Navigation Links
Faster Salmonella detection now possible with new technique
Date:5/4/2010

sieving them through a polymer matrix (a gel) contained in glass capillaries that are 50 microns -- not much thicker than a human hair. This process separates the DNA fragments according to their size, from smallest to largest, and each piece is detected as it passes in front of an intense light source. For a PCR product that's 300 bases long, this separation and detection process takes approximately 90 minutes.

Because the SNAP71 reagent cleaves the salmonella DNA only at adenine and guanine, and not at thymine and cytosine sites (T's and C's), the method is not a direct replacement for DNA sequencing. Instead, the process rapidly generates a reproducible pattern of DNA fragments, Brehm-Stecher said.

Salmonella strains having slightly different DNA sequences within a given gene will yield different patterns of fragments, allowing discrimination of different strains of salmonella.

From "food to finish," the whole process can be accomplished in about two and a half hours.

"We're very excited about this approach and about the rapid progress we've made since the project began," said Brehm-Stecher. "The funding for this project has enabled us to work very closely with Advanced Analytical and accelerate application of their instruments to solving important food safety problems."

The team at Iowa State University includes post doctoral researcher Hyun Jung Kim and master's student Brittany Porter. The group is also working with Cleveland Clinic in Ohio.

The ultimate goal of the project is faster detection and characterization of human pathogens from "farm to fork to physician."

Advanced Analytical's instruments are based on technology originally developed at Iowa State University in the lab of Ed Yeung, the Robert Allen Wright Professor and Distinguished Professor in Liberal Arts and Sciences and professor at the U.S. Department of Energy's Ames Lab.


'/>"/>

Contact: Byron Brehm-Stecher
byron@iastate.edu
515-294-6469
Iowa State University
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. UCLA engineers develop faster method to detect bacterial contamination in coastal waters
2. Chubby birds get there faster
3. ASU scientists develop universal DNA reader to advance faster, cheaper sequencing efforts
4. Ecologists discover forests are growing faster
5. Boston University reseachers develop faster, cheaper DNA sequencing method
6. Faster, cheaper way to find disease genes in human genome passes initial test
7. Faster, more cost-effective DNA test for crime scenes, disease diagnosis
8. Mice run faster on high-grade oil
9. The faster they come
10. New wheat disease could spread faster than expected
11. Shellfish and inkjet printers may hold key to faster healing from surgeries
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Faster Salmonella detection now possible with new technique
(Date:6/23/2015)... 22, 2015 Research ... of the "Body-Worn Temperature Sensors Market - ... Forecast 2014 - 2020" report to their ... of the global body-worn temperature sensors market. The ... on the basis of types, care setting, patient ...
(Date:6/18/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the "Company") ... market announces that its Wocket® smart wallet will be the ... scheduled to air on WABC Radio (am770) in ... The broadcast air- time for the extensive one ... Inc.,s CEO Gino Pereira will be discussing its ...
(Date:6/17/2015)... June 17, 2015  Crossmatch™, a leading provider ... its U.are.U ® 4500 fingerprint readers have ... Eateries, Inc., a San Jose, CA ... increase security, improve accountability and reduce fraud at ... non-repudiable identity confirmation for employee sign in and ...
Breaking Biology News(10 mins):Body-Worn Temperature Sensors Market - Global Industry Analysis 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 2Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 3
... If the fight against breast cancer were a criminal investigation, ... among the shadier figures. In that vein, a study to ... Chemistry is the police report that reveals a key ... nucleus, aided and abetted by the other proteins. The research ...
... Eleven years ago, the German Physical Society (DPG) has ... to promote interdisciplinary applications of physics. The Prize, endowed ... a key instrument to encourage research in this direction. ... at the Max Planck Institute for Evolutionary Biology in ...
... use circle hooks in some fishing tournaments because they ... such as marlin. However, research from North Carolina State ... impact charter and recreational fishing, since they make it ... know whether circle hooks are effective as conventional J ...
Cached Biology News:Protein 'jailbreak' helps breast cancer cells live 2'Young Scientist Award for Socio- and Econophysics 2012' for Arne Traulsen 2Study finds circle hooks lower catch rate for offshore anglers 2
(Date:6/30/2015)... ... June 30, 2015 , ... ... does not sacrifice selectivity to achieve faster flow rates in comparison to nylon ... with significant open space to allow easy liquid flow while trapping particulates as ...
(Date:6/30/2015)... ... June 30, 2015 , ... The maximum number of ... currently outstanding common shares (including shares of unvested restricted stock). On June 29, 2015, ... sale price of Cryo-Cell’s shares reported on the OTCBB was $2.29 per share. , ...
(Date:6/30/2015)... 30, 2015  The University of California, San ... and UC San Diego Extension announced their third ... Aug.  12 – 14, 2015, at UC San ... provides an intense exposure to best practices and ... well-suited to biologists, process engineers and business executives ...
(Date:6/30/2015)... /PRNewswire/ - RepliCel Life Sciences Inc. (OTCQB: REPCF) ... focused on the development of autologous cell therapies, announced ... 1/2 clinical trial of RCT-01, being tested for ... been enrolled and their tissue biopsy sent for ... comprised of non-bulbar dermal sheath (NBDS) cells isolated ...
Breaking Biology Technology:New Nanofiber Membrane Provides Fast, Efficient Filtration 2Cryo-Cell Commences Tender Offer to Purchase its Common Stock, Up to 750,000 Shares at $3.25 per Share 2Cryo-Cell Commences Tender Offer to Purchase its Common Stock, Up to 750,000 Shares at $3.25 per Share 3UC San Diego Extension to Offer Third Annual Microbial Fermentation Workshop 2RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 2RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 3RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 4
... eco-systems -- where the seeds of start-up and early ... require venture capital, high technologies, and new government programs. ... together, a national expert on entrepreneurial development told an ... presentation, Building a Community Entrepreneurial Culture, is one in ...
... Panelists at the Demo 2003 conference offered insights into the ... we look back in the year 2025 what will see ... Open Source Applications Foundation, and founder of Lotus, began the ... Bio and Nano Technology space, its where the IT industry ...
... - In this time of the chronic threat of terrorism ... be armed with biological weapons, high on the wish list ... easy way to detect the presence of dangerous biological agents. ... developed by chemists at the University of Wisconsin-Madison, the age ...
Cached Biology Technology:Entrepreneurs Dont RequireVenture capital, high-tech or government programs 2Entrepreneurs Dont RequireVenture capital, high-tech or government programs 3Industry Veterans take a look back from 2025: Bio-Technology, and Bio-Informatics are the focus 2Diamond Film May Enable Critical New Sensors For Bioterror 2Diamond Film May Enable Critical New Sensors For Bioterror 3Diamond Film May Enable Critical New Sensors For Bioterror 4
... CLS number is a new ... match Cornings product number. If ... order under the old Sigma-Aldrich ... service for assistance. ID clarifier: ...
... combines top performance with exquisite design. The ... and unparalleled temperature uniformity. The instrument has ... a spreadsheet or alternatively in a graphical ... on the push of a button and ...
Preparation TW--Compatibility: no end cap seals -Hybridization Tube for Hybrigene -Comp Dim: 240 80 mm -Material: borosilicate glass -Mfr Desig: Techne FHB-15...
... software for all your biopharmaceutical applications needs. ... single, scalable software package. The graphical user ... control of all Freedom instruments from simple ... with one liquid handling arm up to ...
Biology Products: