered that, in fact, ATP does cause unwinding, but only in the single-molecule study could they confirm this. In normal biochemical studies, ATP doesn't seem to work, because it causes helicas
'/>"/>

Contact: Blaine Friedlander
bpf2@cornell.edu
607-254-8093
Cornell University
Source:Eurekalert

Page: 1 2

Related biology news :

1. The sweet mysteries of the nervous system
2. Cold cases gone hot: Montreal researchers solve decades-old medical mysteries using genetics
3. New look at multitalented protein sheds light on mysteries of HIV
4. Extreme archaeology: Divers plumb the mysteries of sacred Maya pools
5. Mexican salamander helps uncover mysteries of stem cells and evolution
6. Unlocking mysteries of the brain with PET
7. Nitrogen mysteries in urban grasslands
8. Zoo volunteers help explain mysteries of the genome
9. Largest ever Alzheimers genome study unveils dementia mysteries
10. Why sleep? UCLA scientist delves into one of sciences great mysteries
11. Journal of General Physiology explores mysteries of TRP channels in latest Perspectives series
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/4/2014)... CITY, Nov. 5, 2014 – University of Utah engineers ... to help jet fuel produce electricity without needing to ... used to power portable electronics, off-grid power and sensors. ... today in the American Chemical Society journal ACS ... through a chemical reaction between a fuel and an ...
(Date:11/4/2014)... CORAL GABLES, Fla. (November 4, 2014) — Think about ... and ask: How do neighboring cells know that they ... cell and how do these tissues find the correct ... (UM) are answering these crucial questions. , In a ... tissues use to communicate with their surrounding neighbors, at ...
(Date:11/4/2014)... A majority of Madagascar,s 101 species of lemurs are ... for the rainforests they call home. A new study ... can have on rainforest tree populations, which raises concerns ... the region,s rich biodiversity. , A large proportion of ... Lemurs in turn disperse the seeds of their ...
Breaking Biology News(10 mins):Jet-fueled electricity at room temperature 2The inside story: How the brain and skull stay together 2Gardeners of Madagascar rainforest at risk 2Gardeners of Madagascar rainforest at risk 3
... can discover recent dramatic developments at Kilauea Volcano, who is ... helps forecast and notify emergency managers of potential floods, which ... You can also find out how the USGS is helping ... gearing-up for baseball season, learn about the evolution and importance ...
... to support what mothers everywhere have long suspected: the ... an article titled Food Restriction Compromises Immune Memory in ... Numbers, Lynn Martin and coauthors find that reduced food ... their subjects. The findings, which will be published ...
... are not only smart, but they also wage a ... Previous studies have shown that plants can sense attacks ... not been known what happens between the pathogen attacks ... study revealed a very complex process that explains how ...
Cached Biology News:USGS April 2008 science picks 2USGS April 2008 science picks 3USGS April 2008 science picks 4USGS April 2008 science picks 5USGS April 2008 science picks 6USGS April 2008 science picks 7USGS April 2008 science picks 8Feed that cold! 2Researcher discovers pathway plants use to fight back against pathogens 2
(Date:11/26/2014)... The North America Thermal Protective Clothing Market report ... of the growth trends and revenue forecasts. It ... 2018, growing at a CAGR of 7.1% from ... of the North America Thermal Protective Clothing Market ... analysis and industry segmentation supported by various tables ...
(Date:11/26/2014)... Nov. 26, 2014 The announced sales of ... company is looking to expand in non-healthcare areas, says ... healthcare market research firm says that  Siemens is the ... biennial survey of the IVD market , with 5.5 ... in its composition would likely affect IVD market shares.  ...
(Date:11/26/2014)... -- The Alliance for Safe Biologic Medicines ... physicians at the "1 ST EuropaBio – ... the Spanish Ministry of Health, Social Services and ... Spanish Bioindustry Association (ASEBIO), included regulators from the ... and rheumatology societies, representatives from a hospital pharmacist ...
(Date:11/26/2014)... 2014 The ETC (Emerging Technology ... innovation centers, announced today that applications for AccelerateBaltimore™ ... 1st. “Do you have a big idea?,” asked ... to know about it. AccelerateBaltimore helps you and ... in just 13 weeks.” Interested game changers ...
Breaking Biology Technology:The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 2The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 3Kalorama: Sale of Three Siemens Healthcare Units Raises Questions About Firm's IVD Future 2ASBM Presents European Physicians Survey at Spanish Ministry of Health 2AccelerateBaltimore Applications to Close in 6 Days 2AccelerateBaltimore Applications to Close in 6 Days 3
... March 11 Indevus,Pharmaceuticals, Inc. (Nasdaq: IDEV ) ... and Company 28th Annual Health Care Conference, on,Tuesday, March ... being held in,Boston, Massachusetts. The presentation will be web ... section of the Indevus website at, http://www.indevus.com ., ...
... Lett, CEO of The,BioAnalytics Group LLC and Ned ... Tucson Symposium on March 12-13, 2008. The,symposium, subtitled, ... Diagnostics," will be attended by hundreds of,scientists and ... Dr. Lett,s invited presentation will focus on ...
... France, March 11 Transgene,(Euronext Paris: FR0005175080) announces ... and outlook for 2008., "2007 was a ... the signing of a partnership agreement with Roche ... of diseases caused by the,HPV virus, and the ...
Cached Biology Technology:Indevus Pharmaceuticals to Present at Cowen and Company 28th Annual Health Care Conference 2Indevus Pharmaceuticals to Present at Cowen and Company 28th Annual Health Care Conference 3BioAnalytics Group CEO, Senior Scientist to Present at Tucson Symposium March 12-13 2Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 2Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 3Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 4Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 5Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 6Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 7Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 8Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 9Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 10Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 11Transgene in 2007: Partnership and Refinancing Position the Company for its Future Development 12
Request Info...
... 30 L samples, featuring 24-position turntable. Provides up to 30 minutes of walkaway time. ... analysis and record keeping. RS-232 port. ... ... ...
... mL - 25 mL pipets ,Angled acrylic pipet ... standard square or round pipet cans. Constructed of 6 ... 76 mm W x 76 mm H x 289 ... Overall dimensions: 89 mm W x 406 mm H ...
...
Biology Products: