Navigation Links
Flemish researchers provide the first experimental evidence of dynamic allostery in protein regulation
Date:7/9/2010

The brand-new Jean Jeener Bio-NMR Center at the VIB Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel, has already played a role in a scientific breakthrough that made it into the leading science journal Cell. Thanks to NMR technology, it is possible to determine the dynamic structure of proteins. So Flemish scientists put it to use to find out how the activity of certain proteins involved in the stress physiology of bacteria is regulated. This is a first in every way.

Proteins, major players in the body

Proteins play a major role in the billions of processes that occur in the body, including the development of muscle and skin, the digestion of food, the growth of cells and the generation of human emotions. Our cells continuously produce proteins, but how these complex molecules exactly function is by and large not well understood.

Not only the chemical composition but also the spatial structure of proteins is important for the performance of their functions. The ways in which they fold and unfold in three-dimensional space help determine the function of the molecules. So, without detailed knowledge about their structure, our understanding of their function usually remains partial. However, studying the spatial structure of proteins is anything but easy.

Determination of protein structure necessary for understanding function

NMR is a promising technique for determining the structure of proteins in solution. Unlike X-ray diffraction long the standard for determining the structure of proteins NMR equipment can provide dynamic structure information. Even vibrations and rotations of molecules on an atomic scale can be visualized. The Bio-NMR center at the VIB Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel, only opened May 7, 2010, but its 600-MHz and 800-MHz spectrometers have already helped produce a first article in a top journal.

First experimental evidence of dynamic allostery in protein regulation thanks to NMR

Regulation of gene transcription is a mechanism that allows cells to adapt to rapidly changing environmental conditions. In prokaryotes, genes are typically clustered in operons with each operon being regulated as an entity. The toxin-antitoxin (TA) system, which plays a role in stress, is one instance of this process.

Abel Garcia-Pino and his colleagues study the Phd-Doc toxin-antitoxin operon of P1 bacteriophages (small viruses) under the leadership of Remy Loris. Until now, no one has been able to explain the regulatory mechanism of this system at the molecular level. Hence, these VIB researchers are the first to demonstrate that, when Doc binds to the intrinsically unfolded C-terminus of Phd, it structures the DNA-binding domain of Phd. This type of communication process between two protein domains is called allostery. Already in the sixties allostery was generally assumed to be an important regulation mechanism in enzymes and Monod even called it the second secret of life (the first one being the genetic code). Several years ago, allostery between intrinsically unfolded protein domains became accepted, based on theoretical models, but now it has been experimentally demonstrated for the first time. The regulation mechanism presented here is new and probably also applies to other genes.

The NMR technology is the only technology that can detect and quantify folding and conformational changes in proteins while simultaneously providing detailed structural information. Besides its applications in fundamental biology, NMR is also a promising technology for the identification of therapeutic drugs.


'/>"/>

Contact: Joris Gansemans
joris.gansemans@vib.be
32-472-594-067
VIB (the Flanders Institute for Biotechnology)
Source:Eurekalert

Related biology news :

1. Researchers apply computing power to crack egg shell problem
2. Mount Sinai researchers discover new way diseases develop
3. Researchers develop drug delivery system using nanoparticles triggered by electromagnetic field
4. MSU researchers awarded $9.1 million grant to battle malaria in Malawi
5. Researchers calculate the cost of CO2 emissions, call for carbon tax
6. Researchers demystifying complex cellular communications hubs found in sensory neurons
7. Pitt researchers find new proteins that regulate blood pressure, flow
8. Rutgers researchers discover secrets of nutritious corn breed that withstands rigors of handling
9. Researchers discover trigger to early, effective antibody response
10. Hips dont lie: Researchers find more accurate technique to determine sex of skeletal remains
11. WSU researchers use super-high pressures to create super battery
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/6/2017)... RAM Group , Singaporean based ... in biometric authentication based on a novel  ... to perform biometric authentication. These new sensors are based on ... Ram Group and its partners. This sensor will have ... and security. Ram Group is a next generation ...
(Date:4/19/2017)... The global military biometrics market ... by the presence of several large global players. The ... major players - 3M Cogent, NEC Corporation, M2SYS Technology, ... 61% of the global military biometric market in 2016. ... military biometrics market boast global presence, which has catapulted ...
(Date:4/17/2017)... April 17, 2017 NXT-ID, Inc. (NASDAQ: ... the filing of its 2016 Annual Report on Form 10-K on ... ... is available in the Investor Relations section of the Company,s website ... SEC,s website at http://www.sec.gov . 2016 Year Highlights: ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... October 11, 2017 , ... A ... pregnancy rates in frozen and fresh in vitro fertilization (IVF) transfer ... age to IVF success. , After comparing the results from the fresh and ...
(Date:10/10/2017)... Poway, California (PRWEB) , ... October 10, 2017 , ... ... afternoon speaking at his local San Diego Rotary Club. The event ... San Diego, CA and had 300+ attendees. Dr. Harman, DVM, MPVM was joined ...
(Date:10/9/2017)... Oct. 9, 2017  BioTech Holdings announced today ... which its ProCell stem cell therapy prevents limb ... The Company, demonstrated that treatment with ProCell resulted ... saved as compared to standard bone marrow stem ... resulted in reduction of therapeutic effect.  ...
(Date:10/7/2017)... Phoenix, Arizona (PRWEB) , ... ... ... than 15 years’ experience providing advanced instruments and applications consulting for microscopy ... the in-house expertise in application consulting, Nanoscience Analytical offers a broad range ...
Breaking Biology Technology: