Navigation Links
Faster CARS, less damage: NIST chemical microscopy shows potential for cell diagnostics
Date:10/14/2010

A paper by researchers at the National Institute of Standards and Technology (NIST) may breathe new life into the use of a powerfulbut trickydiagnostic technique for cell biology. The paper,* appearing this week in the Biophysical Journal, demonstrates that with improved hardware and better signal processing, a powerful form of molecular vibration spectroscopy can quickly deliver detailed molecular maps of the contents of cells without damaging them. Earlier studies have suggested that to be useful, the technique would need power levels too high for cells.

The technique, "B-CARS,"** is one of several variations on Raman spectroscopy, which measures the frequencies associated with different modes of vibration of atoms and their bonds in a molecule. The exact mix of these frequencies is an extremely discriminating "fingerprint" for any particular molecule, so Raman spectroscopy has been used as a chemical microscope, able to detail the structure of complex objects by mapping the chemical composition at each point in a three-dimensional space.

In the biosciences, according to NIST chemist Marcus Cicerone, Raman spectroscopy has been used to detect microscopic cellular components such as mitochondria, detect how stem cells differentiate into new forms and distinguish between subtly different cell and tissue types. It can, for example, detect minor differences between various precancerous and cancerous cells, potentially providing valuable medical diagnostic information. Even better, it does this without the need to add fluorescent dyes or other chemical tags to identify specific proteins.

The catch, says Cicerone, is speed. The usual method, spontaneous Raman scattering takes a long time to gather enough data to generate a single spectrumas much as seven minutes for fine detailand that's for each point in the image. "Seven minutes or even five seconds per spectrum is not feasible when we need a million spectra for an image," he observes. CARS, which uses a pair of lasers to pump up the vibrational states and increase signal, is part of the answer. The current breakthroughs for a broadband CARS instrument developed at NIST since 2004, says Cicerone, gets the same information in 50 milliseconds per pixel.

The new catch is power. Recent papers have argued that to get the necessary data, the lasers used in CARS must run at power levels above the damage threshold for living cells, making the technique nearly useless for clinical purposes. Not quite, according to the NIST team. Their paper describes a combination of improved hardware to gather spectra over a very broad range of wavelengths, and a clever mathematical technique that effectively amplifies the useable signal by examining a portion of signal normally ignored as background interference. The result, says Cicerone, pushes their minimum power level below the damage threshold while retaining the speed of CARS. "We have all the information that you have in a Raman spectrum but we get it 5 to 100 times faster," he says, adding that some obvious modifications should push that higher, opening the door to more widespread use of vibrational spectroscopy in both biology and clinical diagnosis.


'/>"/>

Contact: Michael Baum
michael.baum@nist.gov
301-975-2763
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Related biology news :

1. Emissions rising faster this decade than last
2. New type of vaccines deliver stronger and faster immune response
3. Ocean growing more acidic faster than once thought
4. New open-source software permits faster desktop computer simulations of molecular motion
5. Shellfish and inkjet printers may hold key to faster healing from surgeries
6. New wheat disease could spread faster than expected
7. The faster they come
8. Mice run faster on high-grade oil
9. Faster, more cost-effective DNA test for crime scenes, disease diagnosis
10. Faster, cheaper way to find disease genes in human genome passes initial test
11. Ecologists discover forests are growing faster
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Faster CARS, less damage: NIST chemical microscopy shows potential for cell diagnostics
(Date:12/22/2016)... MOUNTAIN VIEW, Calif. , Dec. 20, 2016  As ... all levels, 23andMe, the leading personal genetics company, recently released ... Only Me . The book focuses on the topics ... the Next Generation Science Standards (NGSS) taught in elementary school ... the second in a series by illustrator Ariana Killoran ...
(Date:12/20/2016)... and GENEVA, Dec, 20, 2016   Valencell , ... technology, and STMicroelectronics (NYSE: STM), a global ... electronics applications, announced today the launch of a ... biometric wearables that includes ST,s compact SensorTile ... Benchmark™ biometric sensor system. Together, SensorTile and ...
(Date:12/16/2016)... Research and Markets has announced the addition ... to 2021" report to their offering. ... The biometric vehicle access system market, ... of 14.06% from 2016 to 2021. The market is estimated to ... 854.8 Million by 2021. The growth of the biometric vehicle access ...
Breaking Biology News(10 mins):
(Date:1/20/2017)... Jan. 20, 2017 Ginkgo Bioworks, the ... a pioneer in the synthesis and assembly of ... in assembling pathway-length synthetic DNA into Ginkgo,s automated ... in the construction of new organism designs for ... "Gen9 was founded to significantly increase the ...
(Date:1/19/2017)... and HOUSTON , Jan. ... Prenatal") today announced the formation of its Medical/Clinical ... clinicians and industry veterans who enhance the range ... it accelerates development of its novel prenatal diagnostic ... medical, clinical and strategic guidance for the company,s ...
(Date:1/19/2017)... GAITHERSBURG, Md. , Jan. 19, 2017 /PRNewswire/ ... a privately-held immunotherapeutics company targeting infectious diseases, announced ... the merger of PharmAthene and Altimmune in an ... Fund, HealthCap, Truffle Capital and Redmont Capital. The ... immunotherapeutics company with four clinical stage and one ...
(Date:1/19/2017)... ... January 19, 2017 , ... FireflySci Inc. is a go-getter ... The tremendous growth is accounted to two main factors. The first is the ... network of vendors supplying FireflySci products all around the world. , 2016 was a ...
Breaking Biology Technology: