The findings are detailed in a research paper that will appear online April 28 in the journal PLoS Biology, published by the Public Library of Science, a nonprofit organization of scientists and physicians. The paper's lead author was Chuan Xiao, a former Purdue postdoctoral research associate and now an assistant professor in the Department of Chemistry at the University of Texas at El Paso.
Researchers had previously been unable to determine the virus's structure because they had assumed that, like many other viruses, it's capsid possessed a design known as icosahedral symmetry.
Xiao discovered the true structure when he decided to try reconstructing the virus assuming it possessed not standard icosahedral symmetry but another configuration called five-fold symmetry.
"If you start out thinking the object has icosahedral symmetry, then you assume there are 60 identical pieces, and that influences how you reconstruct the virus's structure," Rossmann said.
Researchers took images of the virus using an atomic force microscope, revealing a pattern of holes regularly spaced throughout the virus's outer shell.
"The capsids of most other large, pseudo icosahedral viruses do not contain such holes, and their function is unknown," Rossmann said.
The researchers used a method called cryo-electron microscopy reconstruction to determine the structure details. The reconstruction method enables researchers to produce three-dimensional structures by combining two-dimensional pictures, much like a complete architectural drawing of a house can be assembled with two-dimensional drawings of the sides, the roof and other elements.
Using five-fold symmetry rev
|Contact: Emil Venere|