Navigation Links
Flemish researchers provide the first experimental evidence of dynamic allostery in protein regulation
Date:7/9/2010

The brand-new Jean Jeener Bio-NMR Center at the VIB Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel, has already played a role in a scientific breakthrough that made it into the leading science journal Cell. Thanks to NMR technology, it is possible to determine the dynamic structure of proteins. So Flemish scientists put it to use to find out how the activity of certain proteins involved in the stress physiology of bacteria is regulated. This is a first in every way.

Proteins, major players in the body

Proteins play a major role in the billions of processes that occur in the body, including the development of muscle and skin, the digestion of food, the growth of cells and the generation of human emotions. Our cells continuously produce proteins, but how these complex molecules exactly function is by and large not well understood.

Not only the chemical composition but also the spatial structure of proteins is important for the performance of their functions. The ways in which they fold and unfold in three-dimensional space help determine the function of the molecules. So, without detailed knowledge about their structure, our understanding of their function usually remains partial. However, studying the spatial structure of proteins is anything but easy.

Determination of protein structure necessary for understanding function

NMR is a promising technique for determining the structure of proteins in solution. Unlike X-ray diffraction long the standard for determining the structure of proteins NMR equipment can provide dynamic structure information. Even vibrations and rotations of molecules on an atomic scale can be visualized. The Bio-NMR center at the VIB Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel, only opened May 7, 2010, but its 600-MHz and 800-MHz spectrometers have already helped produce a first article in a top journal.

First experimental evidence of dynamic allostery in protein regulation thanks to NMR

Regulation of gene transcription is a mechanism that allows cells to adapt to rapidly changing environmental conditions. In prokaryotes, genes are typically clustered in operons with each operon being regulated as an entity. The toxin-antitoxin (TA) system, which plays a role in stress, is one instance of this process.

Abel Garcia-Pino and his colleagues study the Phd-Doc toxin-antitoxin operon of P1 bacteriophages (small viruses) under the leadership of Remy Loris. Until now, no one has been able to explain the regulatory mechanism of this system at the molecular level. Hence, these VIB researchers are the first to demonstrate that, when Doc binds to the intrinsically unfolded C-terminus of Phd, it structures the DNA-binding domain of Phd. This type of communication process between two protein domains is called allostery. Already in the sixties allostery was generally assumed to be an important regulation mechanism in enzymes and Monod even called it the second secret of life (the first one being the genetic code). Several years ago, allostery between intrinsically unfolded protein domains became accepted, based on theoretical models, but now it has been experimentally demonstrated for the first time. The regulation mechanism presented here is new and probably also applies to other genes.

The NMR technology is the only technology that can detect and quantify folding and conformational changes in proteins while simultaneously providing detailed structural information. Besides its applications in fundamental biology, NMR is also a promising technology for the identification of therapeutic drugs.


'/>"/>

Contact: Joris Gansemans
joris.gansemans@vib.be
32-472-594-067
VIB (the Flanders Institute for Biotechnology)
Source:Eurekalert

Related biology news :

1. Researchers apply computing power to crack egg shell problem
2. Mount Sinai researchers discover new way diseases develop
3. Researchers develop drug delivery system using nanoparticles triggered by electromagnetic field
4. MSU researchers awarded $9.1 million grant to battle malaria in Malawi
5. Researchers calculate the cost of CO2 emissions, call for carbon tax
6. Researchers demystifying complex cellular communications hubs found in sensory neurons
7. Pitt researchers find new proteins that regulate blood pressure, flow
8. Rutgers researchers discover secrets of nutritious corn breed that withstands rigors of handling
9. Researchers discover trigger to early, effective antibody response
10. Hips dont lie: Researchers find more accurate technique to determine sex of skeletal remains
11. WSU researchers use super-high pressures to create super battery
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/16/2017)... 16, 2017  Veratad Technologies, LLC ( www.veratad.com ), ... and identity verification solutions, announced today they will participate ... May 15 thru May 17, 2017, in ... Trade Center. Identity impacts the lives ... today,s quickly evolving digital world, defining identity is critical ...
(Date:4/18/2017)... a global expert in SoC-based imaging and computing solutions, has developed ... the company,s hybrid codec technology. A demonstration utilizing TeraFaces ® , ... showcased during the upcoming Medtec Japan at Tokyo Big Sight April ... Vegas Convention Center April 24-27. ... Click here for an image of the M820 ...
(Date:4/11/2017)... Research and Markets has announced the addition of the "Global ... ... at a CAGR of 30.37% during the period 2017-2021. ... based on an in-depth market analysis with inputs from industry experts. ... the coming years. The report also includes a discussion of the ...
Breaking Biology News(10 mins):
(Date:9/22/2017)... ... September 22, 2017 , ... ... is now being assessed in clinical trials in the United States. (clinicaltrials.gov : ... your nurse or physician or find your nearest participating clinic here https://factor-therapeutics.com/clinical-trials/ ...
(Date:9/21/2017)... , ... September 21, 2017 , ... Dr. Greg Leyer, ... Vegas, NV on September 27th. His presentation is at 12:10pm in the Probiotics Resource ... to present at SupplySide West and discuss how probiotics have shown impressive data in ...
(Date:9/21/2017)... ... September 21, 2017 , ... Colpitts Clinical has launched the ... includes new features that facilitate streamlined and compliant communication, file sharing and document ... forefront of medical advancements, they rely on efficiencies to help them address the ...
(Date:9/21/2017)... New Jersey (PRWEB) , ... September 21, 2017 ... ... highly-regulated pharmaceutical and clinical research sector professionals, has announced the addition of 5 ... Part 11 training - Compliance with Regulation 21 CFR Part 11 on ...
Breaking Biology Technology: