Navigation Links
Faster Salmonella detection now possible with new technique
Date:5/4/2010

sieving them through a polymer matrix (a gel) contained in glass capillaries that are 50 microns -- not much thicker than a human hair. This process separates the DNA fragments according to their size, from smallest to largest, and each piece is detected as it passes in front of an intense light source. For a PCR product that's 300 bases long, this separation and detection process takes approximately 90 minutes.

Because the SNAP71 reagent cleaves the salmonella DNA only at adenine and guanine, and not at thymine and cytosine sites (T's and C's), the method is not a direct replacement for DNA sequencing. Instead, the process rapidly generates a reproducible pattern of DNA fragments, Brehm-Stecher said.

Salmonella strains having slightly different DNA sequences within a given gene will yield different patterns of fragments, allowing discrimination of different strains of salmonella.

From "food to finish," the whole process can be accomplished in about two and a half hours.

"We're very excited about this approach and about the rapid progress we've made since the project began," said Brehm-Stecher. "The funding for this project has enabled us to work very closely with Advanced Analytical and accelerate application of their instruments to solving important food safety problems."

The team at Iowa State University includes post doctoral researcher Hyun Jung Kim and master's student Brittany Porter. The group is also working with Cleveland Clinic in Ohio.

The ultimate goal of the project is faster detection and characterization of human pathogens from "farm to fork to physician."

Advanced Analytical's instruments are based on technology originally developed at Iowa State University in the lab of Ed Yeung, the Robert Allen Wright Professor and Distinguished Professor in Liberal Arts and Sciences and professor at the U.S. Department of Energy's Ames Lab.


'/>"/>

Contact: Byron Brehm-Stecher
byron@iastate.edu
515-294-6469
Iowa State University
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. UCLA engineers develop faster method to detect bacterial contamination in coastal waters
2. Chubby birds get there faster
3. ASU scientists develop universal DNA reader to advance faster, cheaper sequencing efforts
4. Ecologists discover forests are growing faster
5. Boston University reseachers develop faster, cheaper DNA sequencing method
6. Faster, cheaper way to find disease genes in human genome passes initial test
7. Faster, more cost-effective DNA test for crime scenes, disease diagnosis
8. Mice run faster on high-grade oil
9. The faster they come
10. New wheat disease could spread faster than expected
11. Shellfish and inkjet printers may hold key to faster healing from surgeries
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Faster Salmonella detection now possible with new technique
(Date:8/21/2014)... National University (ANU) team has successfully replicated one of ... biological systems powered by sunlight which could manufacture hydrogen ... is sunlight. It is an exciting prospect to use ... safely," said Dr Kastoori Hingorani, from the ARC Centre ... School of Biology. , Hydrogen offers potential as a ...
(Date:8/21/2014)... pancreatitis involves the pancreas digesting itself resulting in severe ... the UK around 20,000 patients are diagnosed with the ... cure and treatment is restricted to intravenous fluid and ... Faculty of Life Sciences, who led the research, said ... from gall stones and excessive alcohol intake combined with ...
(Date:8/20/2014)... In a study of 158 pregnant teenagers in Rochester, ... and intentional consumption of ice, cornstarch, vacuum dust, baby ... new Cornell study. , Moreover, such teens had significantly ... not eat nonfood substances. , Pregnant teens, regardless of ... can lead to iron deficiency and anemia. Low iron ...
Breaking Biology News(10 mins):Water and sunlight the formula for sustainable fuel 2Insulin offers new hope for the treatment of acute pancreatitis 2Pica in pregnant teens linked to low iron 2
... of Soil, they're just clever Lab guys. A team from ... Science Magazine with a new way to count bugs in ... our feet. , "Computational Improvements Reveal Great Bacterial Diversity and ... and John Dunbar, of Los Alamos' Bioscience Division, describes a ...
... CropScience and DuPont have developed two new classes of ... more effective way to fight pest insects that damage ... compounds designed to activate a novel insecticide target called ... problem of insecticide resistance, the researchers say. They described ...
... at The Schepens Eye Research Institute have discovered that ... in the eye and other parts of the body ... body's immune response and protect delicate tissues that cannot ... believe that this discovery may ultimately help in the ...
Cached Biology News:Using computers and DNA to count bacteria 2Novel compounds show promise as safer, more potent insecticides 2A protein in the eye may prevent immune response and protect eyes from disease 2
(Date:8/21/2014)... His Majesty Willem-Alexander, King of the ... of His Majesty Willem-Alexander, King of the Netherlands, has ... of POET-DSM Advanced Biofuels’ Project LIBERTY cellulosic ethanol plant ... King will take part in the Grand Opening Ceremony ... a.m.-12:20 p.m. and tour the plant. POET-DSM is a ...
(Date:8/21/2014)... , Aug. 21, 2014  Decision Resources ... Brazil and Mexico ... uptake of several newly approved agents, the anticipated ... indication and the launch of emerging biologics will ... In particular, the emerging IL-5 inhibitors will introduce ...
(Date:8/21/2014)... , Aug. 21, 2014  China Cord Blood Corporation (NYSE: ... plan to release financial results for the first quarter ... after market close in the US.  ... a.m. ET on Friday, August 29, 2014 to discuss ... the Company,s recent developments, followed by a question and ...
(Date:8/21/2014)... 2014 Ontotext S4 , The ... Ontotext . Now the same enterprise hardened text ... and unstructured data is available to start-ups and mid-size ... technology. Organizations that do not have resources to evaluate ... use S4 since there is no need for on ...
Breaking Biology Technology:His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 2His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 3Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 2Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 3China Cord Blood Corporation to Report First Quarter of Fiscal 2015 Financial Results 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 3Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 4
... Direct, a wholly owned subsidiary of Medco Health Solutions, ... provider of guidance and decision support for genomic medicine, ... Atlantic Health of Morristown, New Jersey, Crittenton Hospital Medical ... Chicago, Illinois, have joined the Genomic Medicine Network to ...
... 31, 2011 Caliper Life Sciences, Inc. (Nasdaq: ... agreed to a long-term, exclusive arrangement under which Caliper will ... North America. With the LabChip DS, an ... analyze the concentration levels of up to 96 nucleic acid ...
... Plus is pleased to offer Clinical and Non-Clinical ... health care organizations for front line selection and ... increase patient satisfaction and employee engagement and reduce turnover ... diminished medical errors and infection rates, better team dynamics, ...
Cached Biology Technology:Four Community Hospitals Join DNA Direct's Genomic Medicine Network 2Four Community Hospitals Join DNA Direct's Genomic Medicine Network 3Caliper Life Sciences Launches Microfluidic LabChip® DS Platform for UV/VIS Spectral Analysis of Nucleic Acid and Protein Samples 2Caliper Life Sciences Launches Microfluidic LabChip® DS Platform for UV/VIS Spectral Analysis of Nucleic Acid and Protein Samples 3Talent Plus' Health Care Talent Online® Assessments 2Talent Plus' Health Care Talent Online® Assessments 3
... Using multicolor analysis in a modern flow ... cells that are differentially labeled with fluorescent ... dyes typically used for cell labeling are ... by a flow cytometers filters. Thus, the ...
... number is a new product ... Cornings product number. If showing ... under the old Sigma-Aldrich number ... for assistance. ID clarifier: rack ...
... leading provider of X-ray crystallography services ... screening services to its pharmaceutical and ... binding of drug-like fragments to the ... targets, allowing scientists to develop novel, ...
...
Biology Products: