Navigation Links
First 3-D movies of living sperm
Date:2/11/2014

WASHINGTON, Feb. 11, 2014To improve their chances of success, in vitro fertilization (IVF) clinics need to assess the viability of the sperm they use. Now doctors may soon have a new technique to help them sort the good sperm cells from the less viable ones: a tracking system, developed by a team of researchers from four European institutions, that takes 3-D movies of living sperm. In addition to showing the sperm's movement and behavior in real time, the novel method simultaneously provides detailed 3-D imaging of the sperm's form and structure to detect potential infertility-causing anomalies, such as the "bent tail" that prevents the cells from swimming straight.

The researchers say this is the first technique for collecting data on sperm cell motilitya key predictor of IVF successin three dimensions and over time. They describe their method in a paper published today in The Optical Society's (OSA) open-access journal Biomedical Optics Express.

Currently, sperm concentration and mobility in semen are assessed either by subjective visual evaluation or a process known as computer-assisted sperm analysis (CASA). While the latter provides more detail and fewer errors than the former, CASA still only allows tracking and imaging in two dimensions. In their new technique, the team of researchers from Italy and Belgium combined microscopy and holographythe creation of 3-D imagesto visualize live sperm in not only two dimensions (the x and y positions) but according to their depth (z position) as well.

And, "by acquiring a video of the moving sperm in 3-D, we add a fourth dimension time," said lead author Giuseppe Di Caprio of the Institute for Microelectronics and Microsystems of the National Research Council (NRC) in Naples, Italy, and Harvard University in Cambridge, Mass.

To achieve their new tracking system, the researchers first separated laser light into two beams. They transmitted one beam through a dish containing live, swimming sperm cells and then recombined it, after magnification through a microscope, with the second beam.

"The superimposed beams generate an interference pattern that we can record on camera," Di Caprio explained. "The resulting image is a hologram containing information relative to the morphologies of the sperm and their positioning in three-dimensional space. Viewing a progressive series of these holograms in a real-time video, we can observe how the sperm move and determine if that movement is affected by any abnormalities in their shape and structure."

Di Caprio says that the 3-D imaging technique, known formally as digital holographic microscopy (DHM), yields morphology and motility data on sperm consistent with that found in previous studies, but with the unprecedented bonus of seeing cause and effect relationships between the two.

"For example," Di Caprio said, "we found that most of the sperm cells we observed swim along in one plane as expected. However, with the more detailed analysis provided by DHM, we also were able to show that this 'in-plane' movement which we believe is linked to higher potential for fertilitydoes not occur when there are morphological anomalies such as sperm with misshapen heads or 'bent tails.'"

Now that the efficiency of sperm tracking via DHM has been demonstrated, Di Caprio says that the international research team will next attempt to exploit its capabilities for defining the best-quality sperm for IVF.

"In one future experiment, we want to study sperm cells with vacuolesenclosed compartments filled with water plus organic and inorganic moleculesthat rest on the cell surface," he said. Using DHM to assess the affected sperms' motility, the team can determine if having vacuoles results in reduced fertility.

Di Caprio says that the long-term goal of such experiments and others using the new tracking system is to use the information gathered to develop a microchip-scale method for sorting good sperm cells from ones that are less viable.


'/>"/>

Contact: Angela Stark
astark@osa.org
202-416-1443
The Optical Society
Source:Eurekalert  

Related biology news :

1. Nanomotors are controlled, for the first time, inside living cells
2. Intervention in first 1,000 days vital to fulfilling childhood potential
3. Where do lizards in Qatar live? First distribution maps for the state
4. First live births with a novel simplified IVF procedure
5. Finding Israels first camels
6. CU-Boulder researchers sequence worlds first butterfly bacteria, find surprises
7. Blood and lymphatic capillaries grown for the first time in the lab
8. Asias first under-1-roof Nutritional Research Centre set up in Singapore
9. War on lionfish shows first promise of success
10. McClelland and Spelke awarded first NAS Prizes in psychological and cognitive sciences
11. First comprehensive test to detect genetic modification in food
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
First 3-D movies of living sperm
(Date:5/3/2016)... 2016  Neurotechnology, a provider of high-precision biometric ... Biometric Identification System (ABIS) , a complete system ... ABIS can process multiple complex biometric transactions with ... fingerprint, face or iris biometrics. It leverages the ... MegaMatcher Accelerator , which have been used ...
(Date:4/26/2016)... Research and Markets has announced ... 2016-2020"  report to their offering.  , ,     (Logo: ... analysts forecast the global multimodal biometrics market to ... period 2016-2020.  Multimodal biometrics is being ... the healthcare, BFSI, transportation, automotive, and government for ...
(Date:4/14/2016)... AVIV, Israel , April 14, 2016 ... Behavioral Authentication and Malware Detection, today announced the appointment ... already assumed the new role. Goldwerger,s leadership ... BioCatch, on the heels of the deployment of its ... addition, BioCatch,s behavioral biometric technology, which discerns unique cognitive ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, ... microbial test has received AOAC Research Institute approval 061601. , “This is another ... year,” stated Bob Salter, Vice President of Regulatory and Industrial Affairs. “The Peel ...
(Date:6/23/2016)... ... June 23, 2016 , ... STACS DNA Inc., the sample ... the Arkansas State Crime Laboratory, has joined STACS DNA as a Field Application Specialist. ... said Jocelyn Tremblay, President and COO of STACS DNA. “In further expanding our capacity ...
(Date:6/23/2016)... 2016 On Wednesday, June 22, 2016, ... 0.22%; the Dow Jones Industrial Average edged 0.27% lower to ... down 0.17%. Stock-Callers.com has initiated coverage on the following equities: ... (NASDAQ: NKTR ), Aralez Pharmaceuticals Inc. (NASDAQ: ... BIND ). Learn more about these stocks by accessing their ...
(Date:6/23/2016)... India , June 23, 2016 ... media market research report to its pharmaceuticals section ... profiles, product details and much more. ... spread across 151 pages, profiling 15 companies and ... available at http://www.reportsnreports.com/reports/601420-global-cell-culture-media-industry-2016-market-research-report.html . ...
Breaking Biology Technology: