Navigation Links
Family trees for yeast cells
Date:5/13/2013

Researchers at the Institute for Systems Biology in Seattle (USA) and the Luxembourg Centre for Systems Biomedicine (LCSB) at the University of Luxembourg have jointly developed a revolutionary method to analyse the genomes of yeast families. The team of Dr. Aime Dudley from the ISB and Dr. Patrick May from LCSB published their paper in the renowned scientific journal Nature Methods on May 12th. It describes a new method called BEST: Barcode Enabled Sequencing of Tetrads (DOI: 10.1038/nmeth.2479).

Baker's yeast is one of the most powerful and widely used organisms in genetic research because of the ability to perform a technique called tetrad analysis. Matings between two yeast "parents" produce four yeast "children" or spores that stick together as "tetrads". To genetically analyse these progeny, one needs to physically separate ("dissect") the four spores of each tetrad. The current method for doing this is extremely labour-intensive and has remained essentially unchanged for 75 years. A number of methods have tried to circumvent this manual process, but until now, none has been able to capture a crucial piece of information, the tetrad relationships between the spores.

The research team designed a new method that is several hundred times faster and is based on the following principles: First, two DNA sequences are added to the cells: A short sequence, called a barcode, that labels spores that come from the same tetrad and additionally a GFP (Green Fluorescent Protein) reporter gene. This fluorescent reporter allows the separation of tetrads away from other types of yeast cells by FACS sorting. Then, the four spores of the tetrad are disrupted using a specific enzymatic reaction and allowed to grow up as individuals. Ultimately, the barcode and the same 2-3% of each individual's genome are sequenced allowing the spore strain to be placed in the correct position of the family tree for further genetic analysis.

"Identifying the genes that underlie complex traits requires extremely large numbers of progeny and high volumes of DNA sequencing," says Patrick May. "BEST combines the power of a gold standard genetic technique with ultra-high-throughput genome sequencing in a way that will enable the study of problems that were previously not possible."


'/>"/>

Contact: Sabine Mosch
sabine.mosch@uni.lu
352-466-644-6423
University of Luxembourg
Source:Eurekalert

Related biology news :

1. New research constructs ant family tree
2. Signalling pathways meeting targeting the HER/EGFR family: Focus on breast, lung and colorectal cancers
3. FASEB SRC announces conference: TGF-β Superfamily: Signaling in Development & Disease
4. FASEB SRC announces conference registration open for: Arf and Rab Family G Proteins
5. Bats split on family living
6. Networking ability a family trait in monkeys
7. All in the family: A genetic link between epilepsy and migraine
8. Bloomberg School receives $28 million for family planning advocacy
9. CEVA, Rubidium Partner to Deliver Speech Processing Solutions based on the CEVA-TeakLite Family of DSPs
10. Millipede family added to Australian fauna
11. Helping family is key for social birds
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/30/2017)... Today, American Trucking Associations announced Seeing ... and eye tracking software, became the newest member ... "Artificial intelligence and advanced sensing algorithms ... driver,s attentiveness levels while on the road.  Drivers ... fatigue and prevent potential accidents, which could lead ...
(Date:5/23/2017)... the first robotic gym for the rehabilitation and functional motor sense evaluation ... Genoa, Italy . The first 30 robots will be available from ... . The technology was developed and patented at the IIT laboratories ... Technology thanks to a 10 million euro investment from entrepreneur Sergio Dompè. ... ...
(Date:5/6/2017)... 5, 2017 RAM Group , ... new breakthrough in biometric authentication based on a ... properties to perform biometric authentication. These new sensors are ... created by Ram Group and its partners. This sensor ... supply chains and security. Ram Group is a ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... , Oct. 10, 2017 International research firm Parks Associates ... will speak at the TMA 2017 Annual Meeting , October 11 ... in the residential home security market and how smart safety and security ... Parks Associates: ... "The residential security ...
(Date:10/10/2017)... BARBARA, CALIFORNIA (PRWEB) , ... October 10, 2017 ... ... management, technological innovation and business process optimization firm for the life sciences and ... BoxWorks conference in San Francisco. , The presentation, “Automating GxP Validation ...
(Date:10/9/2017)... DIEGO , Oct. 9, 2017  BioTech ... biological mechanism by which its ProCell stem cell ... critical limb ischemia.  The Company, demonstrated that treatment ... amount of limbs saved as compared to standard ... the molecule HGF resulted in reduction of therapeutic ...
(Date:10/9/2017)... ... ... At its national board meeting in North Carolina, ARCS® Foundation President ... and Astronomy, has been selected for membership in ARCS Alumni Hall of Fame ... Prize in Fundamental physics for the discovery of the accelerating expansion of the universe, ...
Breaking Biology Technology: