Navigation Links
Following the sugar right from the start
Date:5/28/2010

ng agents in vivo," Bertozzi says. "It entails metabolic labeling with synthetic azido sugars that hijack glycan biosynthesis, followed by covalent chemical tagging of the azide-labeled glycans with a compound bearing both an azide-reactive group, such as DIFO, and an imaging probe."

Two years ago, Bertozzi and her group used their copper-free click chemistry technique to image cells in live zebrafish embryos, which by virtue of being transparent, are popular for scientists studying embryogenesis. Those studies revealed dramatic differences in cell-surface expression, intracellular trafficking patterns, and tissue distributions of glycans at different stages of zebrafish larval development.

"However, we were unable to detect labeled glycans in zebrafish embryos earlier than 24 hours post-fertilization," Bertozzi says. "Because many important developmental events including cell migration, tissue morphogenesis, and cell differentiation occur in the first 24 hours of zebrafish embryogenesis, and glycan biosynthesis is also known to occur within that 24 hour period, we sought to develop methods that would enable us to image the glycans early during embryogenesis."

For this study, Bertozzi and her co-authors microinjected embryos with azido sugars at the one-cell stage, allowed the zebrafish to develop, and then detected the metabolically labeled glycans with the copper-free click chemistry technique. This enabled them to image certain types of glycans as early as seven hours post-fertilization. In addition, they used a complementary, non-metabolic labeling technique to target a class of glycans that carry sialic acid, giving them simultaneous but independent ways to image two distinct classes of glycans.

Time-lapse and multicolor imaging experiments highlighted differences between the O-glycans and sialylated glycans in the cells during the gastrulation and segmentation periods of embryogenesis. The results revealed a dramatic re-or
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Ecologist finds dire devastation of snake species following floods of 93, 95
2. LSU professor studies army-ant-following birds
3. Researchers take first steps towards spinal cord reconstruction following injury
4. MNI researchers locate neurological basis of depression following sports concussion
5. When following the leader can lead into the jaws of death
6. Following traumatic brain injury, balanced nutrition saves lives
7. Researchers block damage to fetal brain following maternal alcohol consumption
8. Following the leader can be a drag, according to students research on flapping flags
9. Following the dietary guidelines may slow heart disease in women
10. Study finds women slightly more likely to die than men in the 30 days following a heart attack
11. New study finds way to stop excessive bone growth following trauma or surgery
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Following the sugar right from the start
(Date:9/16/2014)... Columbia Center for Children,s Environmental Health at the Mailman ... an association between childhood asthma and prenatal exposure to ... products. Results appear online in the journal Environmental ... exposed during pregnancy to higher levels of the chemicals, ... 72 percent and 78 percent increase in risk of ...
(Date:9/16/2014)... September 13, 2014. The fire is human-caused and ... acres in size and is mostly being fueled by ... Monday night due to higher humidity. Yesterday (8/15), ... to construct a fireline on the West and North sides ... the fire: , 7 20-person Crews, 4 Hot Shot Crews, ...
(Date:9/16/2014)... diameter chunk of rock hit the Yukatan peninsula near the ... of 100 teratons of TNT. It left a crater more ... earthquakes and volcanism are widely accepted to have wiped out ... mammals. But what happened to the plants on which the ... the University of Arizona reveals that the meteorite impact that ...
Breaking Biology News(10 mins):Phthalates heighten risk for childhood asthma 2Phthalates heighten risk for childhood asthma 3Meteorite that doomed the dinosaurs helped the forests bloom 2Meteorite that doomed the dinosaurs helped the forests bloom 3
... 19, 2013) Columbia University College of Physicians ... announced that the Mortimer D. Sackler, M.D. Prize ... awarded to Carla Shatz, PhD, the Sapp Family ... Palo Alto, California. Dr. Shatz,s work has advanced ...
... Researchers at Plymouth University, UK, believe that findings from ... between the Early Jurassic era of 180 million years ... Through geology and palaeontology, they,ve shown how higher ... marine communities, and that while the Jurassic seas eventually ...
... elementary school students are expected to attend an INSECT EXPO ... PM on Monday, February 25 at the start of the ... Society of America (ESA), which will be held February 25-28 ... This free educational event will reinforce students, science ...
Cached Biology News:Pioneer in neural development honored with the Mortimer D. Sackler, M.D. Prize 2Pioneer in neural development honored with the Mortimer D. Sackler, M.D. Prize 3Pioneer in neural development honored with the Mortimer D. Sackler, M.D. Prize 4Jurassic records warn of risk to marine life from global warming 2
(Date:9/18/2014)... a research lab can be pretty expensive. Now a ... has published an open-source library of designs that will ... piece of equipment: the syringe pump. , Syringe pumps ... for drug delivery or mixing chemicals in a reaction. ... dollars. , Joshua Pearce and his team of Michigan ...
(Date:9/18/2014)... for computing may have just taken an encouraging step ... led by chemist Stephen O,Brien have discovered new complex ... Combining both properties is very exciting scientifically for the ... devices that might ultimately be designed, in logic circuits ... material, however, has proved difficult until now. , Using ...
(Date:9/18/2014)... (PRWEB) September 18, 2014 Whitehouse Laboratories ... with Salem Realty Group and has acquired an additional ... within the Salem Executive Complex adjacent to its current ... held on Friday September 5th. Whitehouse Labs is in ... entire space specifically for package testing. The prime location ...
(Date:9/17/2014)... , Sept. 17, 2014 /PRNewswire/ - Oncothyreon Inc. (NASDAQ: ... to offer and sell shares of its Common Stock ... concurrent underwritten public offerings.  The Series A Convertible Preferred ... Common Stock, provided that conversion will be prohibited if, ... beneficially own more than 4.99% of the Common Stock ...
Breaking Biology Technology:Doing science just got cheaper -- and faster 2Oxides discovered by CCNY team could advance memory devices 2Whitehouse Labs Announces Package Testing Laboratory Expansion 2Whitehouse Labs Announces Package Testing Laboratory Expansion 3Whitehouse Labs Announces Package Testing Laboratory Expansion 4Oncothyreon Announces Proposed Public Offerings 2
... Madison, Wis. - MyWeather LLC has ... chief marketing officer. , ,Baird went to Carleton College ... former Wisconsin Governor Martin Schreiber. He has served as ... a major K-12 technology company; vice president of sales ...
... Doyle announced that the Department of Commerce's Technology ... seed capital to Wisconsin high-tech companies of fewer than ... GenTel BioSurfaces, Inc. , helping to finance their biochip ... a microscope slide that contain multiple testing factors for ...
... The old Institute of Paper Chemistry might have left ... can still feel the void. , ,Organizers of the ... filling that void Wednesday with the PTTC's grand opening in ... paper-producing state, rolling out more than 6.4 million tons of ...
Cached Biology Technology:Paper technology transfer center opens in Green Bay 2Paper technology transfer center opens in Green Bay 3
GOAT ANTI PPP2R5B...
... Molecular Probes Fc OxyBURST Green reagent ... Simons of Boston University to permit measurement ... internalization and the subsequent oxidative burst directly ... assay reagent consists of bovine serum albumin ...
anti-dansyl, rabbit IgG fraction *1 mg/mL* Secondary Detection; Anti-Dye and Anti-Hapten Antibodies...
MOUSE ANTI RAT RT1Bu / MOUSE Ia:BIOTIN Immunogen: Rat thymocyte membrane glycoproteins...
Biology Products: