Navigation Links
Folate mystery finally solved
Date:8/22/2007

Some biochemical processes, especially those in bacteria, have been so well studied its assumed that no discoveries are left to be made. Not so, it turns out, for Johns Hopkins researchers who have stumbled on the identity of an enzyme that had been a mystery for more than 30 years. The report appears in the May 15 issue of Structure.

It was really quite a surprise when we realized we had discovered the unknown player in how bacteria make the B vitamin folate, a player that weve known of since 1974, says study author L. Mario Amzel, Ph.D., professor and director of biophysics and biophysical chemistry at Hopkins. Basic research can be so serendipitous at times.

Amzel and colleague Maurice Bessman and their labs were in the middle of systematically characterizing how members of a family of related enzymes in bacteria can recognize specific molecules. With each family member, they isolated purified enzyme, grew crystals of pure enzyme, and figured out the enzymes 3-D structure by using techniques that use X-rays.

Armed with the 3-D structure, they then used computer modeling to analyze how the enzyme binds to and acts on another molecule, its substrate.

We still didnt know that it was anything special until Maurice started searching old publications, says study author Sandra Gabelli, Ph.D. As it turns out, Suzuki and coworkers in 1974 had published evidence of an enzyme in the bacteria E. coli with similar characteristics to ours that could initiate folate biosynthesis.

So we had to ask, Can the bacteria make folate if we remove the orf17 gene" says Amzel. Bessman and colleagues then knocked-out the gene and, predictably, the bacteria made 10 times less folate than usual.

It was such a sweet discovery, says Gabelli. Its scientific discovery the old-fashioned way, finding something we werent looking for.

The mechanics behind how bacteria make folate are of particular interest to scientists who want to design more powerful antibacterial drugs. Humans cannot make folate because they do not have any of the same molecular machinery. Therefore, its possible to design drugs that target the bacterial folate machinery that would not lead to side effects in humans.

Their discovery, says Amzel, identifies yet another potential antibacterial target. We are not in that business of drug designwere focused on the basics, figuring out how things work, he says. We do hope that others can use what we find to make new drugs.


'/>"/>
Contact: Nick Zagorski
443-287-2251
Johns Hopkins Medical Institutions
Source:Eurekalert

Related biology news :

1. Scientists genetically engineer tomatoes with enhanced folate content
2. 50-year-old Mystery Solved: Protein Tags Regulate Key Ion Channel
3. Mystery Blood Vessel Disorder Implicated In Mini Strokes
4. Researchers make headway in mystery of migraines
5. Monkeypox mystery: New research may explain why 2003 outbreak in the US wasnt deadly
6. Researchers solve mystery of how nuclear pores duplicate before cell division
7. Scientists seek to unwrap the sweet mystery of the sugar coat on bacteria
8. Evolution mystery: Spider venom and bacteria share same toxin
9. Possible brain hormone may unlock mystery of hibernation
10. Mystery solved: Golds power against autoimmune diseases defined
11. ANU scientists crack DNA replication mystery
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/15/2016)... , June 15, 2016 ... market report titled "Gesture Recognition Market by Application Market - Global ... 2016 - 2024". According to the report, the  global ... billion in 2015 and is estimated to grow ... 48.56 billion by 2024.  Increasing application ...
(Date:6/7/2016)... 2016  Syngrafii Inc. and San Antonio Credit ... includes integrating Syngrafii,s patented LongPen™ eSignature "Wet" solution ... will result in greater convenience for SACU members ... maintaining existing document workflow and compliance requirements. ... Highlights: ...
(Date:6/2/2016)... YORK , June 2, 2016   The Weather ... is announcing Watson Ads, an industry-first capability in which consumers ... by being able to ask questions via voice or text ... Marketers have long sought ... the consumer, that can be personal, relevant and valuable; and ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... /PRNewswire/ - BIOREM Inc. (TSX-V: BRM) ("Biorem" or "the Company") ... shareholders, Clean Technology Fund I, LP and Clean Technology ... based venture capital funds which together hold approximately 59% ... diluted, as converted basis), that they have entered into ... holdings in Biorem to TUS Holdings Co. Ltd. ("TUS") ...
(Date:6/27/2016)... ... June 27, 2016 , ... Newly created 4Sight ... solutions to the healthcare market. The company's primary focus is on new product ... marketing strategies that are necessary to help companies efficiently bring their products to ...
(Date:6/24/2016)... TOKYO , June 24, 2016  Regular discussions on ... to take place between the two entities said Poloz. ... in Ottawa , he pointed to the ... and the federal government. ... Poloz said, "Both institutions have common economic goals, why not ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... the release of its second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” ... and retention in this eBook by providing practical tips, tools, and strategies for ...
Breaking Biology Technology: