Navigation Links
Findings show insulin -- not genes -- linked to obesity
Date:4/14/2009

rofit organization of scientists and physicians.

"This varied capability to store fat among genetically identical cells is a well-observed but poorly understood phenomenon," Cheng said

The researchers determined that these differences in fat storage depend not on fat-gene expression but on variations in a cascade of events within an "insulin-signaling pathway." The pathway enables cells to take up glucose from the blood.

"Only one small variation at the beginning of the cascade can lead to a drastic variation in fat storage at the end of the cascade," Cheng said.

The researchers conducted "single cell profiling" using a combination of imaging techniques to precisely compare fat storage in cloned cells having the same fat genes expressed.

Single cell profiling allows researchers to precisely compare the inner workings of individual cells, whereas the conventional analytical approach in biochemistry measures entire populations of cells and then provides data representing an average.

"In this case, we don't want an average. We need to find out what causes fat storage at the single-cell level so that we can compare one cell to another, " Le said. "By profiling multiple events in single cells, we found that variability in fat storage is due to varied rates of insulin processing among cells."

The cell culture used in the research contains cloned mice fibroblast cells.

"This particular type of cell culture has been used to study the molecular control of obesity for the past 35 years," Cheng said. "Researchers have observed tremendous variability in how much fat is stored in cells with identical genes, but no one really knows why. Our findings have shed some light on this phenomenon."

The researchers used a specialized imaging method called coherent anti-Stokes Raman scattering, or CARS, combined with other techniques, including flow cytometry and fluorescence microscopy.

"This multimo
'/>"/>

Contact: Emil Venere,
venere@purdue.edu
765-494-4709
Purdue University
Source:Eurekalert  

2

GOOD

Page: 1 2 3

Related biology news :

1. New prostate cancer research findings
2. K-State researchers findings on E. coli
3. UVA reports surprising findings related to myotonic muscular dystrophy
4. Womens health-related scientific findings presented by University of Pittsburgh researchers
5. Findings a step toward making new optical materials
6. Pioneering landscape-scale research releases first findings
7. Findings released from 1 of the largest percutaneous coronary intervention trials ever
8. Study findings show infection control intervention helps keep kids in school
9. New findings on immune system in amphibians
10. International conference to release new findings on smell and taste
11. Researcher helping to pioneer medical therapy for Fragile X Syndrome presents latest findings
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Findings show insulin -- not genes -- linked to obesity
(Date:5/17/2013)... 2013) The AGA Research Foundation announced a new ... between the gut microbiota, one of today,s most exciting ... , The AGA Research Awards Panel selected Andrew T. ... Hospital and Harvard Medical School, Boston, as the 2013 ... will receive $25,000 of funding, commencing in July 2013, ...
(Date:5/17/2013)... the white noise of the South, but the night belongs ... air hangs heavy from the humidity and the sounds of ... species of frogs, toads and salamanders, is the center of ... swamps are the auditorium for their symphonic choruses, the scientists ... or ARMI, have front-row seats. , Amphibians, which ...
(Date:5/17/2013)... State University of New Jersey, and City University of ... Visikol TM that effectively clears organisms to be ... of chloral hydrate, which is one of the few ... regulated by the Drug Enforcement Administration (DEA) due to ... clearing agents, are vital for viewing organisms under a ...
Breaking Biology News(10 mins):New gut microbiome research to explore red meat -- colorectal cancer pathway 2Front-row seats to climate change 2Front-row seats to climate change 3Front-row seats to climate change 4New formula invented for microscope viewing, substitutes for federally controlled drug 2New formula invented for microscope viewing, substitutes for federally controlled drug 3
... signs of climate change in the upper atmosphere and ... Tyler Prize for Environmental Achievement. The award, ... will go to Richard Alley, professor of geosciences at ... atmospheric and climate sciences in the Scripps Institution of ...
... French and Spanish . , The ... projects assembled from researchers in more than 60 countries. Launched ... March 2009 to allow for observations during the alternate seasons ... ICSU, IPY spearheaded efforts to better monitor and understand the ...
... University of Delaware researchers have won the American Physical ... orthopedics published in 2008. Their study focused on ... management of anterior cruciate ligament (ACL) injuries. Each ... which is one of the major ligaments of the ...
Cached Biology News:Winners of Tyler environmental prize announced 2Polar research reveals new evidence of global environmental change 2Polar research reveals new evidence of global environmental change 3Researchers win award for best clinical paper in orthopedic physical therapy 2
(Date:5/16/2013)... 16, 2013 Scientists at the Center for ... , and the California -based biopharmaceutical ... novel therapy for reversing memory decline in mice with ... significant cognitive improvement in advanced stage AD mice. The ... reversing cognitive decline for advanced stage AD mice, and ...
(Date:5/16/2013)... 16, 2013 Reportlinker.com announces ... is available in its catalogue: ... Analysis: Therapeutic Proteins – Biosimilars, Biobetters ...   Product ... The new Competitive Intelligence Report Therapeutic ...
(Date:5/16/2013)... ON (PRWEB) May 16, 2013 DNA Genotek, ... announced ‘Spit for Africa’, a sponsorship program in conjunction with ... is designed to improve access and reduce the cost of ... projects such as the Human Heredity and Health in Africa ... the AfSHG. , The aim of the AfSHG ...
(Date:5/16/2013)... May 16, 2013 Leading UK ... Success for Companion Diagnostic–Drug Pairings in Oncology, a ... biopharmaceutical manufacturers should understand when developing new drug-test ... particularly in oncology, with two recently approved drug–test ... BRAF-mutation positive melanoma. Payors are willing to consider ...
Breaking Biology Technology:Elevation of Brain Magnesium Reverses Memory Deficits in Alzheimer Mice 2Elevation of Brain Magnesium Reverses Memory Deficits in Alzheimer Mice 3Competitor Analysis: Therapeutic Proteins - Biosimilars, Biobetters & Biosuperiors 2Competitor Analysis: Therapeutic Proteins - Biosimilars, Biobetters & Biosuperiors 3Competitor Analysis: Therapeutic Proteins - Biosimilars, Biobetters & Biosuperiors 4Competitor Analysis: Therapeutic Proteins - Biosimilars, Biobetters & Biosuperiors 5Competitor Analysis: Therapeutic Proteins - Biosimilars, Biobetters & Biosuperiors 6Competitor Analysis: Therapeutic Proteins - Biosimilars, Biobetters & Biosuperiors 7Competitor Analysis: Therapeutic Proteins - Biosimilars, Biobetters & Biosuperiors 8DNA Genotek Inc. Announces Spit for Africa Program 2DNA Genotek Inc. Announces Spit for Africa Program 3Market Access Expert Firm, PRMA Consulting, Launches Companion Diagnostics Handbook 2
... /Xinhua-PRNewswire/ -- Olympus Capital Holdings Asia and,its co-investors ... to,sell Arysta LifeScience Corporation, the world,s largest privately ... value of,approximately JPY 250 billion (US$2.2 billion). The ... a leading middle market private,equity firm with investment ...
... Calif., Oct. 22 AMDL, Inc. (Amex:,ADL), ... Shenzhen,Jiangxi and Jilin, China, through its wholly ... international biopharma company that,engages in the development, ... products. AMDL announced today that its,management will ...
... Investigating New Cases of Chagas, Disease ... Bites & Blood Transfusions, ANAHEIM, Calif., Oct. 20 ... licensed the first blood-screening test,for Chagas, disease, some 241 blood ... to the parasite known to cause,this serious and potentially fatal ...
Cached Biology Technology:Olympus Capital Announces Agreement to Sell Arysta LifeScience Corporation 2Olympus Capital Announces Agreement to Sell Arysta LifeScience Corporation 3AMDL to Present its China Success Story at Rodman & Renshaw 9th Annual Healthcare Conference 2Blood Donations in U.S. Testing Positive for Chagas' Disease 2Blood Donations in U.S. Testing Positive for Chagas' Disease 3Blood Donations in U.S. Testing Positive for Chagas' Disease 4
... Highly nutritious peptone, ... the production of bacterial ... cultivation of bacteria with ... Corynbacterium, Haemophilus, Neisseria, Pasteurella, ...
... in Minimum Essential Medium (Eagle) with 2 mM ... growth. In order to keep the antigens ... acetone-methanol. The cells are arrayed on a ... wells surface specifically treated to enhance cellular attachment ...
... cells were cultured in Ham's F12K medium with ... phase of growth. In order to keep the ... in acetone-methanol. The cells are arrayed on a ... wells surface specifically treated to enhance cellular attachment ...
... cultured in Minimum Essential Medium (Eagle) with 2 ... of growth. In order to keep the ... in acetone-methanol. The cells are arrayed on ... each wells surface specifically treated to enhance cellular ...
Biology Products: