Navigation Links
Flemish researchers provide the first experimental evidence of dynamic allostery in protein regulation

The brand-new Jean Jeener Bio-NMR Center at the VIB Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel, has already played a role in a scientific breakthrough that made it into the leading science journal Cell. Thanks to NMR technology, it is possible to determine the dynamic structure of proteins. So Flemish scientists put it to use to find out how the activity of certain proteins involved in the stress physiology of bacteria is regulated. This is a first in every way.

Proteins, major players in the body

Proteins play a major role in the billions of processes that occur in the body, including the development of muscle and skin, the digestion of food, the growth of cells and the generation of human emotions. Our cells continuously produce proteins, but how these complex molecules exactly function is by and large not well understood.

Not only the chemical composition but also the spatial structure of proteins is important for the performance of their functions. The ways in which they fold and unfold in three-dimensional space help determine the function of the molecules. So, without detailed knowledge about their structure, our understanding of their function usually remains partial. However, studying the spatial structure of proteins is anything but easy.

Determination of protein structure necessary for understanding function

NMR is a promising technique for determining the structure of proteins in solution. Unlike X-ray diffraction long the standard for determining the structure of proteins NMR equipment can provide dynamic structure information. Even vibrations and rotations of molecules on an atomic scale can be visualized. The Bio-NMR center at the VIB Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel, only opened May 7, 2010, but its 600-MHz and 800-MHz spectrometers have already helped produce a first article in a top journal.

First experimental evidence of dynamic allostery in protein regulation thanks to NMR

Regulation of gene transcription is a mechanism that allows cells to adapt to rapidly changing environmental conditions. In prokaryotes, genes are typically clustered in operons with each operon being regulated as an entity. The toxin-antitoxin (TA) system, which plays a role in stress, is one instance of this process.

Abel Garcia-Pino and his colleagues study the Phd-Doc toxin-antitoxin operon of P1 bacteriophages (small viruses) under the leadership of Remy Loris. Until now, no one has been able to explain the regulatory mechanism of this system at the molecular level. Hence, these VIB researchers are the first to demonstrate that, when Doc binds to the intrinsically unfolded C-terminus of Phd, it structures the DNA-binding domain of Phd. This type of communication process between two protein domains is called allostery. Already in the sixties allostery was generally assumed to be an important regulation mechanism in enzymes and Monod even called it the second secret of life (the first one being the genetic code). Several years ago, allostery between intrinsically unfolded protein domains became accepted, based on theoretical models, but now it has been experimentally demonstrated for the first time. The regulation mechanism presented here is new and probably also applies to other genes.

The NMR technology is the only technology that can detect and quantify folding and conformational changes in proteins while simultaneously providing detailed structural information. Besides its applications in fundamental biology, NMR is also a promising technology for the identification of therapeutic drugs.


Contact: Joris Gansemans
VIB (the Flanders Institute for Biotechnology)

Related biology news :

1. Researchers apply computing power to crack egg shell problem
2. Mount Sinai researchers discover new way diseases develop
3. Researchers develop drug delivery system using nanoparticles triggered by electromagnetic field
4. MSU researchers awarded $9.1 million grant to battle malaria in Malawi
5. Researchers calculate the cost of CO2 emissions, call for carbon tax
6. Researchers demystifying complex cellular communications hubs found in sensory neurons
7. Pitt researchers find new proteins that regulate blood pressure, flow
8. Rutgers researchers discover secrets of nutritious corn breed that withstands rigors of handling
9. Researchers discover trigger to early, effective antibody response
10. Hips dont lie: Researchers find more accurate technique to determine sex of skeletal remains
11. WSU researchers use super-high pressures to create super battery
Post Your Comments:
(Date:9/28/2015)... 2015 Synaptics Inc. (NASDAQ: SYNA ), ... that Lenovo has selected Synaptics , Natural ID ... latest smartphone, the Vibe P1. The new Vibe ... device and provide swift access to applications and mobile ... ID FS4202 sensor solution utilizes AES256-bit encryption of the ...
(Date:9/24/2015)... , Sept. 24, 2015  EyeLock LLC, a ... showcasing its award winning and latest technology in Booth ... Anaheim, California . EyeLock,s iris authentication ... with unmatched biometric accuracy, making it the most proven ... platform uses video technology to deliver a fast and ...
(Date:9/10/2015)... This report provides detailed descriptions of the sensor types ... types that will dominate in the future. Many product ... technology hype curve in the last five years before ... all of them is the prominence of sensor options ... Sensors collect data about the physical and chemical properties ...
Breaking Biology News(10 mins):
(Date:10/13/2015)... -- PharmaCyte Biotech, Inc. (OTCQB: PMCB) shares closed at $.1035, ... average with 1,308,352 shares being traded. PMCB shares have been ... low of $.072 in September, then bouncing back to current ... due diligence will show investors in PharmaCyte Biotech, Inc. information ... using the link below at no cost. ...
(Date:10/13/2015)... ... October 13, 2015 , ... ... and assessment of organotypic 3D cell culture models, has launched a 14 ... in their patent-pending 3D InSightâ„¢ Human Liver Microtissues. The service streamlines toxicity ...
(Date:10/13/2015)... , ... October 13, 2015 , ... ... President. Steve joins as the Pistoia Alliance continues to drive to increase the ... non-profit Pistoia Alliance is focusing its vision and expanding its work in supporting ...
(Date:10/13/2015)... Oct. 13 2015 Research and Markets( ... & Europe Markets for Bone Morphogenetic Protein Growth Factor ... offering. --> --> ... the formation of bone after a fracture. In nature, ... in the formation of the skeleton. There are twenty ...
Breaking Biology Technology: